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Gender affects liver desaturase expression in a rat model of n-3 fatty acid repletion.
J Nutr Biochem. 2010 Mar; 21(3):180-7.JN

Abstract

Dietary n-3 polyunsaturated fatty acids (PUFA) are major components of cell membranes and have beneficial effects on human health. Docosahexaenoic acid (DHA; 22:6n-3) is the most biologically important n-3 PUFA and can be synthesized from its dietary essential precursor, alpha-linolenic acid (ALA; 18:3n-3). Gender differences in the efficiency of DHA bioconversion have been reported, but underlying molecular mechanisms are unknown. We compared the capacity for DHA synthesis from ALA and the expression of related enzymes in the liver and cerebral cortex between male and female rats. Wistar rats, born with a low-DHA status, were supplied with a suboptimal amount of ALA from weaning to 8 weeks of age. Fatty acid composition was determined by gas chromatography, the mRNA expression of different genes involved in PUFA metabolism was determined by RT-PCR (low-density array) and the expression of proteins was determined by Western blot analysis. At 8 weeks, DHA content was higher (+20 to +40%) in each phospholipid class of female livers compared to male livers. The "Delta4," Delta5 and Delta6 desaturation indexes were 1.2-3 times higher in females than in males. The mRNA expression of Delta5- and Delta6-desaturase genes was 3.8 and 2.5 times greater, respectively, and the Delta5-desaturase protein was higher in female livers (+50%). No gender difference was observed in the cerebral cortex. We conclude that female rats replete their DHA status more readily than males, probably due to a higher expression of liver desaturases. Our results support the hypothesis on hormonal regulation of PUFA metabolism, which should be taken into account for specific nutritional recommendations.

Authors+Show Affiliations

Unit of Lipid Nutrition and Regulation of Brain Functions, NuRéLiCe, UR909, INRA, F 78352 Jouy-en-Josas, France. audrey.extier@jouy.inra.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19157821

Citation

Extier, Audrey, et al. "Gender Affects Liver Desaturase Expression in a Rat Model of N-3 Fatty Acid Repletion." The Journal of Nutritional Biochemistry, vol. 21, no. 3, 2010, pp. 180-7.
Extier A, Langelier B, Perruchot MH, et al. Gender affects liver desaturase expression in a rat model of n-3 fatty acid repletion. J Nutr Biochem. 2010;21(3):180-7.
Extier, A., Langelier, B., Perruchot, M. H., Guesnet, P., Van Veldhoven, P. P., Lavialle, M., & Alessandri, J. M. (2010). Gender affects liver desaturase expression in a rat model of n-3 fatty acid repletion. The Journal of Nutritional Biochemistry, 21(3), 180-7. https://doi.org/10.1016/j.jnutbio.2008.10.008
Extier A, et al. Gender Affects Liver Desaturase Expression in a Rat Model of N-3 Fatty Acid Repletion. J Nutr Biochem. 2010;21(3):180-7. PubMed PMID: 19157821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gender affects liver desaturase expression in a rat model of n-3 fatty acid repletion. AU - Extier,Audrey, AU - Langelier,Bénédicte, AU - Perruchot,Marie-Hélène, AU - Guesnet,Philippe, AU - Van Veldhoven,Paul P, AU - Lavialle,Monique, AU - Alessandri,Jean-Marc, Y1 - 2009/01/20/ PY - 2008/07/08/received PY - 2008/09/22/revised PY - 2008/10/16/accepted PY - 2009/1/23/entrez PY - 2009/1/23/pubmed PY - 2010/5/7/medline SP - 180 EP - 7 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 21 IS - 3 N2 - Dietary n-3 polyunsaturated fatty acids (PUFA) are major components of cell membranes and have beneficial effects on human health. Docosahexaenoic acid (DHA; 22:6n-3) is the most biologically important n-3 PUFA and can be synthesized from its dietary essential precursor, alpha-linolenic acid (ALA; 18:3n-3). Gender differences in the efficiency of DHA bioconversion have been reported, but underlying molecular mechanisms are unknown. We compared the capacity for DHA synthesis from ALA and the expression of related enzymes in the liver and cerebral cortex between male and female rats. Wistar rats, born with a low-DHA status, were supplied with a suboptimal amount of ALA from weaning to 8 weeks of age. Fatty acid composition was determined by gas chromatography, the mRNA expression of different genes involved in PUFA metabolism was determined by RT-PCR (low-density array) and the expression of proteins was determined by Western blot analysis. At 8 weeks, DHA content was higher (+20 to +40%) in each phospholipid class of female livers compared to male livers. The "Delta4," Delta5 and Delta6 desaturation indexes were 1.2-3 times higher in females than in males. The mRNA expression of Delta5- and Delta6-desaturase genes was 3.8 and 2.5 times greater, respectively, and the Delta5-desaturase protein was higher in female livers (+50%). No gender difference was observed in the cerebral cortex. We conclude that female rats replete their DHA status more readily than males, probably due to a higher expression of liver desaturases. Our results support the hypothesis on hormonal regulation of PUFA metabolism, which should be taken into account for specific nutritional recommendations. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/19157821/Gender_affects_liver_desaturase_expression_in_a_rat_model_of_n_3_fatty_acid_repletion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(08)00244-1 DB - PRIME DP - Unbound Medicine ER -