Abstract
In this paper, we report the use of micellar electrokinetic chromatography (MEKC) for the highly efficient preconcentration and separation of gold nanoparticles (Au NPs). We used the reversed electrode polarity stacking mode (REPSM) of the MEKC system for the on-line enhancement and separation of the Au NPs. Several parameters had dramatic effects on the systems' performance, including the concentration of sodium dodecylsulfate (SDS) surfactant, the presence of salts in the NP solution, the pH of the running electrolyte, and the temperature of the capillary. Under the optimized conditions [buffer: SDS (70 mM) and 3-cyclohexylamino-1-propanesulfonic acid (CAPS; 10 mM) at pH 10.0; applied voltage: 20 kV; operating temperature: 25 degrees C; additive: sodium dihydrogenphosphate (NaH(2)PO(4), 10 mM); REPSM strategy for sample preconcentration], the number of theoretical plates for the 5.3- and 40.1-nm-diameter Au NPs were 3000 and (an ultrahigh) 2.1 x 10(6), respectively; in addition, the detection sensitivities toward the Au NPs were enhanced ca. 20- and 380-fold, respectively, relative to those obtained using standard MEKC analysis conditions. Furthermore, monitoring the electropherograms using diode-array detection allowed us to identify and characterize the sizes of the separated NPs from their UV-vis spectra. Our findings suggest that MEKC is a highly efficient tool for both the preconcentration and separation of NPs.
TY - JOUR
T1 - Using micellar electrokinetic chromatography for the highly efficient preconcentration and separation of gold nanoparticles.
A1 - Liu,Fu-Ken,
Y1 - 2009/01/09/
PY - 2008/09/05/received
PY - 2008/12/19/revised
PY - 2009/01/05/accepted
PY - 2009/1/27/entrez
PY - 2009/1/27/pubmed
PY - 2009/8/14/medline
SP - 2554
EP - 9
JF - Journal of chromatography. A
JO - J Chromatogr A
VL - 1216
IS - 12
N2 - In this paper, we report the use of micellar electrokinetic chromatography (MEKC) for the highly efficient preconcentration and separation of gold nanoparticles (Au NPs). We used the reversed electrode polarity stacking mode (REPSM) of the MEKC system for the on-line enhancement and separation of the Au NPs. Several parameters had dramatic effects on the systems' performance, including the concentration of sodium dodecylsulfate (SDS) surfactant, the presence of salts in the NP solution, the pH of the running electrolyte, and the temperature of the capillary. Under the optimized conditions [buffer: SDS (70 mM) and 3-cyclohexylamino-1-propanesulfonic acid (CAPS; 10 mM) at pH 10.0; applied voltage: 20 kV; operating temperature: 25 degrees C; additive: sodium dihydrogenphosphate (NaH(2)PO(4), 10 mM); REPSM strategy for sample preconcentration], the number of theoretical plates for the 5.3- and 40.1-nm-diameter Au NPs were 3000 and (an ultrahigh) 2.1 x 10(6), respectively; in addition, the detection sensitivities toward the Au NPs were enhanced ca. 20- and 380-fold, respectively, relative to those obtained using standard MEKC analysis conditions. Furthermore, monitoring the electropherograms using diode-array detection allowed us to identify and characterize the sizes of the separated NPs from their UV-vis spectra. Our findings suggest that MEKC is a highly efficient tool for both the preconcentration and separation of NPs.
SN - 1873-3778
UR - https://www.unboundmedicine.com/medline/citation/19167005/Using_micellar_electrokinetic_chromatography_for_the_highly_efficient_preconcentration_and_separation_of_gold_nanoparticles_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00025-9
DB - PRIME
DP - Unbound Medicine
ER -
