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Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria.
Methods Mol Biol. 2009; 503:325-35.MM

Abstract

An integrated circuit (IC) of photodiode array (PDA) microchip system was used for the on-chip detection of E. coli O157:H7 based on an enzymatic bioassay and light absorption property of the reaction product. The PDA microchip consisting of an array of 12 x 12 photodiode detection elements served as a photosensor as well as a protein-immobilizing sample platform. As a result, E. coli O157:H7 could be detected directly on the surface of PDA detection elements. E. coli O157:H7 was detected by forming a "sandwich-type" enzymatic immunocomplex on the PDA detection elements using an on-chip bioassay. The quantitative analysis of E. coli O157:H7 immunocomplex was carried out based on the light absorption property of the enzymatic reaction products of E. coli O157:H7 immunocomplexes with respect to a red beam produced by light emitting diodes (LEDs) installed right above the PDA microchip. During the on-chip bioassay, the wet photodiode detection elements exposed to a lot of biological materials or buffer solutions were capable of maintaining their photosensing capabilities. The portable PDA on-chip biosensor permits direct optical detection of E. coli O157:H7 and eliminates the necessity of the conventional expensive microplate reader that is incompatible with the size of the protein microarray.

Authors+Show Affiliations

Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul, South Korea.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19151950

Citation

Song, Joon Myong, and Ho Taik Kwon. "Photodiode Array On-chip Biosensor for the Detection of E. Coli O157:H7 Pathogenic Bacteria." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 325-35.
Song JM, Kwon HT. Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria. Methods Mol Biol. 2009;503:325-35.
Song, J. M., & Kwon, H. T. (2009). Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria. Methods in Molecular Biology (Clifton, N.J.), 503, 325-35. https://doi.org/10.1007/978-1-60327-567-5_18
Song JM, Kwon HT. Photodiode Array On-chip Biosensor for the Detection of E. Coli O157:H7 Pathogenic Bacteria. Methods Mol Biol. 2009;503:325-35. PubMed PMID: 19151950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photodiode array on-chip biosensor for the detection of E. coli O157:H7 pathogenic bacteria. AU - Song,Joon Myong, AU - Kwon,Ho Taik, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 325 EP - 35 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - An integrated circuit (IC) of photodiode array (PDA) microchip system was used for the on-chip detection of E. coli O157:H7 based on an enzymatic bioassay and light absorption property of the reaction product. The PDA microchip consisting of an array of 12 x 12 photodiode detection elements served as a photosensor as well as a protein-immobilizing sample platform. As a result, E. coli O157:H7 could be detected directly on the surface of PDA detection elements. E. coli O157:H7 was detected by forming a "sandwich-type" enzymatic immunocomplex on the PDA detection elements using an on-chip bioassay. The quantitative analysis of E. coli O157:H7 immunocomplex was carried out based on the light absorption property of the enzymatic reaction products of E. coli O157:H7 immunocomplexes with respect to a red beam produced by light emitting diodes (LEDs) installed right above the PDA microchip. During the on-chip bioassay, the wet photodiode detection elements exposed to a lot of biological materials or buffer solutions were capable of maintaining their photosensing capabilities. The portable PDA on-chip biosensor permits direct optical detection of E. coli O157:H7 and eliminates the necessity of the conventional expensive microplate reader that is incompatible with the size of the protein microarray. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151950/Photodiode_array_on_chip_biosensor_for_the_detection_of_E__coli_O157:H7_pathogenic_bacteria_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_18 DB - PRIME DP - Unbound Medicine ER -