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Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA.
Indian J Med Microbiol. 2009 Jan-Mar; 27(1):27-9.IJ

Abstract

Cefoxitin is a potent inducer of the mecA regulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecA gene by PCR.

MATERIALS AND METHODS

Fifty strains of S. aureus isolated from clinical samples were used in the study. Routine antibiotic susceptibility testing was performed including oxacillin disk. Oxacillin screen agar plates with 4% NaCl and 6 microg/ml of oxacillin were inoculated and interpreted as per standard guidelines. Cefoxitin disc diffusion test was performed using 30 microg disc and zone sizes were measured. PCR for amplification of the mecA gene was performed.

RESULTS

Out of the 50 isolates, 28 were found to be methicillin resistant by oxacillin disc diffusion test, 30 were resistant by oxacillin screen agar method, and 32 were resistant with cefoxitin disc diffusion. For these 32 isolates mecA gene was positive.

CONCLUSION

Results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene. Thus, the test can be an alternative to PCR for detection of MRSA in resource constraint settings.

Authors+Show Affiliations

Department of Microbiology, Armed Forces Medical College, Pune-440 040, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

19172055

Citation

Anand, K B., et al. "Comparison of Cefoxitin Disc Diffusion Test, Oxacillin Screen Agar, and PCR for mecA Gene for Detection of MRSA." Indian Journal of Medical Microbiology, vol. 27, no. 1, 2009, pp. 27-9.
Anand KB, Agrawal P, Kumar S, et al. Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Indian J Med Microbiol. 2009;27(1):27-9.
Anand, K. B., Agrawal, P., Kumar, S., & Kapila, K. (2009). Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Indian Journal of Medical Microbiology, 27(1), 27-9.
Anand KB, et al. Comparison of Cefoxitin Disc Diffusion Test, Oxacillin Screen Agar, and PCR for mecA Gene for Detection of MRSA. Indian J Med Microbiol. 2009;27(1):27-9. PubMed PMID: 19172055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. AU - Anand,K B, AU - Agrawal,P, AU - Kumar,S, AU - Kapila,K, PY - 2009/1/28/entrez PY - 2009/1/28/pubmed PY - 2009/3/13/medline SP - 27 EP - 9 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 27 IS - 1 N2 - UNLABELLED: Cefoxitin is a potent inducer of the mecA regulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecA gene by PCR. MATERIALS AND METHODS: Fifty strains of S. aureus isolated from clinical samples were used in the study. Routine antibiotic susceptibility testing was performed including oxacillin disk. Oxacillin screen agar plates with 4% NaCl and 6 microg/ml of oxacillin were inoculated and interpreted as per standard guidelines. Cefoxitin disc diffusion test was performed using 30 microg disc and zone sizes were measured. PCR for amplification of the mecA gene was performed. RESULTS: Out of the 50 isolates, 28 were found to be methicillin resistant by oxacillin disc diffusion test, 30 were resistant by oxacillin screen agar method, and 32 were resistant with cefoxitin disc diffusion. For these 32 isolates mecA gene was positive. CONCLUSION: Results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene. Thus, the test can be an alternative to PCR for detection of MRSA in resource constraint settings. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/19172055/Comparison_of_cefoxitin_disc_diffusion_test_oxacillin_screen_agar_and_PCR_for_mecA_gene_for_detection_of_MRSA_ L2 - http://www.ijmm.org/article.asp?issn=0255-0857;year=2009;volume=27;issue=1;spage=27;epage=29;aulast=Anand DB - PRIME DP - Unbound Medicine ER -