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The simplified technique of high resolution two-dimensional polyacrylamide gel electrophoresis: biomedical applications in health and disease.
Electrophoresis. 1991 Jul-Aug; 12(7-8):461-71.E

Abstract

The application of our simplified technique of high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to human body fluids is reviewed. Serum/plasma protein changes associated with alcohol abuse, familial dyslipoproteinemia ("fish-eye" disease), and myocardial infarction are demonstrated. High resolution 2-D PAGE of amniotic fluid, cerebrospinal fluid, urine, and saliva is shown with reference to the work of others, and the detection of pink-violet staining "lumicarmines" in sweat and tear fluid is reported for the first time. General aspects relating to the methodology are discussed. These include sample preparation, the choice of electrophoresis conditions (denaturing or nondenaturing) and detection method (Coomassie Brilliant Blue or silver), and the effects of native protein pretreatment with sodium dodecyl sulfate prior to silver staining or isoelectric focusing gel shrinkage in glycerol prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors+Show Affiliations

Marshall T
Biochemistry Research Laboratory, School of Pharmaceutical & Chemical Sciences, Sunderland Polytechnic, Green Terrace, Great Britain.
Williams KM
No affiliation info available

MeSH

AlcoholismBlood ProteinsElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelHumansIsoelectric FocusingMolecular WeightMyocardial InfarctionProteinsProteinuriaReference ValuesSalivary Proteins and PeptidesSweatTears

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1915239

Citation

Marshall, T, and K M. Williams. "The Simplified Technique of High Resolution Two-dimensional Polyacrylamide Gel Electrophoresis: Biomedical Applications in Health and Disease." Electrophoresis, vol. 12, no. 7-8, 1991, pp. 461-71.
Marshall T, Williams KM. The simplified technique of high resolution two-dimensional polyacrylamide gel electrophoresis: biomedical applications in health and disease. Electrophoresis. 1991;12(7-8):461-71.
Marshall, T., & Williams, K. M. (1991). The simplified technique of high resolution two-dimensional polyacrylamide gel electrophoresis: biomedical applications in health and disease. Electrophoresis, 12(7-8), 461-71.
Marshall T, Williams KM. The Simplified Technique of High Resolution Two-dimensional Polyacrylamide Gel Electrophoresis: Biomedical Applications in Health and Disease. Electrophoresis. 1991 Jul-Aug;12(7-8):461-71. PubMed PMID: 1915239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The simplified technique of high resolution two-dimensional polyacrylamide gel electrophoresis: biomedical applications in health and disease. AU - Marshall,T, AU - Williams,K M, PY - 1991/7/1/pubmed PY - 1991/7/1/medline PY - 1991/7/1/entrez SP - 461 EP - 71 JF - Electrophoresis JO - Electrophoresis VL - 12 IS - 7-8 N2 - The application of our simplified technique of high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to human body fluids is reviewed. Serum/plasma protein changes associated with alcohol abuse, familial dyslipoproteinemia ("fish-eye" disease), and myocardial infarction are demonstrated. High resolution 2-D PAGE of amniotic fluid, cerebrospinal fluid, urine, and saliva is shown with reference to the work of others, and the detection of pink-violet staining "lumicarmines" in sweat and tear fluid is reported for the first time. General aspects relating to the methodology are discussed. These include sample preparation, the choice of electrophoresis conditions (denaturing or nondenaturing) and detection method (Coomassie Brilliant Blue or silver), and the effects of native protein pretreatment with sodium dodecyl sulfate prior to silver staining or isoelectric focusing gel shrinkage in glycerol prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/1915239/The_simplified_technique_of_high_resolution_two_dimensional_polyacrylamide_gel_electrophoresis:_biomedical_applications_in_health_and_disease_ DB - PRIME DP - Unbound Medicine ER -