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Surface plasmon resonance biosensor for biomolecular interaction analysis based on spatial modulation phase detection.
Methods Mol Biol. 2009; 503:21-35.MM

Abstract

Surface plasmon resonance (SPR) biosensor is a powerful tool for biomolecular interaction analysis in proteomics research and drug discovery. But when it is used to analyze small molecules, the sensitivity still needs enhancement. Phase detection is a potential solution, for phase changes more abruptly than intensity when SPR is excited. An SPR system is developed based on spatial modulation phase detection (SMPD). In this system, collimated monochromatic light is used to excite SPR, and the phase of the reflected light is spatially modulated to generate an interference pattern. By processing the interference pattern by certain algorithms, instantaneous phase distribution of the whole sensing area can be obtained. Continuously detecting the phase change, the whole process of biomolecular interaction can be recorded in the form of phase change, based on which we can make kinetic analysis and get kinetic parameters. Antibody array chip is tested by this system. Experimental results indicate that this technique is capable of array detection and is more sensitive than intensity detection.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Ding X
Department of Precision Instruments and Mechanics, Tsinghua University, Beijing, China.
Liu F
No affiliation info available
Yu X
No affiliation info available

MeSH

AlgorithmsBiopolymersBiosensing TechniquesEquipment DesignEquipment Failure AnalysisImmunoassayProtein Array AnalysisProtein Interaction MappingRefractometryReproducibility of ResultsSensitivity and SpecificitySurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19151934

Citation

Ding, Xiang, et al. "Surface Plasmon Resonance Biosensor for Biomolecular Interaction Analysis Based On Spatial Modulation Phase Detection." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 21-35.
Ding X, Liu F, Yu X. Surface plasmon resonance biosensor for biomolecular interaction analysis based on spatial modulation phase detection. Methods Mol Biol. 2009;503:21-35.
Ding, X., Liu, F., & Yu, X. (2009). Surface plasmon resonance biosensor for biomolecular interaction analysis based on spatial modulation phase detection. Methods in Molecular Biology (Clifton, N.J.), 503, 21-35. https://doi.org/10.1007/978-1-60327-567-5_2
Ding X, Liu F, Yu X. Surface Plasmon Resonance Biosensor for Biomolecular Interaction Analysis Based On Spatial Modulation Phase Detection. Methods Mol Biol. 2009;503:21-35. PubMed PMID: 19151934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance biosensor for biomolecular interaction analysis based on spatial modulation phase detection. AU - Ding,Xiang, AU - Liu,Fangfang, AU - Yu,Xinglong, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 21 EP - 35 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - Surface plasmon resonance (SPR) biosensor is a powerful tool for biomolecular interaction analysis in proteomics research and drug discovery. But when it is used to analyze small molecules, the sensitivity still needs enhancement. Phase detection is a potential solution, for phase changes more abruptly than intensity when SPR is excited. An SPR system is developed based on spatial modulation phase detection (SMPD). In this system, collimated monochromatic light is used to excite SPR, and the phase of the reflected light is spatially modulated to generate an interference pattern. By processing the interference pattern by certain algorithms, instantaneous phase distribution of the whole sensing area can be obtained. Continuously detecting the phase change, the whole process of biomolecular interaction can be recorded in the form of phase change, based on which we can make kinetic analysis and get kinetic parameters. Antibody array chip is tested by this system. Experimental results indicate that this technique is capable of array detection and is more sensitive than intensity detection. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151934/Surface_plasmon_resonance_biosensor_for_biomolecular_interaction_analysis_based_on_spatial_modulation_phase_detection_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_2 DB - PRIME DP - Unbound Medicine ER -