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Simple luminescence detector for capillary electrophoresis.
Methods Mol Biol. 2009; 503:221-37.MM

Abstract

The performance of a homemade, simple, fluorescence-induced capillary electrophoresis (CE) detector is described here. It is based on LED as excitation source, a bifurcated optical fibre as a waveguide and a CCD as a photodetector. The connection of all the components is fairly easy, even for non-experts. This detector provides a low cost and rapid system for the determination of high-quantum-yield native fluorescence compounds and fluorescence derivatised compounds by CE with direct fluorescence determination. R-phycoerythrin and B-phycoerythrin were used as models for native fluorescence compounds and amine labelled with FITC were set as models for the fluorescence derivatised ones. Detection limits of 0.50 and 0.64 microg/mL for R-phycoerythrin and B-phycoerythrin and 1.6 x 10-(7) M for FITC-labelled 1,6-diaminohexane were achieved. The homemade LED-IF detector is not expected to displace the LIF-IF one, but offers another possibility and a cheaper way to solve simple analytical problems for determining biomolecules.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19151944

Citation

Segura-Carretero, Antonio, et al. "Simple Luminescence Detector for Capillary Electrophoresis." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 221-37.
Segura-Carretero A, Fernández-Sánchez JF, Fernández-Gutiérrez A. Simple luminescence detector for capillary electrophoresis. Methods Mol Biol. 2009;503:221-37.
Segura-Carretero, A., Fernández-Sánchez, J. F., & Fernández-Gutiérrez, A. (2009). Simple luminescence detector for capillary electrophoresis. Methods in Molecular Biology (Clifton, N.J.), 503, 221-37. https://doi.org/10.1007/978-1-60327-567-5_12
Segura-Carretero A, Fernández-Sánchez JF, Fernández-Gutiérrez A. Simple Luminescence Detector for Capillary Electrophoresis. Methods Mol Biol. 2009;503:221-37. PubMed PMID: 19151944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple luminescence detector for capillary electrophoresis. AU - Segura-Carretero,Antonio, AU - Fernández-Sánchez,Jorge F, AU - Fernández-Gutiérrez,Alberto, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 221 EP - 37 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - The performance of a homemade, simple, fluorescence-induced capillary electrophoresis (CE) detector is described here. It is based on LED as excitation source, a bifurcated optical fibre as a waveguide and a CCD as a photodetector. The connection of all the components is fairly easy, even for non-experts. This detector provides a low cost and rapid system for the determination of high-quantum-yield native fluorescence compounds and fluorescence derivatised compounds by CE with direct fluorescence determination. R-phycoerythrin and B-phycoerythrin were used as models for native fluorescence compounds and amine labelled with FITC were set as models for the fluorescence derivatised ones. Detection limits of 0.50 and 0.64 microg/mL for R-phycoerythrin and B-phycoerythrin and 1.6 x 10-(7) M for FITC-labelled 1,6-diaminohexane were achieved. The homemade LED-IF detector is not expected to displace the LIF-IF one, but offers another possibility and a cheaper way to solve simple analytical problems for determining biomolecules. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151944/Simple_luminescence_detector_for_capillary_electrophoresis_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_12 DB - PRIME DP - Unbound Medicine ER -