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Integrating waveguide biosensor.
Methods Mol Biol. 2009; 503:389-401.MM

Abstract

The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed using Salmonella as model. The analyte was immunocaptured on the inner surface of the waveguide and then detected by the antibody conjugated with fluorescent dye. The waveguide was illuminated by an excitation light at a 90 degrees angle. The emitted light from fluorescent labels on the surface of the waveguide was efficiently collected and channeled to a detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing fluorescent dye Cy5, a 635-nm diode laser for excitation, and a photomultiplier tube detector, the Integrating Waveguide Sensor System was able to detect approximately ten captured cells of Salmonella.

Authors+Show Affiliations

Creatv MicroTech Inc, Potomac, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19151954

Citation

Li, Shuhong, et al. "Integrating Waveguide Biosensor." Methods in Molecular Biology (Clifton, N.J.), vol. 503, 2009, pp. 389-401.
Li S, Amstutz P, Tang CM, et al. Integrating waveguide biosensor. Methods Mol Biol. 2009;503:389-401.
Li, S., Amstutz, P., Tang, C. M., Hang, J., Zhu, P., Zhang, Y., Shelton, D. R., & Karns, J. S. (2009). Integrating waveguide biosensor. Methods in Molecular Biology (Clifton, N.J.), 503, 389-401. https://doi.org/10.1007/978-1-60327-567-5_22
Li S, et al. Integrating Waveguide Biosensor. Methods Mol Biol. 2009;503:389-401. PubMed PMID: 19151954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrating waveguide biosensor. AU - Li,Shuhong, AU - Amstutz,Platte,3rd AU - Tang,Cha-Mei, AU - Hang,Jun, AU - Zhu,Peixuan, AU - Zhang,Yunqi, AU - Shelton,Daniel R, AU - Karns,Jeffrey S, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/20/medline SP - 389 EP - 401 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 503 N2 - The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed using Salmonella as model. The analyte was immunocaptured on the inner surface of the waveguide and then detected by the antibody conjugated with fluorescent dye. The waveguide was illuminated by an excitation light at a 90 degrees angle. The emitted light from fluorescent labels on the surface of the waveguide was efficiently collected and channeled to a detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing fluorescent dye Cy5, a 635-nm diode laser for excitation, and a photomultiplier tube detector, the Integrating Waveguide Sensor System was able to detect approximately ten captured cells of Salmonella. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19151954/Integrating_waveguide_biosensor_ L2 - https://dx.doi.org/10.1007/978-1-60327-567-5_22 DB - PRIME DP - Unbound Medicine ER -