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Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes.
Genetics. 2009 Apr; 181(4):1627-38.G

Abstract

Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to M. oryzae, we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M. oryzae. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to M. oryzae. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M. oryzae.

Authors+Show Affiliations

Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19153255

Citation

Lee, Sang-Kyu, et al. "Rice Pi5-mediated Resistance to Magnaporthe Oryzae Requires the Presence of Two Coiled-coil-nucleotide-binding-leucine-rich Repeat Genes." Genetics, vol. 181, no. 4, 2009, pp. 1627-38.
Lee SK, Song MY, Seo YS, et al. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics. 2009;181(4):1627-38.
Lee, S. K., Song, M. Y., Seo, Y. S., Kim, H. K., Ko, S., Cao, P. J., Suh, J. P., Yi, G., Roh, J. H., Lee, S., An, G., Hahn, T. R., Wang, G. L., Ronald, P., & Jeon, J. S. (2009). Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics, 181(4), 1627-38. https://doi.org/10.1534/genetics.108.099226
Lee SK, et al. Rice Pi5-mediated Resistance to Magnaporthe Oryzae Requires the Presence of Two Coiled-coil-nucleotide-binding-leucine-rich Repeat Genes. Genetics. 2009;181(4):1627-38. PubMed PMID: 19153255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. AU - Lee,Sang-Kyu, AU - Song,Min-Young, AU - Seo,Young-Su, AU - Kim,Hye-Kyung, AU - Ko,Seho, AU - Cao,Pei-Jian, AU - Suh,Jung-Pil, AU - Yi,Gihwan, AU - Roh,Jae-Hwan, AU - Lee,Sichul, AU - An,Gynheung, AU - Hahn,Tae-Ryong, AU - Wang,Guo-Liang, AU - Ronald,Pamela, AU - Jeon,Jong-Seong, Y1 - 2009/01/19/ PY - 2009/1/21/entrez PY - 2009/1/21/pubmed PY - 2009/7/16/medline SP - 1627 EP - 38 JF - Genetics JO - Genetics VL - 181 IS - 4 N2 - Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to M. oryzae, we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M. oryzae. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to M. oryzae. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M. oryzae. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/19153255/Rice_Pi5_mediated_resistance_to_Magnaporthe_oryzae_requires_the_presence_of_two_coiled_coil_nucleotide_binding_leucine_rich_repeat_genes_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=19153255 DB - PRIME DP - Unbound Medicine ER -