Tags

Type your tag names separated by a space and hit enter

Broad spectrum antiviral fractions from the lichen Ramalina farinacea (L.) Ach.

Abstract

BACKGROUND

An ethylacetate-soluble fraction (ET4) from the lichen Ramalina farinacea has previously been shown to inhibit the infectivity of lentiviral and adenoviral vectors, as well as wild-type HIV-1. We now determined the antiviral activity of ET4 against other wild-type viruses, including the herpes simplex virus type 1 (HSV-1) and the respiratory syncytial virus (RSV).

METHODS

Wild-type HIV-1, HSV-1 or RSV were pre-incubated with various concentrations of ET4 for 30 min at 37 degrees C before adding to P4CCR5 indicator cell line (HIV-1), ELVIS TM indicator cell line (HSV-1) or HEp2 cell line (RSV) in 96-well microtitre plates. Controls contain virus alone without ET4. The anti-HIV and anti-HSV activities were quantified by estimating beta-galactosidase expression of the respective indicator cell lines while the anti-RSV activity was determined via an immunofluorescent technique, employing monoclonal mouse antibody against the P-protein of RSV. Toxicity of ET4 to cell lines was evaluated in parallel using either the BrdU incorporation method or the MTT method. The effect of ET4 on the enzymatic activity of HIV-1 reverse transcriptase was also evaluated using a chemiluminescent reverse transcriptase assay. Bioassay-guided fractionation of the whole methanol extract of R. farinacea involved sequential screening of HPLC fractions using a vector-based assay technique.

RESULTS

ET4 inhibited HSV-1 and RSV potently (IC(50)=6.09 and 3.65 microg/ml, respectively). Time-of-addition studies suggest that both entry and post-entry steps of the HIV-1 replication cycle and the entry step of the RSV replication cycle are targeted. Furthermore, ET4 inhibited HIV-1 reverse transcriptase with an IC(50) of 0.022 microg/ml. Bioassay-guided fractionation of ET4 led to the identification sub-fraction rfO, with activity against lentiviral vector and HIV-1 (RNA viruses) but not against HSV-1 (DNA virus) and sub-fraction rfM, with activity against HSV-1 but not against the lentiviral vector.

CONCLUSIONS

ET4 represents a novel fraction from the lichen R. farinacea with broad spectrum antiviral activity against DNA viruses (adenovirus and HSV-1) and RNA viruses (HIV-1 and RSV). The effect against DNA and RNA viruses is mediated by different sub-fractions within R. farinacea.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Molecular and Medical Virology, Ruhr University, Bochum, Germany. coesimone@yahoo.com

    , , , ,

    Source

    Chemotherapy 55:2 2009 pg 119-26

    MeSH

    Antiviral Agents
    HIV-1
    Herpesvirus 1, Human
    Lichens
    Respiratory Syncytial Viruses
    Virus Replication

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19155620

    Citation

    Esimone, C O., et al. "Broad Spectrum Antiviral Fractions From the Lichen Ramalina Farinacea (L.) Ach." Chemotherapy, vol. 55, no. 2, 2009, pp. 119-26.
    Esimone CO, Grunwald T, Nworu CS, et al. Broad spectrum antiviral fractions from the lichen Ramalina farinacea (L.) Ach. Chemotherapy. 2009;55(2):119-26.
    Esimone, C. O., Grunwald, T., Nworu, C. S., Kuate, S., Proksch, P., & Uberla, K. (2009). Broad spectrum antiviral fractions from the lichen Ramalina farinacea (L.) Ach. Chemotherapy, 55(2), pp. 119-26. doi:10.1159/000194974.
    Esimone CO, et al. Broad Spectrum Antiviral Fractions From the Lichen Ramalina Farinacea (L.) Ach. Chemotherapy. 2009;55(2):119-26. PubMed PMID: 19155620.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Broad spectrum antiviral fractions from the lichen Ramalina farinacea (L.) Ach. AU - Esimone,C O, AU - Grunwald,T, AU - Nworu,C S, AU - Kuate,S, AU - Proksch,P, AU - Uberla,K, Y1 - 2009/01/21/ PY - 2008/05/09/received PY - 2008/11/25/accepted PY - 2009/1/22/entrez PY - 2009/1/22/pubmed PY - 2009/3/26/medline SP - 119 EP - 26 JF - Chemotherapy JO - Chemotherapy VL - 55 IS - 2 N2 - BACKGROUND: An ethylacetate-soluble fraction (ET4) from the lichen Ramalina farinacea has previously been shown to inhibit the infectivity of lentiviral and adenoviral vectors, as well as wild-type HIV-1. We now determined the antiviral activity of ET4 against other wild-type viruses, including the herpes simplex virus type 1 (HSV-1) and the respiratory syncytial virus (RSV). METHODS: Wild-type HIV-1, HSV-1 or RSV were pre-incubated with various concentrations of ET4 for 30 min at 37 degrees C before adding to P4CCR5 indicator cell line (HIV-1), ELVIS TM indicator cell line (HSV-1) or HEp2 cell line (RSV) in 96-well microtitre plates. Controls contain virus alone without ET4. The anti-HIV and anti-HSV activities were quantified by estimating beta-galactosidase expression of the respective indicator cell lines while the anti-RSV activity was determined via an immunofluorescent technique, employing monoclonal mouse antibody against the P-protein of RSV. Toxicity of ET4 to cell lines was evaluated in parallel using either the BrdU incorporation method or the MTT method. The effect of ET4 on the enzymatic activity of HIV-1 reverse transcriptase was also evaluated using a chemiluminescent reverse transcriptase assay. Bioassay-guided fractionation of the whole methanol extract of R. farinacea involved sequential screening of HPLC fractions using a vector-based assay technique. RESULTS: ET4 inhibited HSV-1 and RSV potently (IC(50)=6.09 and 3.65 microg/ml, respectively). Time-of-addition studies suggest that both entry and post-entry steps of the HIV-1 replication cycle and the entry step of the RSV replication cycle are targeted. Furthermore, ET4 inhibited HIV-1 reverse transcriptase with an IC(50) of 0.022 microg/ml. Bioassay-guided fractionation of ET4 led to the identification sub-fraction rfO, with activity against lentiviral vector and HIV-1 (RNA viruses) but not against HSV-1 (DNA virus) and sub-fraction rfM, with activity against HSV-1 but not against the lentiviral vector. CONCLUSIONS: ET4 represents a novel fraction from the lichen R. farinacea with broad spectrum antiviral activity against DNA viruses (adenovirus and HSV-1) and RNA viruses (HIV-1 and RSV). The effect against DNA and RNA viruses is mediated by different sub-fractions within R. farinacea. SN - 1421-9794 UR - https://www.unboundmedicine.com/medline/citation/19155620/Broad_spectrum_antiviral_fractions_from_the_lichen_Ramalina_farinacea__L___Ach_ L2 - https://www.karger.com?DOI=10.1159/000194974 DB - PRIME DP - Unbound Medicine ER -