Tags

Type your tag names separated by a space and hit enter

Pore dilation occurs in TRPA1 but not in TRPM8 channels.
Mol Pain. 2009 Jan 21; 5:3.MP

Abstract

Abundantly expressed in pain-sensing neurons, TRPV1, TRPA1 and TRPM8 are major cellular sensors of thermal, chemical and mechanical stimuli. The function of these ion channels has been attributed to their selective permeation of small cations (e.g., Ca2+, Na+ and K+), and the ion selectivity has been assumed to be an invariant fingerprint to a given channel. However, for TRPV1, the notion of invariant ion selectivity has been revised recently. When activated, TRPV1 undergoes time and agonist-dependent pore dilation, allowing permeation of large organic cations such as Yo-Pro and NMDG+. The pore dilation is of physiological importance, and has been exploited to specifically silence TRPV1-positive sensory neurons. It is unknown whether TRPA1 and TRPM8 undergo pore dilation. Here we show that TRPA1 activation by reactive or non-reactive agonists induces Yo-Pro uptake, which can be blocked by TRPA1 antagonists. In outside-out patch recordings using NMDG+ as the sole external cation and Na+ as the internal cation, TRPA1 activation results in dynamic changes in permeability to NMDG+. In contrast, TRPM8 activation does not produce either Yo-Pro uptake or significant change in ion selectivity. Hence, pore dilation occurs in TRPA1, but not in TRPM8 channels.

Authors+Show Affiliations

Neuroscience, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064-6125, USA. jun.x.chen@abbott.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19159452

Citation

Chen, Jun, et al. "Pore Dilation Occurs in TRPA1 but Not in TRPM8 Channels." Molecular Pain, vol. 5, 2009, p. 3.
Chen J, Kim D, Bianchi BR, et al. Pore dilation occurs in TRPA1 but not in TRPM8 channels. Mol Pain. 2009;5:3.
Chen, J., Kim, D., Bianchi, B. R., Cavanaugh, E. J., Faltynek, C. R., Kym, P. R., & Reilly, R. M. (2009). Pore dilation occurs in TRPA1 but not in TRPM8 channels. Molecular Pain, 5, 3. https://doi.org/10.1186/1744-8069-5-3
Chen J, et al. Pore Dilation Occurs in TRPA1 but Not in TRPM8 Channels. Mol Pain. 2009 Jan 21;5:3. PubMed PMID: 19159452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pore dilation occurs in TRPA1 but not in TRPM8 channels. AU - Chen,Jun, AU - Kim,Donghee, AU - Bianchi,Bruce R, AU - Cavanaugh,Eric J, AU - Faltynek,Connie R, AU - Kym,Philip R, AU - Reilly,Regina M, Y1 - 2009/01/21/ PY - 2008/10/20/received PY - 2009/01/21/accepted PY - 2009/1/23/entrez PY - 2009/1/23/pubmed PY - 2009/2/13/medline SP - 3 EP - 3 JF - Molecular pain JO - Mol Pain VL - 5 N2 - Abundantly expressed in pain-sensing neurons, TRPV1, TRPA1 and TRPM8 are major cellular sensors of thermal, chemical and mechanical stimuli. The function of these ion channels has been attributed to their selective permeation of small cations (e.g., Ca2+, Na+ and K+), and the ion selectivity has been assumed to be an invariant fingerprint to a given channel. However, for TRPV1, the notion of invariant ion selectivity has been revised recently. When activated, TRPV1 undergoes time and agonist-dependent pore dilation, allowing permeation of large organic cations such as Yo-Pro and NMDG+. The pore dilation is of physiological importance, and has been exploited to specifically silence TRPV1-positive sensory neurons. It is unknown whether TRPA1 and TRPM8 undergo pore dilation. Here we show that TRPA1 activation by reactive or non-reactive agonists induces Yo-Pro uptake, which can be blocked by TRPA1 antagonists. In outside-out patch recordings using NMDG+ as the sole external cation and Na+ as the internal cation, TRPA1 activation results in dynamic changes in permeability to NMDG+. In contrast, TRPM8 activation does not produce either Yo-Pro uptake or significant change in ion selectivity. Hence, pore dilation occurs in TRPA1, but not in TRPM8 channels. SN - 1744-8069 UR - https://www.unboundmedicine.com/medline/citation/19159452/Pore_dilation_occurs_in_TRPA1_but_not_in_TRPM8_channels_ L2 - https://journals.sagepub.com/doi/10.1186/1744-8069-5-3?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -