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Chemopreventive action of Lygodium flexuosum extract in human hepatoma PLC/PRF/5 and Hep 3B cells.
J Ethnopharmacol. 2009 Mar 18; 122(2):294-303.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Lygodium flexuosum (Lygodiaceae), a medicinal fern used in Indian traditional medicine against liver disorders.

AIM OF THE STUDY

The rationale of the study was to examine whether the n-hexane extract from plant Lygodium flexuosum affects apoptosis on human hepatoma PLC/PRF/5 and Hep 3B cells.

MATERIALS AND METHODS

Chemopreventive activity of the Lygodium flexuosum extract was determined by MTT assay, annexin-V FITC binding to phosphatidyl serine and cleavage of PARP. Subdiploid condition of cells treated with Lygodium flexuosum was analyzed by flow cytometry. Further, used transiently transfected NF-kappaB reporter in PLC/PRF/5 cells to evaluate the inhibitive effect of Lygodium flexuosum extract.

RESULTS

Lygodium flexuosum extract inhibited the cell viability and induced apoptosis in hepatoma cells in a concentration dependent manner as evidenced by apoptotic changes such as flipping of phosphatidyl serine, cleavage of PARP. Cell cycle analysis showed the subG1 apoptotic population in cells treated with higher concentrations of the extract. When activated with exogenous TNF-alpha in transfected hepatoma cells it was observed that NF-kappaB dependent gene expression was inhibited by treatment with Lygodium flexuosum extract in PLC/PRF/5 cells dose-dependently.

CONCLUSIONS

This investigation suggests that the Lygodium flexuosum extract has antiproliferative and apoptotic activity in both cancer cells and has inhibitive role in TNF-alpha induced NF-kappaB activation in PLC/PRF/5 cells confirms the potential of the extract as a chemopreventive agent.

Authors+Show Affiliations

Molecular Ethnopharmacology Laboratory, Division of Molecular Medicine and Cancer Biology, Rajiv Gandhi Center for Biotechnology, Thiruvananthapuram, Kerala 695014, India. willspj@yahoo.co.inNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19168119

Citation

Wills, P J., and V V. Asha. "Chemopreventive Action of Lygodium Flexuosum Extract in Human Hepatoma PLC/PRF/5 and Hep 3B Cells." Journal of Ethnopharmacology, vol. 122, no. 2, 2009, pp. 294-303.
Wills PJ, Asha VV. Chemopreventive action of Lygodium flexuosum extract in human hepatoma PLC/PRF/5 and Hep 3B cells. J Ethnopharmacol. 2009;122(2):294-303.
Wills, P. J., & Asha, V. V. (2009). Chemopreventive action of Lygodium flexuosum extract in human hepatoma PLC/PRF/5 and Hep 3B cells. Journal of Ethnopharmacology, 122(2), 294-303. https://doi.org/10.1016/j.jep.2009.01.006
Wills PJ, Asha VV. Chemopreventive Action of Lygodium Flexuosum Extract in Human Hepatoma PLC/PRF/5 and Hep 3B Cells. J Ethnopharmacol. 2009 Mar 18;122(2):294-303. PubMed PMID: 19168119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemopreventive action of Lygodium flexuosum extract in human hepatoma PLC/PRF/5 and Hep 3B cells. AU - Wills,P J, AU - Asha,V V, Y1 - 2009/01/09/ PY - 2008/08/30/received PY - 2008/12/01/revised PY - 2009/01/03/accepted PY - 2009/1/27/entrez PY - 2009/1/27/pubmed PY - 2009/5/20/medline SP - 294 EP - 303 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 122 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Lygodium flexuosum (Lygodiaceae), a medicinal fern used in Indian traditional medicine against liver disorders. AIM OF THE STUDY: The rationale of the study was to examine whether the n-hexane extract from plant Lygodium flexuosum affects apoptosis on human hepatoma PLC/PRF/5 and Hep 3B cells. MATERIALS AND METHODS: Chemopreventive activity of the Lygodium flexuosum extract was determined by MTT assay, annexin-V FITC binding to phosphatidyl serine and cleavage of PARP. Subdiploid condition of cells treated with Lygodium flexuosum was analyzed by flow cytometry. Further, used transiently transfected NF-kappaB reporter in PLC/PRF/5 cells to evaluate the inhibitive effect of Lygodium flexuosum extract. RESULTS: Lygodium flexuosum extract inhibited the cell viability and induced apoptosis in hepatoma cells in a concentration dependent manner as evidenced by apoptotic changes such as flipping of phosphatidyl serine, cleavage of PARP. Cell cycle analysis showed the subG1 apoptotic population in cells treated with higher concentrations of the extract. When activated with exogenous TNF-alpha in transfected hepatoma cells it was observed that NF-kappaB dependent gene expression was inhibited by treatment with Lygodium flexuosum extract in PLC/PRF/5 cells dose-dependently. CONCLUSIONS: This investigation suggests that the Lygodium flexuosum extract has antiproliferative and apoptotic activity in both cancer cells and has inhibitive role in TNF-alpha induced NF-kappaB activation in PLC/PRF/5 cells confirms the potential of the extract as a chemopreventive agent. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19168119/Chemopreventive_action_of_Lygodium_flexuosum_extract_in_human_hepatoma_PLC/PRF/5_and_Hep_3B_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00022-1 DB - PRIME DP - Unbound Medicine ER -