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Functional TRP and ASIC-like channels in cultured urothelial cells from the rat.
Am J Physiol Renal Physiol. 2009 Apr; 296(4):F892-901.AJ

Abstract

Transient receptor potential (TRP) and acid-sensing ion channels (ASIC) are molecular detectors of chemical, mechanical, thermal, and nociceptive stimuli in sensory neurons. They have been identified in the urothelium, a tissue considered part of bladder sensory pathways, where they might play a role in bladder function. This study investigated functional properties of TRP and ASIC channels in cultured urothelial cells from the rat using patch-clamp and fura 2 Ca(2+) imaging techniques. The TRPV4 agonist 4alpha-phorbol-12,13 didecanoate (4alpha-PDD; 1-5 microM) and the TRPA1/TRPM8 agonist icilin (50-100 microM) elicited transient currents in a high percentage of cells (>70%). 4alpha-PDD responses were suppressed by the TRPV4 antagonist HC-010961 (10 microM). The TRPV1 agonist capsaicin (1-100 microM) and the TRPA1/TRPM8 agonist menthol (5-200 microM) elicited transient currents in a moderate percentage of cells (approximately 25%). All of these agonists increased intracellular calcium concentration ([Ca(2+)](i)). Most cells responded to more than one TRP agonist (e.g., capsaicin and 4alpha-PDD), indicating coexpression of different TRP channels. In the presence of the TRPV1 antagonist capsazepine (10 microM), changes in pH induced by HCl elicited ionic currents (pH 5.5) and increased [Ca(2+)](i) (pH 6.5) in approximately 50% of cells. Changes in pH using acetic acid (pH 5.5) elicited biphasic-like currents. Responses induced by acid were sensitive to amiloride (10 microM). In summary, urothelial cells express multiple TRP and ASIC channels, whose activation elicits ionic currents and Ca(2+) influx. These "neuron-like" properties might be involved in transmitter release, such as ATP, that can act on afferent nerves or smooth muscle to modulate their responses to different stimuli.

Authors+Show Affiliations

Dept. of Pharmacology and Chemical Biology, Univ. of Pittsburgh School of Medicine, E 1340 Biomedical Science Tower, Pittsburgh, PA 15261, USA. flnst2@pitt.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19158342

Citation

Kullmann, F Aura, et al. "Functional TRP and ASIC-like Channels in Cultured Urothelial Cells From the Rat." American Journal of Physiology. Renal Physiology, vol. 296, no. 4, 2009, pp. F892-901.
Kullmann FA, Shah MA, Birder LA, et al. Functional TRP and ASIC-like channels in cultured urothelial cells from the rat. Am J Physiol Renal Physiol. 2009;296(4):F892-901.
Kullmann, F. A., Shah, M. A., Birder, L. A., & de Groat, W. C. (2009). Functional TRP and ASIC-like channels in cultured urothelial cells from the rat. American Journal of Physiology. Renal Physiology, 296(4), F892-901. https://doi.org/10.1152/ajprenal.90718.2008
Kullmann FA, et al. Functional TRP and ASIC-like Channels in Cultured Urothelial Cells From the Rat. Am J Physiol Renal Physiol. 2009;296(4):F892-901. PubMed PMID: 19158342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional TRP and ASIC-like channels in cultured urothelial cells from the rat. AU - Kullmann,F Aura, AU - Shah,M A, AU - Birder,L A, AU - de Groat,W C, Y1 - 2009/01/21/ PY - 2009/1/23/entrez PY - 2009/1/23/pubmed PY - 2009/6/3/medline SP - F892 EP - 901 JF - American journal of physiology. Renal physiology JO - Am J Physiol Renal Physiol VL - 296 IS - 4 N2 - Transient receptor potential (TRP) and acid-sensing ion channels (ASIC) are molecular detectors of chemical, mechanical, thermal, and nociceptive stimuli in sensory neurons. They have been identified in the urothelium, a tissue considered part of bladder sensory pathways, where they might play a role in bladder function. This study investigated functional properties of TRP and ASIC channels in cultured urothelial cells from the rat using patch-clamp and fura 2 Ca(2+) imaging techniques. The TRPV4 agonist 4alpha-phorbol-12,13 didecanoate (4alpha-PDD; 1-5 microM) and the TRPA1/TRPM8 agonist icilin (50-100 microM) elicited transient currents in a high percentage of cells (>70%). 4alpha-PDD responses were suppressed by the TRPV4 antagonist HC-010961 (10 microM). The TRPV1 agonist capsaicin (1-100 microM) and the TRPA1/TRPM8 agonist menthol (5-200 microM) elicited transient currents in a moderate percentage of cells (approximately 25%). All of these agonists increased intracellular calcium concentration ([Ca(2+)](i)). Most cells responded to more than one TRP agonist (e.g., capsaicin and 4alpha-PDD), indicating coexpression of different TRP channels. In the presence of the TRPV1 antagonist capsazepine (10 microM), changes in pH induced by HCl elicited ionic currents (pH 5.5) and increased [Ca(2+)](i) (pH 6.5) in approximately 50% of cells. Changes in pH using acetic acid (pH 5.5) elicited biphasic-like currents. Responses induced by acid were sensitive to amiloride (10 microM). In summary, urothelial cells express multiple TRP and ASIC channels, whose activation elicits ionic currents and Ca(2+) influx. These "neuron-like" properties might be involved in transmitter release, such as ATP, that can act on afferent nerves or smooth muscle to modulate their responses to different stimuli. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/19158342/Functional_TRP_and_ASIC_like_channels_in_cultured_urothelial_cells_from_the_rat_ L2 - https://journals.physiology.org/doi/10.1152/ajprenal.90718.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -