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Comprehensive two-dimensional gas chromatography for fingerprint pattern recognition in olive oils produced by two different techniques in Portuguese olive varieties Galega Vulgar, Cobrançosa e Carrasquenha.
Anal Chim Acta. 2009 Feb 09; 633(2):263-70.AC

Abstract

For olive oil production a metal hammer-decanter olive processing line was compared to a traditional metal hammer-press line, a discontinuous method which, if properly used, yields high-quality virgin olive oils. Galega, Carrasquenha and Cobrançosa olives (traditional Portuguese varieties) were studied. The analysis of the aroma compounds was performed after headspace-solid phase micro extraction. The analytical results obtained after comprehensive gas chromatography in tandem with time of flight mass spectrometry (GCxGC/ToFMS) for these three different olive oil varieties, from a single year harvest and processed with two different extraction technologies, were compared using statistical image treatment, by means of ImageJ software, for fingerprint recognitions and compared with principal component analysis when the area data of each chromatographic spot of the contour plots were considered. The differences used to classify the olive oils studied under different groups after principal component analysis were observed independently of the treatment used (peak areas or the sum of the pixels counts). When the individual peak areas were considered, more then 75.7% of the total variance is explained by the first two principal components while in the case where the data were subjected to image treatment 84.0% of the total variance is explained by the first two principal components. In both cases the first and second principal components present eigenvalues higher then 1.0. Fingerprint image monitoring of the aroma compounds of the olive oil allowed a rapid differentiation of the three varieties studied as well as the extraction methods used. The volatile compounds responsible for their characterization were tentatively identified in a bi-dimensional polar/non-polar column set in the GCxGC/Tof-MS apparatus. This methodology allowed the reduction of the number of compounds needed for matrices characterization, preserving the efficiency of the discrimination, when compared with the traditional methods where the identification of all peaks is needed.

Authors+Show Affiliations

ICAM (Instituto de Ciências Agrárias e Mediterrânicas), Departamento de Fitotecnia, Universidade de Evora, Apt 94, 7002-554 Evora, Portugal.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19166732

Citation

Vaz-Freire, L Torres, et al. "Comprehensive Two-dimensional Gas Chromatography for Fingerprint Pattern Recognition in Olive Oils Produced By Two Different Techniques in Portuguese Olive Varieties Galega Vulgar, Cobrançosa E Carrasquenha." Analytica Chimica Acta, vol. 633, no. 2, 2009, pp. 263-70.
Vaz-Freire LT, da Silva MD, Freitas AM. Comprehensive two-dimensional gas chromatography for fingerprint pattern recognition in olive oils produced by two different techniques in Portuguese olive varieties Galega Vulgar, Cobrançosa e Carrasquenha. Anal Chim Acta. 2009;633(2):263-70.
Vaz-Freire, L. T., da Silva, M. D., & Freitas, A. M. (2009). Comprehensive two-dimensional gas chromatography for fingerprint pattern recognition in olive oils produced by two different techniques in Portuguese olive varieties Galega Vulgar, Cobrançosa e Carrasquenha. Analytica Chimica Acta, 633(2), 263-70. https://doi.org/10.1016/j.aca.2008.11.057
Vaz-Freire LT, da Silva MD, Freitas AM. Comprehensive Two-dimensional Gas Chromatography for Fingerprint Pattern Recognition in Olive Oils Produced By Two Different Techniques in Portuguese Olive Varieties Galega Vulgar, Cobrançosa E Carrasquenha. Anal Chim Acta. 2009 Feb 9;633(2):263-70. PubMed PMID: 19166732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive two-dimensional gas chromatography for fingerprint pattern recognition in olive oils produced by two different techniques in Portuguese olive varieties Galega Vulgar, Cobrançosa e Carrasquenha. AU - Vaz-Freire,L Torres, AU - da Silva,M D R Gomes, AU - Freitas,A M Costa, Y1 - 2008/12/03/ PY - 2008/08/11/received PY - 2008/10/27/revised PY - 2008/11/22/accepted PY - 2009/1/27/entrez PY - 2009/1/27/pubmed PY - 2009/3/11/medline SP - 263 EP - 70 JF - Analytica chimica acta JO - Anal Chim Acta VL - 633 IS - 2 N2 - For olive oil production a metal hammer-decanter olive processing line was compared to a traditional metal hammer-press line, a discontinuous method which, if properly used, yields high-quality virgin olive oils. Galega, Carrasquenha and Cobrançosa olives (traditional Portuguese varieties) were studied. The analysis of the aroma compounds was performed after headspace-solid phase micro extraction. The analytical results obtained after comprehensive gas chromatography in tandem with time of flight mass spectrometry (GCxGC/ToFMS) for these three different olive oil varieties, from a single year harvest and processed with two different extraction technologies, were compared using statistical image treatment, by means of ImageJ software, for fingerprint recognitions and compared with principal component analysis when the area data of each chromatographic spot of the contour plots were considered. The differences used to classify the olive oils studied under different groups after principal component analysis were observed independently of the treatment used (peak areas or the sum of the pixels counts). When the individual peak areas were considered, more then 75.7% of the total variance is explained by the first two principal components while in the case where the data were subjected to image treatment 84.0% of the total variance is explained by the first two principal components. In both cases the first and second principal components present eigenvalues higher then 1.0. Fingerprint image monitoring of the aroma compounds of the olive oil allowed a rapid differentiation of the three varieties studied as well as the extraction methods used. The volatile compounds responsible for their characterization were tentatively identified in a bi-dimensional polar/non-polar column set in the GCxGC/Tof-MS apparatus. This methodology allowed the reduction of the number of compounds needed for matrices characterization, preserving the efficiency of the discrimination, when compared with the traditional methods where the identification of all peaks is needed. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/19166732/Comprehensive_two_dimensional_gas_chromatography_for_fingerprint_pattern_recognition_in_olive_oils_produced_by_two_different_techniques_in_Portuguese_olive_varieties_Galega_Vulgar_Cobrançosa_e_Carrasquenha_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(08)01993-4 DB - PRIME DP - Unbound Medicine ER -