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Array-based spectral SPR biosensor: analysis of mumps virus infection.
Methods Mol Biol. 2009; 503:37-47.MM

Abstract

Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed by Kretschmann geometry, based on the wavelength interrogation with various modules such as protein arrays, optical unit, programs for data acquisition and processing, and a motorized x-y stage for scanning. Protein arrays consist of glass/gold film/linker layer/protein/air. The surface of gold arrays was modified with poly(diallyldimethylammonium chloride) and 11-mercap-toundecanoic acid to immobilize mumps virus. The virus arrays were used to analyze anti-mumps virus in a buffer or human serum by the line-scanning mode of the spectral SPR biosensor. The array-based spectral SPR biosensor has a strong potential for a high-throughput serodiagnosis of infectious diseases.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yuk JS
Department of Molecular and Cellular Biochemistry and Nanobio Sensor Research Center, Kangwon National University College of Medicine, Chuncheon, Kangwon-do, Korea.
Ha KS
No affiliation info available

MeSH

AlgorithmsBiosensing TechniquesBlood Chemical AnalysisEquipment DesignEquipment Failure AnalysisHumansMicroarray AnalysisMumpsMumps virusReproducibility of ResultsSensitivity and SpecificitySurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19151935

Citation

Yuk, Jong Seol, and Kwon-Soo Ha. "Array-based Spectral SPR Biosensor: Analysis of Mumps Virus Infection." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 37-47.
Yuk JS, Ha KS. Array-based spectral SPR biosensor: analysis of mumps virus infection. Methods Mol Biol. 2009;503:37-47.
Yuk, J. S., & Ha, K. S. (2009). Array-based spectral SPR biosensor: analysis of mumps virus infection. Methods in Molecular Biology (Clifton, N.J.), 503, 37-47. https://doi.org/10.1007/978-1-60327-567-5_3
Yuk JS, Ha KS. Array-based Spectral SPR Biosensor: Analysis of Mumps Virus Infection. Methods Mol Biol. 2009;503:37-47. PubMed PMID: 19151935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Array-based spectral SPR biosensor: analysis of mumps virus infection. AU - Yuk,Jong Seol, AU - Ha,Kwon-Soo, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 37 EP - 47 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed by Kretschmann geometry, based on the wavelength interrogation with various modules such as protein arrays, optical unit, programs for data acquisition and processing, and a motorized x-y stage for scanning. Protein arrays consist of glass/gold film/linker layer/protein/air. The surface of gold arrays was modified with poly(diallyldimethylammonium chloride) and 11-mercap-toundecanoic acid to immobilize mumps virus. The virus arrays were used to analyze anti-mumps virus in a buffer or human serum by the line-scanning mode of the spectral SPR biosensor. The array-based spectral SPR biosensor has a strong potential for a high-throughput serodiagnosis of infectious diseases. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151935/Array_based_spectral_SPR_biosensor:_analysis_of_mumps_virus_infection_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_3 DB - PRIME DP - Unbound Medicine ER -