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Electrochemical immunosensing using micro and nanoparticles.
Methods Mol Biol. 2009; 504:145-55.MM

Abstract

A model immunosensor based on a labeling method using gold nanoparticles (AuNPs) and electrochemical detection is developed. Microparamagnetic beads (MB) as primary antibody immobilization platforms and AuNPs modified with a secondary antibody as high sensible electrochemical labels have been used. The carbon electrode used as transducer incorporates a magnet that allows the collection/ immobilization on its surface of the immunological sandwich attached to the MB. Briefly, the sandwich type assay consists in the incubation of streptavidin-coupled-MB with an antihuman IgG biotin conjugate, and then, the immunological reaction with the human IgG antigen takes place. After that, a gold labeled anti-human IgG reacts with the antigen, and finally the AuNPs are electrochemically detected. This approach allows the obtaining of an immunosensor with a low antigen detection limit with special interest for several applications in protein analysis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

de la Escosura-Muñiz A
Nanobioelectronics & Biosensors Group, Institut Català de Nanotecnologia, Barcelona, Catalonia, Spain.
Ambrosi A
No affiliation info available
Alegret S
No affiliation info available
Merkoçi A
No affiliation info available

MeSH

Biosensing TechniquesElectrochemistryEquipment DesignEquipment Failure AnalysisImmunoassayImmunoglobulin GMicroelectrodesMicrospheresNanoparticlesNanotechnologyReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19159096

Citation

de la Escosura-Muñiz, Alfredo, et al. "Electrochemical Immunosensing Using Micro and Nanoparticles." Methods in Molecular Biology (Clifton, N.J.), vol. 504, 2009, pp. 145-55.
de la Escosura-Muñiz A, Ambrosi A, Alegret S, et al. Electrochemical immunosensing using micro and nanoparticles. Methods Mol Biol. 2009;504:145-55.
de la Escosura-Muñiz, A., Ambrosi, A., Alegret, S., & Merkoçi, A. (2009). Electrochemical immunosensing using micro and nanoparticles. Methods in Molecular Biology (Clifton, N.J.), 504, 145-55. https://doi.org/10.1007/978-1-60327-569-9_10
de la Escosura-Muñiz A, et al. Electrochemical Immunosensing Using Micro and Nanoparticles. Methods Mol Biol. 2009;504:145-55. PubMed PMID: 19159096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical immunosensing using micro and nanoparticles. AU - de la Escosura-Muñiz,Alfredo, AU - Ambrosi,Adriano, AU - Alegret,Salvador, AU - Merkoçi,Arben, PY - 2009/1/23/entrez PY - 2009/1/23/pubmed PY - 2009/3/20/medline SP - 145 EP - 55 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 504 N2 - A model immunosensor based on a labeling method using gold nanoparticles (AuNPs) and electrochemical detection is developed. Microparamagnetic beads (MB) as primary antibody immobilization platforms and AuNPs modified with a secondary antibody as high sensible electrochemical labels have been used. The carbon electrode used as transducer incorporates a magnet that allows the collection/ immobilization on its surface of the immunological sandwich attached to the MB. Briefly, the sandwich type assay consists in the incubation of streptavidin-coupled-MB with an antihuman IgG biotin conjugate, and then, the immunological reaction with the human IgG antigen takes place. After that, a gold labeled anti-human IgG reacts with the antigen, and finally the AuNPs are electrochemically detected. This approach allows the obtaining of an immunosensor with a low antigen detection limit with special interest for several applications in protein analysis. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19159096/Electrochemical_immunosensing_using_micro_and_nanoparticles_ L2 - https://dx.doi.org/10.1007/978-1-60327-569-9_10 DB - PRIME DP - Unbound Medicine ER -