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Treponema pallidum distribution patterns in mucocutaneous lesions of primary and secondary syphilis: an immunohistochemical and ultrastructural study.
Hum Pathol. 2009 May; 40(5):624-30.HP

Abstract

To study the different patterns of Treponema pallidum distribution in primary and secondary syphilis, 34 biopsy specimens of 8 patients with primary and 26 with secondary syphilis were assessed. Histopathological features, silver stain, and immunohistochemical T pallidum polyclonal antibody expression were investigated. The number and distribution of spirochetes were evaluated, and ultrastructural studies were performed. Spirochetes were identified with Warthin-Starry stain in 17 specimens (4/8 primary and 13/26 secondary syphilis), whereas immunohistochemical analysis disclosed spirochetes in 29 (8/8 primary and 21/26 secondary syphilis). In secondary syphilis, an epitheliotropic pattern characterized by abundant spirochetes in the lower mucosa/epidermis in an intercellular distribution was observed. In contrast, primary syphilis exhibited a mixed epitheliotropic and vasculotropic pattern further manifested by treponemes surrounding the vascular walls. These differences were statistically significant. Ultrastructural examination confirmed these results. Immunohistochemistry shows greater sensitivity when compared with Warthin-Starry staining. The immunohistochemical pattern of T pallidum distribution may permit the diagnostic differentiation of primary from secondary syphilis.

Authors+Show Affiliations

Department of Dermatology, Hospital del Mar-IMAS, Barcelona, 08003, Spain. gmartin@imas.imim.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19157499

Citation

Martín-Ezquerra, Gemma, et al. "Treponema Pallidum Distribution Patterns in Mucocutaneous Lesions of Primary and Secondary Syphilis: an Immunohistochemical and Ultrastructural Study." Human Pathology, vol. 40, no. 5, 2009, pp. 624-30.
Martín-Ezquerra G, Fernandez-Casado A, Barco D, et al. Treponema pallidum distribution patterns in mucocutaneous lesions of primary and secondary syphilis: an immunohistochemical and ultrastructural study. Hum Pathol. 2009;40(5):624-30.
Martín-Ezquerra, G., Fernandez-Casado, A., Barco, D., Jucglà, A., Juanpere-Rodero, N., Manresa, J. M., de Almeida, L. M., Rodríguez-Peralto, J. L., Kutzner, H., Cerroni, L., Barranco, C., Lloreta, J., Requena, L., & Pujol, R. M. (2009). Treponema pallidum distribution patterns in mucocutaneous lesions of primary and secondary syphilis: an immunohistochemical and ultrastructural study. Human Pathology, 40(5), 624-30. https://doi.org/10.1016/j.humpath.2008.10.017
Martín-Ezquerra G, et al. Treponema Pallidum Distribution Patterns in Mucocutaneous Lesions of Primary and Secondary Syphilis: an Immunohistochemical and Ultrastructural Study. Hum Pathol. 2009;40(5):624-30. PubMed PMID: 19157499.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Treponema pallidum distribution patterns in mucocutaneous lesions of primary and secondary syphilis: an immunohistochemical and ultrastructural study. AU - Martín-Ezquerra,Gemma, AU - Fernandez-Casado,Alex, AU - Barco,Dídac, AU - Jucglà,Anna, AU - Juanpere-Rodero,Núria, AU - Manresa,Josep Maria, AU - de Almeida,Luis Miguel Soares, AU - Rodríguez-Peralto,Jose Luis, AU - Kutzner,Heinz, AU - Cerroni,Lorenzo, AU - Barranco,Carles, AU - Lloreta,Josep, AU - Requena,Luis, AU - Pujol,Ramon M, Y1 - 2009/01/20/ PY - 2008/07/08/received PY - 2008/10/02/revised PY - 2008/10/07/accepted PY - 2009/1/23/entrez PY - 2009/1/23/pubmed PY - 2009/6/6/medline SP - 624 EP - 30 JF - Human pathology JO - Hum Pathol VL - 40 IS - 5 N2 - To study the different patterns of Treponema pallidum distribution in primary and secondary syphilis, 34 biopsy specimens of 8 patients with primary and 26 with secondary syphilis were assessed. Histopathological features, silver stain, and immunohistochemical T pallidum polyclonal antibody expression were investigated. The number and distribution of spirochetes were evaluated, and ultrastructural studies were performed. Spirochetes were identified with Warthin-Starry stain in 17 specimens (4/8 primary and 13/26 secondary syphilis), whereas immunohistochemical analysis disclosed spirochetes in 29 (8/8 primary and 21/26 secondary syphilis). In secondary syphilis, an epitheliotropic pattern characterized by abundant spirochetes in the lower mucosa/epidermis in an intercellular distribution was observed. In contrast, primary syphilis exhibited a mixed epitheliotropic and vasculotropic pattern further manifested by treponemes surrounding the vascular walls. These differences were statistically significant. Ultrastructural examination confirmed these results. Immunohistochemistry shows greater sensitivity when compared with Warthin-Starry staining. The immunohistochemical pattern of T pallidum distribution may permit the diagnostic differentiation of primary from secondary syphilis. SN - 1532-8392 UR - https://www.unboundmedicine.com/medline/citation/19157499/Treponema_pallidum_distribution_patterns_in_mucocutaneous_lesions_of_primary_and_secondary_syphilis:_an_immunohistochemical_and_ultrastructural_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0046-8177(08)00510-8 DB - PRIME DP - Unbound Medicine ER -