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Red blood cell delta15N: a novel biomarker of dietary eicosapentaenoic acid and docosahexaenoic acid intake.
Am J Clin Nutr. 2009 Mar; 89(3):913-9.AJ

Abstract

BACKGROUND

The long-chain omega-3 (n-3) fatty acids derived from fish, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are associated with a reduced risk of cardiovascular disease and other chronic diseases. Study of the associations between EPA and DHA intake and disease requires a valid biomarker of dietary intake; however, the direct measurement of tissue fatty acid concentrations is expensive and time consuming.

OBJECTIVE

Because the nitrogen stable isotope ratio ((15)N/(14)N, expressed as delta(15)N) is elevated in fish, we investigated whether delta(15)N is a valid alternative biomarker of EPA and DHA intake.

DESIGN

We examined the relation between red blood cell (RBC) delta(15)N and RBC EPA and DHA in a community-based sample of 496 Yup'ik Eskimos with widely varying intake of n-3 fatty acids. We also assessed the correlation between delta(15)N and dietary EPA and DHA intake based on 24-h dietary recalls and 3-d food records completed by a subset of 221 participants.

RESULTS

RBC delta(15)N was strongly correlated with RBC EPA and DHA (r = 0.83 and 0.75, respectively). These correlations differed only modestly by sex and age class. RBC delta(15)N also correlated with dietary EPA and DHA intake (r = 0.47 and 0.46, respectively) and did not differ by sex and age.

CONCLUSIONS

The results strongly support the validity of RBC delta(15)N as a biomarker of EPA and DHA intake. Because the analysis of RBC delta(15)N is rapid and inexpensive, this method could facilitate wide-scale assessment of EPA and DHA intake in clinical and epidemiologic studies.

Authors+Show Affiliations

Center for Alaska Native Health Research, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775-7000, USA. ffdo@uaf.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19176727

Citation

O'Brien, Diane M., et al. "Red Blood Cell delta15N: a Novel Biomarker of Dietary Eicosapentaenoic Acid and Docosahexaenoic Acid Intake." The American Journal of Clinical Nutrition, vol. 89, no. 3, 2009, pp. 913-9.
O'Brien DM, Kristal AR, Jeannet MA, et al. Red blood cell delta15N: a novel biomarker of dietary eicosapentaenoic acid and docosahexaenoic acid intake. Am J Clin Nutr. 2009;89(3):913-9.
O'Brien, D. M., Kristal, A. R., Jeannet, M. A., Wilkinson, M. J., Bersamin, A., & Luick, B. (2009). Red blood cell delta15N: a novel biomarker of dietary eicosapentaenoic acid and docosahexaenoic acid intake. The American Journal of Clinical Nutrition, 89(3), 913-9. https://doi.org/10.3945/ajcn.2008.27054
O'Brien DM, et al. Red Blood Cell delta15N: a Novel Biomarker of Dietary Eicosapentaenoic Acid and Docosahexaenoic Acid Intake. Am J Clin Nutr. 2009;89(3):913-9. PubMed PMID: 19176727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Red blood cell delta15N: a novel biomarker of dietary eicosapentaenoic acid and docosahexaenoic acid intake. AU - O'Brien,Diane M, AU - Kristal,Alan R, AU - Jeannet,M Alyssa, AU - Wilkinson,Michael J, AU - Bersamin,Andrea, AU - Luick,Bret, Y1 - 2009/01/28/ PY - 2009/1/30/entrez PY - 2009/1/30/pubmed PY - 2009/3/19/medline SP - 913 EP - 9 JF - The American journal of clinical nutrition JO - Am J Clin Nutr VL - 89 IS - 3 N2 - BACKGROUND: The long-chain omega-3 (n-3) fatty acids derived from fish, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are associated with a reduced risk of cardiovascular disease and other chronic diseases. Study of the associations between EPA and DHA intake and disease requires a valid biomarker of dietary intake; however, the direct measurement of tissue fatty acid concentrations is expensive and time consuming. OBJECTIVE: Because the nitrogen stable isotope ratio ((15)N/(14)N, expressed as delta(15)N) is elevated in fish, we investigated whether delta(15)N is a valid alternative biomarker of EPA and DHA intake. DESIGN: We examined the relation between red blood cell (RBC) delta(15)N and RBC EPA and DHA in a community-based sample of 496 Yup'ik Eskimos with widely varying intake of n-3 fatty acids. We also assessed the correlation between delta(15)N and dietary EPA and DHA intake based on 24-h dietary recalls and 3-d food records completed by a subset of 221 participants. RESULTS: RBC delta(15)N was strongly correlated with RBC EPA and DHA (r = 0.83 and 0.75, respectively). These correlations differed only modestly by sex and age class. RBC delta(15)N also correlated with dietary EPA and DHA intake (r = 0.47 and 0.46, respectively) and did not differ by sex and age. CONCLUSIONS: The results strongly support the validity of RBC delta(15)N as a biomarker of EPA and DHA intake. Because the analysis of RBC delta(15)N is rapid and inexpensive, this method could facilitate wide-scale assessment of EPA and DHA intake in clinical and epidemiologic studies. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/19176727/Red_blood_cell_delta15N:_a_novel_biomarker_of_dietary_eicosapentaenoic_acid_and_docosahexaenoic_acid_intake_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.3945/ajcn.2008.27054 DB - PRIME DP - Unbound Medicine ER -