TY - JOUR T1 - Determination of anti-malaria agent chloroquine using single drop liquid-liquid-liquid microextraction. AU - Daneshfar,Ali, AU - Khezeli,Tahere, AU - Manafi,Mohamad H, PY - 2009/1/29/entrez PY - 2009/1/29/pubmed PY - 2009/4/14/medline SP - 511 EP - 6 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 4 N2 - A simple, sensitive, and inexpensive single drop liquid-liquid-liquid microextraction combined with isocratic RP-HPLC and UV detection was developed for the determination of anti-malaria drug, chloroquine. The target compound was extracted from alkaline aqueous sample solution (adjusted to 0.5 mol/L sodium hydroxide) through a thin layer of organic solvent membrane and back-extracted to an acidic acceptor drop (adjusted to 0.02 mol/L phosphoric acid) suspended on the tip of a 25 microL HPLC syringe in the organic layer. This syringe was also used for direct injection after extraction. The linear range was 1-200 microg/L. The LOD and LOQ were 0.3 and 1.0 microg/L, respectively. Intra-and inter-day precisions were less than 2.0 and 2.3%, respectively. The real samples were successfully analyzed using the proposed method. The recoveries of spiked samples were more than 94.6%. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19173322/Determination_of_anti_malaria_agent_chloroquine_using_single_drop_liquid_liquid_liquid_microextraction_ L2 - https://doi.org/10.1002/jssc.200800483 DB - PRIME DP - Unbound Medicine ER -