Tags

Type your tag names separated by a space and hit enter

Morphological changes of the enteric nervous system, interstitial cells of cajal, and smooth muscle in children with colonic motility disorders.
J Pediatr Gastroenterol Nutr. 2009 Jan; 48(1):22-9.JP

Abstract

OBJECTIVES

To evaluate the relation between colonic manometry findings and the colonic enteric nervous system, interstitial cells of Cajal, and smooth muscle morphology.

PATIENTS AND METHODS

Colonic specimens from surgical resections or full-thickness biopsy specimens were assessed from a cohort of children who underwent colonic manometry before surgery. Colonic manometric patterns were subdivided into high-amplitude propagating contractions, low-amplitude propagating contractions, absence of contractions, and low-amplitude simultaneous contractions. Immunohistochemistry was performed to identify abnormalities in the enteric nervous system, interstitial cells of Cajal, and smooth muscle layers.

RESULTS

Study participants included patients with Hirschsprung disease (n = 4), chronic intestinal pseudo-obstruction (n = 1), and idiopathic intractable constipation (n = 8). Thirty-seven ganglionic segments were studied. Abnormalities in myenteric plexus were recognized in segments of all manometry groups, and no differences could be identified when they were compared with segments with high-amplitude propagating contractions. All of the segments showed an abnormal interstitial cells of Cajal plexus, and no statistical difference could be identified between the 4 groups (n = 0.08). Homogeneous expression of smooth muscle actin was observed in all of the segments.

CONCLUSIONS

In this cohort we were unable to classify specific manometric findings as reflective of myopathic or neuropathic abnormalities in patients with motility disorders. Caution should be used when predicting the type of neuromuscular disorder based on colonic manometry.

Authors+Show Affiliations

Department of Pediatrics, Division of Gastroenterology, Children's Hospital of Columbus, Ohio State University, Columbus, OH, USA. maartjemvdberg@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19172119

Citation

van den Berg, M M., et al. "Morphological Changes of the Enteric Nervous System, Interstitial Cells of Cajal, and Smooth Muscle in Children With Colonic Motility Disorders." Journal of Pediatric Gastroenterology and Nutrition, vol. 48, no. 1, 2009, pp. 22-9.
van den Berg MM, Di Lorenzo C, Mousa HM, et al. Morphological changes of the enteric nervous system, interstitial cells of cajal, and smooth muscle in children with colonic motility disorders. J Pediatr Gastroenterol Nutr. 2009;48(1):22-9.
van den Berg, M. M., Di Lorenzo, C., Mousa, H. M., Benninga, M. A., Boeckxstaens, G. E., & Luquette, M. (2009). Morphological changes of the enteric nervous system, interstitial cells of cajal, and smooth muscle in children with colonic motility disorders. Journal of Pediatric Gastroenterology and Nutrition, 48(1), 22-9. https://doi.org/10.1097/MPG.0b013e318173293b
van den Berg MM, et al. Morphological Changes of the Enteric Nervous System, Interstitial Cells of Cajal, and Smooth Muscle in Children With Colonic Motility Disorders. J Pediatr Gastroenterol Nutr. 2009;48(1):22-9. PubMed PMID: 19172119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological changes of the enteric nervous system, interstitial cells of cajal, and smooth muscle in children with colonic motility disorders. AU - van den Berg,M M, AU - Di Lorenzo,C, AU - Mousa,H M, AU - Benninga,M A, AU - Boeckxstaens,G E E, AU - Luquette,M, PY - 2009/1/28/entrez PY - 2009/1/28/pubmed PY - 2009/4/7/medline SP - 22 EP - 9 JF - Journal of pediatric gastroenterology and nutrition JO - J Pediatr Gastroenterol Nutr VL - 48 IS - 1 N2 - OBJECTIVES: To evaluate the relation between colonic manometry findings and the colonic enteric nervous system, interstitial cells of Cajal, and smooth muscle morphology. PATIENTS AND METHODS: Colonic specimens from surgical resections or full-thickness biopsy specimens were assessed from a cohort of children who underwent colonic manometry before surgery. Colonic manometric patterns were subdivided into high-amplitude propagating contractions, low-amplitude propagating contractions, absence of contractions, and low-amplitude simultaneous contractions. Immunohistochemistry was performed to identify abnormalities in the enteric nervous system, interstitial cells of Cajal, and smooth muscle layers. RESULTS: Study participants included patients with Hirschsprung disease (n = 4), chronic intestinal pseudo-obstruction (n = 1), and idiopathic intractable constipation (n = 8). Thirty-seven ganglionic segments were studied. Abnormalities in myenteric plexus were recognized in segments of all manometry groups, and no differences could be identified when they were compared with segments with high-amplitude propagating contractions. All of the segments showed an abnormal interstitial cells of Cajal plexus, and no statistical difference could be identified between the 4 groups (n = 0.08). Homogeneous expression of smooth muscle actin was observed in all of the segments. CONCLUSIONS: In this cohort we were unable to classify specific manometric findings as reflective of myopathic or neuropathic abnormalities in patients with motility disorders. Caution should be used when predicting the type of neuromuscular disorder based on colonic manometry. SN - 1536-4801 UR - https://www.unboundmedicine.com/medline/citation/19172119/Morphological_changes_of_the_enteric_nervous_system_interstitial_cells_of_cajal_and_smooth_muscle_in_children_with_colonic_motility_disorders_ L2 - https://doi.org/10.1097/MPG.0b013e318173293b DB - PRIME DP - Unbound Medicine ER -