Segura-Carretero, Antonio, et al. "Simple Luminescence Detector for Capillary Electrophoresis." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 221-37.
Segura-Carretero A, Fernández-Sánchez JF, Fernández-Gutiérrez A. Simple luminescence detector for capillary electrophoresis. Methods Mol Biol. 2009;503:221-37.
Segura-Carretero, A., Fernández-Sánchez, J. F., & Fernández-Gutiérrez, A. (2009). Simple luminescence detector for capillary electrophoresis. Methods in Molecular Biology (Clifton, N.J.), 503, 221-37. https://doi.org/10.1007/978-1-60327-567-5_12
Segura-Carretero A, Fernández-Sánchez JF, Fernández-Gutiérrez A. Simple Luminescence Detector for Capillary Electrophoresis. Methods Mol Biol. 2009;503:221-37. PubMed PMID: 19151944.
TY - JOUR
T1 - Simple luminescence detector for capillary electrophoresis.
AU - Segura-Carretero,Antonio,
AU - Fernández-Sánchez,Jorge F,
AU - Fernández-Gutiérrez,Alberto,
PY - 2009/1/20/entrez
PY - 2009/1/20/pubmed
PY - 2009/3/20/medline
SP - 221
EP - 37
JF - Methods in molecular biology (Clifton, N.J.)
JO - Methods Mol Biol
VL - 503
N2 - The performance of a homemade, simple, fluorescence-induced capillary electrophoresis (CE) detector is described here. It is based on LED as excitation source, a bifurcated optical fibre as a waveguide and a CCD as a photodetector. The connection of all the components is fairly easy, even for non-experts. This detector provides a low cost and rapid system for the determination of high-quantum-yield native fluorescence compounds and fluorescence derivatised compounds by CE with direct fluorescence determination. R-phycoerythrin and B-phycoerythrin were used as models for native fluorescence compounds and amine labelled with FITC were set as models for the fluorescence derivatised ones. Detection limits of 0.50 and 0.64 microg/mL for R-phycoerythrin and B-phycoerythrin and 1.6 x 10-(7) M for FITC-labelled 1,6-diaminohexane were achieved. The homemade LED-IF detector is not expected to displace the LIF-IF one, but offers another possibility and a cheaper way to solve simple analytical problems for determining biomolecules.
SN - 1064-3745
UR - https://www.unboundmedicine.com/medline/citation/19151944/Simple_luminescence_detector_for_capillary_electrophoresis_
L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_12
DB - PRIME
DP - Unbound Medicine