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Surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopy for sensitive detection of tumor markers.
Methods Mol Biol. 2009; 503:3-20.MM

Abstract

Surface plasmon resonance (SPR), which provides real-time, in situ analysis of dynamic surface events, is a valuable tool for studying interactions between biomolecules. In the clinical diagnosis of tumor markers in human blood, SPR is applied to detect the formation of a sandwich-type immune complex composed of a primary antibody immobilized on a sensor surface, the tumor marker, and a secondary antibody. However, the SPR signal is quite low due to the minute amounts (ng-pg/mL) of most tumor markers in blood. We have shown that the SPR signal can be amplified by applying an antibody against the secondary antibody or streptavidin-conjugated nanobeads that specifically accumulate on the secondary antibody. Another method employed for highly sensitive detection is the surface plasmon field-enhanced fluorescence spectroscopy-based immunoassay, which utilizes the enhanced electric field intensity at a metal/water interface to excite a fluorophore. Fluorescence intensity attributed to binding of a fluorophore-labeled secondary antibody is increased due to the enhanced field in the SPR condition and can be monitored in real time.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Arima Y
Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Teramura Y
No affiliation info available
Takiguchi H
No affiliation info available
Kawano K
No affiliation info available
Kotera H
No affiliation info available
Iwata H
No affiliation info available

MeSH

Biomarkers, TumorBiosensing TechniquesBlood Chemical AnalysisEquipment DesignEquipment Failure AnalysisHumansImmunoassayNeoplasm ProteinsNeoplasmsReproducibility of ResultsSensitivity and SpecificitySpectrometry, FluorescenceSurface Plasmon Resonance

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

19151933

Citation

Arima, Yusuke, et al. "Surface Plasmon Resonance and Surface Plasmon Field-enhanced Fluorescence Spectroscopy for Sensitive Detection of Tumor Markers." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 3-20.
Arima Y, Teramura Y, Takiguchi H, et al. Surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopy for sensitive detection of tumor markers. Methods Mol Biol. 2009;503:3-20.
Arima, Y., Teramura, Y., Takiguchi, H., Kawano, K., Kotera, H., & Iwata, H. (2009). Surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopy for sensitive detection of tumor markers. Methods in Molecular Biology (Clifton, N.J.), 503, 3-20. https://doi.org/10.1007/978-1-60327-567-5_1
Arima Y, et al. Surface Plasmon Resonance and Surface Plasmon Field-enhanced Fluorescence Spectroscopy for Sensitive Detection of Tumor Markers. Methods Mol Biol. 2009;503:3-20. PubMed PMID: 19151933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopy for sensitive detection of tumor markers. AU - Arima,Yusuke, AU - Teramura,Yuji, AU - Takiguchi,Hiromi, AU - Kawano,Keiko, AU - Kotera,Hidetoshi, AU - Iwata,Hiroo, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 3 EP - 20 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - Surface plasmon resonance (SPR), which provides real-time, in situ analysis of dynamic surface events, is a valuable tool for studying interactions between biomolecules. In the clinical diagnosis of tumor markers in human blood, SPR is applied to detect the formation of a sandwich-type immune complex composed of a primary antibody immobilized on a sensor surface, the tumor marker, and a secondary antibody. However, the SPR signal is quite low due to the minute amounts (ng-pg/mL) of most tumor markers in blood. We have shown that the SPR signal can be amplified by applying an antibody against the secondary antibody or streptavidin-conjugated nanobeads that specifically accumulate on the secondary antibody. Another method employed for highly sensitive detection is the surface plasmon field-enhanced fluorescence spectroscopy-based immunoassay, which utilizes the enhanced electric field intensity at a metal/water interface to excite a fluorophore. Fluorescence intensity attributed to binding of a fluorophore-labeled secondary antibody is increased due to the enhanced field in the SPR condition and can be monitored in real time. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151933/Surface_plasmon_resonance_and_surface_plasmon_field_enhanced_fluorescence_spectroscopy_for_sensitive_detection_of_tumor_markers_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_1 DB - PRIME DP - Unbound Medicine ER -