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Eugenia jambolana Lam. berry extract inhibits growth and induces apoptosis of human breast cancer but not non-tumorigenic breast cells.
J Agric Food Chem. 2009 Feb 11; 57(3):826-31.JA

Abstract

The ripe purple berries of the native Indian plant Eugenia jambolana Lam., known as Jamun, are popularly consumed and available in the United States in Florida and Hawaii. Despite the growing body of data on the chemopreventive potential of edible berry extracts, there is paucity of such data for Jamun fruit. Therefore our laboratory initiated the current study with the following objectives: (1) to prepare a standardized Jamun fruit extract (JFE) for biological studies and (2) to investigate the antiproliferative and pro-apoptotic effects of JFE in estrogen dependent/aromatase positive (MCF-7aro), and estrogen independent (MDA-MB-231) breast cancer cells, and in a normal/nontumorigenic (MCF-10A) breast cell line. JFE was standardized to anthocyanin content using the pH differential method, and individual anthocyanins were identified by high performance liquid chromatography with ultraviolet (HPLC-UV) and tandem mass spectrometry (LC-MS/MS) methods. JFE contained 3.5% anthocyanins (as cyanidin-3-glucoside equivalents) which occur as diglucosides of five anthocyanidins/aglycons: delphinidin, cyanidin, petunidin, peonidin and malvidin. In the proliferation assay, JFE was most effective against MCF-7aro (IC(50) = 27 microg/mL), followed by MDA-MB-231 (IC(50) = 40 microg/mL) breast cancer cells. Importantly, JFE exhibited only mild antiproliferative effects against the normal MCF-10A (IC(50) > 100 microg/mL) breast cells. Similarly, JFE (at 200 microg/mL) exhibited pro-apoptotic effects against the MCF-7aro (p <or= 0.05) and the MDA-MB-231 (p <or= 0.01) breast cancer cells, but not toward the normal MCF-10A breast cells. These studies suggest that JFE may have potential beneficial effects against breast cancer.

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Authors+Show Affiliations

Li L
Department of Biomedical and Pharmaceutical Sciences, Bioactive Botanical Research Laboratory, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island, USA.
Adams LS
No affiliation info available
Chen S
No affiliation info available
Killian C
No affiliation info available
Ahmed A
No affiliation info available
Seeram NP
No affiliation info available

MeSH

AnthocyaninsApoptosisBreast NeoplasmsCell DivisionCell Line, TumorChromatography, High Pressure LiquidFruitHumansPlant ExtractsSyzygium

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19166352

Citation

Li, Liya, et al. "Eugenia Jambolana Lam. Berry Extract Inhibits Growth and Induces Apoptosis of Human Breast Cancer but Not Non-tumorigenic Breast Cells." Journal of Agricultural and Food Chemistry, vol. 57, no. 3, 2009, pp. 826-31.
Li L, Adams LS, Chen S, et al. Eugenia jambolana Lam. berry extract inhibits growth and induces apoptosis of human breast cancer but not non-tumorigenic breast cells. J Agric Food Chem. 2009;57(3):826-31.
Li, L., Adams, L. S., Chen, S., Killian, C., Ahmed, A., & Seeram, N. P. (2009). Eugenia jambolana Lam. berry extract inhibits growth and induces apoptosis of human breast cancer but not non-tumorigenic breast cells. Journal of Agricultural and Food Chemistry, 57(3), 826-31. https://doi.org/10.1021/jf803407q
Li L, et al. Eugenia Jambolana Lam. Berry Extract Inhibits Growth and Induces Apoptosis of Human Breast Cancer but Not Non-tumorigenic Breast Cells. J Agric Food Chem. 2009 Feb 11;57(3):826-31. PubMed PMID: 19166352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eugenia jambolana Lam. berry extract inhibits growth and induces apoptosis of human breast cancer but not non-tumorigenic breast cells. AU - Li,Liya, AU - Adams,Lynn S, AU - Chen,Shiuan, AU - Killian,Caroline, AU - Ahmed,Aftab, AU - Seeram,Navindra P, PY - 2009/1/27/entrez PY - 2009/1/27/pubmed PY - 2009/3/3/medline SP - 826 EP - 31 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 3 N2 - The ripe purple berries of the native Indian plant Eugenia jambolana Lam., known as Jamun, are popularly consumed and available in the United States in Florida and Hawaii. Despite the growing body of data on the chemopreventive potential of edible berry extracts, there is paucity of such data for Jamun fruit. Therefore our laboratory initiated the current study with the following objectives: (1) to prepare a standardized Jamun fruit extract (JFE) for biological studies and (2) to investigate the antiproliferative and pro-apoptotic effects of JFE in estrogen dependent/aromatase positive (MCF-7aro), and estrogen independent (MDA-MB-231) breast cancer cells, and in a normal/nontumorigenic (MCF-10A) breast cell line. JFE was standardized to anthocyanin content using the pH differential method, and individual anthocyanins were identified by high performance liquid chromatography with ultraviolet (HPLC-UV) and tandem mass spectrometry (LC-MS/MS) methods. JFE contained 3.5% anthocyanins (as cyanidin-3-glucoside equivalents) which occur as diglucosides of five anthocyanidins/aglycons: delphinidin, cyanidin, petunidin, peonidin and malvidin. In the proliferation assay, JFE was most effective against MCF-7aro (IC(50) = 27 microg/mL), followed by MDA-MB-231 (IC(50) = 40 microg/mL) breast cancer cells. Importantly, JFE exhibited only mild antiproliferative effects against the normal MCF-10A (IC(50) > 100 microg/mL) breast cells. Similarly, JFE (at 200 microg/mL) exhibited pro-apoptotic effects against the MCF-7aro (p <or= 0.05) and the MDA-MB-231 (p <or= 0.01) breast cancer cells, but not toward the normal MCF-10A breast cells. These studies suggest that JFE may have potential beneficial effects against breast cancer. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19166352/Eugenia_jambolana_Lam__berry_extract_inhibits_growth_and_induces_apoptosis_of_human_breast_cancer_but_not_non_tumorigenic_breast_cells_ L2 - https://doi.org/10.1021/jf803407q DB - PRIME DP - Unbound Medicine ER -