Tags

Type your tag names separated by a space and hit enter

Application of ultra-high-pressure liquid chromatography-tandem mass spectrometry to the determination of multi-class pesticides in environmental and wastewater samples. Study of matrix effects.
J Chromatogr A. 2009 Feb 27; 1216(9):1410-20.JC

Abstract

An ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the determination of 37 pesticides (herbicides, insecticides and fungicides) in environmental and wastewater has been developed. To efficiently combine UHPLC with MS/MS, a fast-acquisition triple quadrupole mass analyzer was used. This analyzer (minimum dwell time, 5 ms) allows acquiring up to three simultaneous transitions in the selected reaction monitoring mode for each compound assuring a reliable identification without resolution or sensitivity losses. A pre-concentration step based on solid-phase extraction using Waters Oasis HLB cartridges (0.2 g) was applied with a 100-fold pre-concentration factor along the whole analytical procedure. The method was validated based on European SANCO guidelines using surface, ground, drinking and treated water (from an urban solid residues treatment plant) spiked at two concentration levels (0.025 and 0.1 microg/L), the lowest having been established as the limit of quantification objective. The method showed excellent sensitivity, with instrumental limits of detection ranging from 0.1 to 7 pg. It was applied to environmental water samples (ground and surface water) as well as to samples of urban solid waste leachates (raw leachate and treated leachate after applying reversed osmosis) collected from a municipal treatment plant. Matrix effects have been studied in the different types of water samples analyzed, and several isotope-labelled internal standards have been evaluated as a way to compensate the signal suppression observed for most of the compounds studied, especially in wastewater samples. As a general remark, only those pesticides which response was corrected using their own isotope-labelled molecule, could be satisfactorily corrected in all type of samples, assuring in this way the accurate quantification in all matrix samples.

Authors+Show Affiliations

Research Institute for Pesticides and Water, University Jaume I, Avda. Sos Baynat, E-12071 Castellón, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19167004

Citation

Marín, José M., et al. "Application of Ultra-high-pressure Liquid Chromatography-tandem Mass Spectrometry to the Determination of Multi-class Pesticides in Environmental and Wastewater Samples. Study of Matrix Effects." Journal of Chromatography. A, vol. 1216, no. 9, 2009, pp. 1410-20.
Marín JM, Gracia-Lor E, Sancho JV, et al. Application of ultra-high-pressure liquid chromatography-tandem mass spectrometry to the determination of multi-class pesticides in environmental and wastewater samples. Study of matrix effects. J Chromatogr A. 2009;1216(9):1410-20.
Marín, J. M., Gracia-Lor, E., Sancho, J. V., López, F. J., & Hernández, F. (2009). Application of ultra-high-pressure liquid chromatography-tandem mass spectrometry to the determination of multi-class pesticides in environmental and wastewater samples. Study of matrix effects. Journal of Chromatography. A, 1216(9), 1410-20. https://doi.org/10.1016/j.chroma.2008.12.094
Marín JM, et al. Application of Ultra-high-pressure Liquid Chromatography-tandem Mass Spectrometry to the Determination of Multi-class Pesticides in Environmental and Wastewater Samples. Study of Matrix Effects. J Chromatogr A. 2009 Feb 27;1216(9):1410-20. PubMed PMID: 19167004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of ultra-high-pressure liquid chromatography-tandem mass spectrometry to the determination of multi-class pesticides in environmental and wastewater samples. Study of matrix effects. AU - Marín,José M, AU - Gracia-Lor,Emma, AU - Sancho,Juan V, AU - López,Francisco J, AU - Hernández,Félix, Y1 - 2009/01/08/ PY - 2008/09/17/received PY - 2008/12/16/revised PY - 2008/12/30/accepted PY - 2009/1/27/entrez PY - 2009/1/27/pubmed PY - 2009/5/1/medline SP - 1410 EP - 20 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 9 N2 - An ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the determination of 37 pesticides (herbicides, insecticides and fungicides) in environmental and wastewater has been developed. To efficiently combine UHPLC with MS/MS, a fast-acquisition triple quadrupole mass analyzer was used. This analyzer (minimum dwell time, 5 ms) allows acquiring up to three simultaneous transitions in the selected reaction monitoring mode for each compound assuring a reliable identification without resolution or sensitivity losses. A pre-concentration step based on solid-phase extraction using Waters Oasis HLB cartridges (0.2 g) was applied with a 100-fold pre-concentration factor along the whole analytical procedure. The method was validated based on European SANCO guidelines using surface, ground, drinking and treated water (from an urban solid residues treatment plant) spiked at two concentration levels (0.025 and 0.1 microg/L), the lowest having been established as the limit of quantification objective. The method showed excellent sensitivity, with instrumental limits of detection ranging from 0.1 to 7 pg. It was applied to environmental water samples (ground and surface water) as well as to samples of urban solid waste leachates (raw leachate and treated leachate after applying reversed osmosis) collected from a municipal treatment plant. Matrix effects have been studied in the different types of water samples analyzed, and several isotope-labelled internal standards have been evaluated as a way to compensate the signal suppression observed for most of the compounds studied, especially in wastewater samples. As a general remark, only those pesticides which response was corrected using their own isotope-labelled molecule, could be satisfactorily corrected in all type of samples, assuring in this way the accurate quantification in all matrix samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19167004/Application_of_ultra_high_pressure_liquid_chromatography_tandem_mass_spectrometry_to_the_determination_of_multi_class_pesticides_in_environmental_and_wastewater_samples__Study_of_matrix_effects_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00016-8 DB - PRIME DP - Unbound Medicine ER -