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Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria.
Methods Mol Biol. 2009; 503:325-35.MM

Abstract

An integrated circuit (IC) of photodiode array (PDA) microchip system was used for the on-chip detection of E. coli O157:H7 based on an enzymatic bioassay and light absorption property of the reaction product. The PDA microchip consisting of an array of 12 x 12 photodiode detection elements served as a photosensor as well as a protein-immobilizing sample platform. As a result, E. coli O157:H7 could be detected directly on the surface of PDA detection elements. E. coli O157:H7 was detected by forming a "sandwich-type" enzymatic immunocomplex on the PDA detection elements using an on-chip bioassay. The quantitative analysis of E. coli O157:H7 immunocomplex was carried out based on the light absorption property of the enzymatic reaction products of E. coli O157:H7 immunocomplexes with respect to a red beam produced by light emitting diodes (LEDs) installed right above the PDA microchip. During the on-chip bioassay, the wet photodiode detection elements exposed to a lot of biological materials or buffer solutions were capable of maintaining their photosensing capabilities. The portable PDA on-chip biosensor permits direct optical detection of E. coli O157:H7 and eliminates the necessity of the conventional expensive microplate reader that is incompatible with the size of the protein microarray.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Song JM
Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul, South Korea.
Kwon HT
No affiliation info available

MeSH

Biosensing TechniquesColony Count, MicrobialElectronicsEquipment DesignEquipment Failure AnalysisEscherichia coli O157Microarray AnalysisPhotometryReproducibility of ResultsSemiconductorsSensitivity and Specificity

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19151950

Citation

Song, Joon Myong, and Ho Taik Kwon. "Photodiode Array On-chip Biosensor for the Detection of E. Coli O157:H7 Pathogenic Bacteria." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 325-35.
Song JM, Kwon HT. Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria. Methods Mol Biol. 2009;503:325-35.
Song, J. M., & Kwon, H. T. (2009). Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria. Methods in Molecular Biology (Clifton, N.J.), 503, 325-35. https://doi.org/10.1007/978-1-60327-567-5_18
Song JM, Kwon HT. Photodiode Array On-chip Biosensor for the Detection of E. Coli O157:H7 Pathogenic Bacteria. Methods Mol Biol. 2009;503:325-35. PubMed PMID: 19151950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria. AU - Song,Joon Myong, AU - Kwon,Ho Taik, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 325 EP - 35 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - An integrated circuit (IC) of photodiode array (PDA) microchip system was used for the on-chip detection of E. coli O157:H7 based on an enzymatic bioassay and light absorption property of the reaction product. The PDA microchip consisting of an array of 12 x 12 photodiode detection elements served as a photosensor as well as a protein-immobilizing sample platform. As a result, E. coli O157:H7 could be detected directly on the surface of PDA detection elements. E. coli O157:H7 was detected by forming a "sandwich-type" enzymatic immunocomplex on the PDA detection elements using an on-chip bioassay. The quantitative analysis of E. coli O157:H7 immunocomplex was carried out based on the light absorption property of the enzymatic reaction products of E. coli O157:H7 immunocomplexes with respect to a red beam produced by light emitting diodes (LEDs) installed right above the PDA microchip. During the on-chip bioassay, the wet photodiode detection elements exposed to a lot of biological materials or buffer solutions were capable of maintaining their photosensing capabilities. The portable PDA on-chip biosensor permits direct optical detection of E. coli O157:H7 and eliminates the necessity of the conventional expensive microplate reader that is incompatible with the size of the protein microarray. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151950/Photodiode_array_on_chip_biosensor_for_the_detection_of_E__coli_O157:H7_pathogenic_bacteria_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_18 DB - PRIME DP - Unbound Medicine ER -