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Field validity and feasibility of four techniques for the detection of Trichuris in simians: a model for monitoring drug efficacy in public health?
PLoS Negl Trop Dis. 2009; 3(1):e366.PN

Abstract

BACKGROUND

Soil-transmitted helminths, such as Trichuris trichiura, are of major concern in public health. Current efforts to control these helminth infections involve periodic mass treatment in endemic areas. Since these large-scale interventions are likely to intensify, monitoring the drug efficacy will become indispensible. However, studies comparing detection techniques based on sensitivity, fecal egg counts (FEC), feasibility for mass diagnosis and drug efficacy estimates are scarce.

METHODOLOGY/PRINCIPAL FINDINGS

In the present study, the ether-based concentration, the Parasep Solvent Free (SF), the McMaster and the FLOTAC techniques were compared based on both validity and feasibility for the detection of Trichuris eggs in 100 fecal samples of nonhuman primates. In addition, the drug efficacy estimates of quantitative techniques was examined using a statistical simulation. Trichuris eggs were found in 47% of the samples. FLOTAC was the most sensitive technique (100%), followed by the Parasep SF (83.0% [95% confidence interval (CI): 82.4-83.6%]) and the ether-based concentration technique (76.6% [95% CI: 75.8-77.3%]). McMaster was the least sensitive (61.7% [95% CI: 60.7-62.6%]) and failed to detect low FEC. The quantitative comparison revealed a positive correlation between the four techniques (Rs = 0.85-0.93; p<0.0001). However, the ether-based concentration technique and the Parasep SF detected significantly fewer eggs than both the McMaster and the FLOTAC (p<0.0083). Overall, the McMaster was the most feasible technique (3.9 min/sample for preparing, reading and cleaning of the apparatus), followed by the ether-based concentration technique (7.7 min/sample) and the FLOTAC (9.8 min/sample). Parasep SF was the least feasible (17.7 min/sample). The simulation revealed that the sensitivity is less important for monitoring drug efficacy and that both FLOTAC and McMaster were reliable estimators.

CONCLUSIONS/SIGNIFICANCE

The results of this study demonstrated that McMaster is a promising technique when making use of FEC to monitor drug efficacy in Trichuris.

Authors+Show Affiliations

Department of Virology, Parasitology & Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Bruno.Levecke@ugent.beNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19172171

Citation

Levecke, Bruno, et al. "Field Validity and Feasibility of Four Techniques for the Detection of Trichuris in Simians: a Model for Monitoring Drug Efficacy in Public Health?" PLoS Neglected Tropical Diseases, vol. 3, no. 1, 2009, pp. e366.
Levecke B, De Wilde N, Vandenhoute E, et al. Field validity and feasibility of four techniques for the detection of Trichuris in simians: a model for monitoring drug efficacy in public health? PLoS Negl Trop Dis. 2009;3(1):e366.
Levecke, B., De Wilde, N., Vandenhoute, E., & Vercruysse, J. (2009). Field validity and feasibility of four techniques for the detection of Trichuris in simians: a model for monitoring drug efficacy in public health? PLoS Neglected Tropical Diseases, 3(1), e366. https://doi.org/10.1371/journal.pntd.0000366
Levecke B, et al. Field Validity and Feasibility of Four Techniques for the Detection of Trichuris in Simians: a Model for Monitoring Drug Efficacy in Public Health. PLoS Negl Trop Dis. 2009;3(1):e366. PubMed PMID: 19172171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Field validity and feasibility of four techniques for the detection of Trichuris in simians: a model for monitoring drug efficacy in public health? AU - Levecke,Bruno, AU - De Wilde,Nathalie, AU - Vandenhoute,Els, AU - Vercruysse,Jozef, Y1 - 2009/01/27/ PY - 2008/07/01/received PY - 2008/12/17/accepted PY - 2009/1/28/entrez PY - 2009/1/28/pubmed PY - 2010/3/24/medline SP - e366 EP - e366 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 3 IS - 1 N2 - BACKGROUND: Soil-transmitted helminths, such as Trichuris trichiura, are of major concern in public health. Current efforts to control these helminth infections involve periodic mass treatment in endemic areas. Since these large-scale interventions are likely to intensify, monitoring the drug efficacy will become indispensible. However, studies comparing detection techniques based on sensitivity, fecal egg counts (FEC), feasibility for mass diagnosis and drug efficacy estimates are scarce. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, the ether-based concentration, the Parasep Solvent Free (SF), the McMaster and the FLOTAC techniques were compared based on both validity and feasibility for the detection of Trichuris eggs in 100 fecal samples of nonhuman primates. In addition, the drug efficacy estimates of quantitative techniques was examined using a statistical simulation. Trichuris eggs were found in 47% of the samples. FLOTAC was the most sensitive technique (100%), followed by the Parasep SF (83.0% [95% confidence interval (CI): 82.4-83.6%]) and the ether-based concentration technique (76.6% [95% CI: 75.8-77.3%]). McMaster was the least sensitive (61.7% [95% CI: 60.7-62.6%]) and failed to detect low FEC. The quantitative comparison revealed a positive correlation between the four techniques (Rs = 0.85-0.93; p<0.0001). However, the ether-based concentration technique and the Parasep SF detected significantly fewer eggs than both the McMaster and the FLOTAC (p<0.0083). Overall, the McMaster was the most feasible technique (3.9 min/sample for preparing, reading and cleaning of the apparatus), followed by the ether-based concentration technique (7.7 min/sample) and the FLOTAC (9.8 min/sample). Parasep SF was the least feasible (17.7 min/sample). The simulation revealed that the sensitivity is less important for monitoring drug efficacy and that both FLOTAC and McMaster were reliable estimators. CONCLUSIONS/SIGNIFICANCE: The results of this study demonstrated that McMaster is a promising technique when making use of FEC to monitor drug efficacy in Trichuris. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/19172171/Field_validity_and_feasibility_of_four_techniques_for_the_detection_of_Trichuris_in_simians:_a_model_for_monitoring_drug_efficacy_in_public_health L2 - https://dx.plos.org/10.1371/journal.pntd.0000366 DB - PRIME DP - Unbound Medicine ER -