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Involvement of capsaicin-sensitive afferents and the Transient Receptor Potential Vanilloid 1 Receptor in xylene-induced nocifensive behaviour and inflammation in the mouse.
Neurosci Lett. 2009 Feb 27; 451(3):204-7.NL

Abstract

The inflammatory actions of xylene, an aromatic irritant and sensitizing agent, were described to be predominantly neurogenic in the rat, but the mechanism and the role of the Transient Receptor Potential Vanilloid 1 (TRPV1) capsaicin receptor localized on a subpopulation of sensory nerves has not been elucidated. This paper characterizes the involvement of capsaicin-sensitive afferents and the TRPV1 receptor in nociceptive and acute inflammatory effects of xylene in the mouse. Topical application of xylene on the paw induced a short, intensive nocifensive behaviour characterized by paw liftings and shakings, which was more intensive in Balb/c than in C57Bl/6 mice. Genetic deletion of the TRPV1 receptor as well as destroying capsaicin-sensitive nerve terminals with resiniferatoxin (RTX) pretreatment markedly reduced, but did not abolish nocifensive behaviours. In respect to the xylene-induced plasma protein extravasation detected by Evans blue leakage, significant difference was neither observed between the Balb/c and C57Bl/6 strains, nor the ear and the dorsal paw skin. These inflammatory responses were diminished in the RTX pretreated group, but not in the TRPV1 gene-deleted one. Injection of the antioxidant N-acetylcysteine 15min prior to xylene smearing significantly reduced plasma protein extravasation at both sites. These results demonstrate that xylene-induced acute nocifensive behaviour is mediated by capsaicin-sensitive afferents via TRPV1 receptor activation in mice. Neurogenic inflammatory components play an important role in xylene-induced plasma protein extravasation, but independently of the TRPV1 ion channel. Reactive oxygen or carbonyl species participate in this process presumably via stimulation of the TRPA1 channel.

Authors+Show Affiliations

Department of Pharmacology and Pharmacotherapy, University of Pécs, Szigeti u. 12., Hungary.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19159661

Citation

Sándor, Katalin, et al. "Involvement of Capsaicin-sensitive Afferents and the Transient Receptor Potential Vanilloid 1 Receptor in Xylene-induced Nocifensive Behaviour and Inflammation in the Mouse." Neuroscience Letters, vol. 451, no. 3, 2009, pp. 204-7.
Sándor K, Helyes Z, Elekes K, et al. Involvement of capsaicin-sensitive afferents and the Transient Receptor Potential Vanilloid 1 Receptor in xylene-induced nocifensive behaviour and inflammation in the mouse. Neurosci Lett. 2009;451(3):204-7.
Sándor, K., Helyes, Z., Elekes, K., & Szolcsányi, J. (2009). Involvement of capsaicin-sensitive afferents and the Transient Receptor Potential Vanilloid 1 Receptor in xylene-induced nocifensive behaviour and inflammation in the mouse. Neuroscience Letters, 451(3), 204-7. https://doi.org/10.1016/j.neulet.2009.01.016
Sándor K, et al. Involvement of Capsaicin-sensitive Afferents and the Transient Receptor Potential Vanilloid 1 Receptor in Xylene-induced Nocifensive Behaviour and Inflammation in the Mouse. Neurosci Lett. 2009 Feb 27;451(3):204-7. PubMed PMID: 19159661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of capsaicin-sensitive afferents and the Transient Receptor Potential Vanilloid 1 Receptor in xylene-induced nocifensive behaviour and inflammation in the mouse. AU - Sándor,Katalin, AU - Helyes,Zsuzsanna, AU - Elekes,Krisztián, AU - Szolcsányi,János, Y1 - 2009/01/13/ PY - 2008/10/13/received PY - 2009/01/05/revised PY - 2009/01/07/accepted PY - 2009/1/23/entrez PY - 2009/1/23/pubmed PY - 2009/5/21/medline SP - 204 EP - 7 JF - Neuroscience letters JO - Neurosci Lett VL - 451 IS - 3 N2 - The inflammatory actions of xylene, an aromatic irritant and sensitizing agent, were described to be predominantly neurogenic in the rat, but the mechanism and the role of the Transient Receptor Potential Vanilloid 1 (TRPV1) capsaicin receptor localized on a subpopulation of sensory nerves has not been elucidated. This paper characterizes the involvement of capsaicin-sensitive afferents and the TRPV1 receptor in nociceptive and acute inflammatory effects of xylene in the mouse. Topical application of xylene on the paw induced a short, intensive nocifensive behaviour characterized by paw liftings and shakings, which was more intensive in Balb/c than in C57Bl/6 mice. Genetic deletion of the TRPV1 receptor as well as destroying capsaicin-sensitive nerve terminals with resiniferatoxin (RTX) pretreatment markedly reduced, but did not abolish nocifensive behaviours. In respect to the xylene-induced plasma protein extravasation detected by Evans blue leakage, significant difference was neither observed between the Balb/c and C57Bl/6 strains, nor the ear and the dorsal paw skin. These inflammatory responses were diminished in the RTX pretreated group, but not in the TRPV1 gene-deleted one. Injection of the antioxidant N-acetylcysteine 15min prior to xylene smearing significantly reduced plasma protein extravasation at both sites. These results demonstrate that xylene-induced acute nocifensive behaviour is mediated by capsaicin-sensitive afferents via TRPV1 receptor activation in mice. Neurogenic inflammatory components play an important role in xylene-induced plasma protein extravasation, but independently of the TRPV1 ion channel. Reactive oxygen or carbonyl species participate in this process presumably via stimulation of the TRPA1 channel. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/19159661/Involvement_of_capsaicin_sensitive_afferents_and_the_Transient_Receptor_Potential_Vanilloid_1_Receptor_in_xylene_induced_nocifensive_behaviour_and_inflammation_in_the_mouse_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(09)00043-3 DB - PRIME DP - Unbound Medicine ER -