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Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus.
Brain Res 2009; 1258:34-42BR

Abstract

Body fluid balance requires the release of arginine vasopressin (AVP) from the neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells (MNCs), which in turn are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic inputs and humoral factors such as plasma osmolality. Previous studies have shown that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic stress in the rat SON. In the present study, the effects of BDNF on excitatory and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF application caused a significant reduction in the frequency and amplitude of the spontaneous inhibitory postsynaptic currents of the MNCs without affecting the spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent protein (eGFP) transgene revealed that the amplitude of GABA-induced currents were significantly smaller after BDNF treatment. Moreover, multi-cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in the rat SON may decrease the postsynaptic GABAergic activity and may be involved in the regulatory mechanisms of body fluid homeostasis.

Authors+Show Affiliations

Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19150437

Citation

Ohbuchi, Toyoaki, et al. "Brain-derived Neurotrophic Factor Inhibits Spontaneous Inhibitory Postsynaptic Currents in the Rat Supraoptic Nucleus." Brain Research, vol. 1258, 2009, pp. 34-42.
Ohbuchi T, Yokoyama T, Saito T, et al. Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus. Brain Res. 2009;1258:34-42.
Ohbuchi, T., Yokoyama, T., Saito, T., Hashimoto, H., Suzuki, H., Otsubo, H., ... Ueta, Y. (2009). Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus. Brain Research, 1258, pp. 34-42. doi:10.1016/j.brainres.2008.12.057.
Ohbuchi T, et al. Brain-derived Neurotrophic Factor Inhibits Spontaneous Inhibitory Postsynaptic Currents in the Rat Supraoptic Nucleus. Brain Res. 2009 Mar 3;1258:34-42. PubMed PMID: 19150437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus. AU - Ohbuchi,Toyoaki, AU - Yokoyama,Toru, AU - Saito,Takeshi, AU - Hashimoto,Hirofumi, AU - Suzuki,Hitoshi, AU - Otsubo,Hiroki, AU - Fujihara,Hiroaki, AU - Suzuki,Hideaki, AU - Ueta,Yoichi, Y1 - 2008/12/31/ PY - 2008/09/14/received PY - 2008/12/11/revised PY - 2008/12/17/accepted PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/4/22/medline SP - 34 EP - 42 JF - Brain research JO - Brain Res. VL - 1258 N2 - Body fluid balance requires the release of arginine vasopressin (AVP) from the neurohypophysis. The hypothalamic supraoptic nucleus (SON) is one of the major sites for the synthesis of AVP, and secretion of AVP is controlled by the electrical activities of magnocellular neurosecretory cells (MNCs), which in turn are regulated by neuronal excitatory glutamatergic and inhibitory GABAergic inputs and humoral factors such as plasma osmolality. Previous studies have shown that brain-derived neurotrophic factor (BDNF) mRNA was increased by osmotic stress in the rat SON. In the present study, the effects of BDNF on excitatory and inhibitory synaptic inputs were examined in the MNCs of rat SON, using the whole-cell patch-clamp technique in in vitro brain slice preparations. BDNF application caused a significant reduction in the frequency and amplitude of the spontaneous inhibitory postsynaptic currents of the MNCs without affecting the spontaneous excitatory postsynaptic currents. Next, whole-cell patch-clamp recordings from dissociated SON MNCs expressing AVP-enhanced green fluorescent protein (eGFP) transgene revealed that the amplitude of GABA-induced currents were significantly smaller after BDNF treatment. Moreover, multi-cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed the expression of TrkB mRNA in AVP-eGFP neurons. These results suggest that BDNF in the rat SON may decrease the postsynaptic GABAergic activity and may be involved in the regulatory mechanisms of body fluid homeostasis. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/19150437/Brain_derived_neurotrophic_factor_inhibits_spontaneous_inhibitory_postsynaptic_currents_in_the_rat_supraoptic_nucleus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(08)03092-8 DB - PRIME DP - Unbound Medicine ER -