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A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences.
J Clin Virol. 2009 Mar; 44(3):195-9.JC

Abstract

BACKGROUND

Although hepatitis C virus (HCV) genotype 6 is classified into 21 subtypes, 6a-6u, new variants continue to be identified.

OBJECTIVE

To characterize the full-length genomes of three novel HCV genotype 6 variants: KMN02, KM046 and KM181.

STUDY DESIGN

From sera of patients with HCV infection, the entire HCV genome was amplified by RT-PCR followed by direct DNA sequencing and phylogenetic analysis.

RESULTS

The sera contained HCV genomes of 9461, 9429, and 9461nt in length, and each harboured a single ORF of 9051nt. The genomes showed 95.3-98.1% nucleotide similarity to each other and 72.2-75.4% similarity to 23 genotype 6 reference sequences, which represent subtypes 6a-6u and unassigned variants km41 and gz52557. Phylogenetic analyses demonstrated that they were genotype 6, but were subtypically distinct. Based on the current criteria of HCV classification, they were designed to represent a new subtype, 6v. Analysis of E1 and NS5B region partial sequences revealed two additional related variants, CMBD-14 and CMBD-86 that had been previously reported in northern Thailand and sequences dropped into Genbank.

CONCLUSION

Three novel HCV genotype 6 variants were entirely sequenced and designated subtype 6v.

Authors+Show Affiliations

Sun Yat-sen University, Guangzhou, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19179105

Citation

Wang, Yizhong, et al. "A New HCV Genotype 6 Subtype Designated 6v Was Confirmed With Three Complete Genome Sequences." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 44, no. 3, 2009, pp. 195-9.
Wang Y, Xia X, Li C, et al. A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences. J Clin Virol. 2009;44(3):195-9.
Wang, Y., Xia, X., Li, C., Maneekarn, N., Xia, W., Zhao, W., Feng, Y., Kung, H. F., Fu, Y., & Lu, L. (2009). A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 44(3), 195-9. https://doi.org/10.1016/j.jcv.2008.12.009
Wang Y, et al. A New HCV Genotype 6 Subtype Designated 6v Was Confirmed With Three Complete Genome Sequences. J Clin Virol. 2009;44(3):195-9. PubMed PMID: 19179105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences. AU - Wang,Yizhong, AU - Xia,Xueshan, AU - Li,Chunhua, AU - Maneekarn,Niwat, AU - Xia,Wenjie, AU - Zhao,Wenhua, AU - Feng,Yue, AU - Kung,Hsiang Fu, AU - Fu,Yongshui, AU - Lu,Ling, PY - 2008/07/16/received PY - 2008/12/12/revised PY - 2008/12/17/accepted PY - 2009/1/31/entrez PY - 2009/1/31/pubmed PY - 2009/4/14/medline SP - 195 EP - 9 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 44 IS - 3 N2 - BACKGROUND: Although hepatitis C virus (HCV) genotype 6 is classified into 21 subtypes, 6a-6u, new variants continue to be identified. OBJECTIVE: To characterize the full-length genomes of three novel HCV genotype 6 variants: KMN02, KM046 and KM181. STUDY DESIGN: From sera of patients with HCV infection, the entire HCV genome was amplified by RT-PCR followed by direct DNA sequencing and phylogenetic analysis. RESULTS: The sera contained HCV genomes of 9461, 9429, and 9461nt in length, and each harboured a single ORF of 9051nt. The genomes showed 95.3-98.1% nucleotide similarity to each other and 72.2-75.4% similarity to 23 genotype 6 reference sequences, which represent subtypes 6a-6u and unassigned variants km41 and gz52557. Phylogenetic analyses demonstrated that they were genotype 6, but were subtypically distinct. Based on the current criteria of HCV classification, they were designed to represent a new subtype, 6v. Analysis of E1 and NS5B region partial sequences revealed two additional related variants, CMBD-14 and CMBD-86 that had been previously reported in northern Thailand and sequences dropped into Genbank. CONCLUSION: Three novel HCV genotype 6 variants were entirely sequenced and designated subtype 6v. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/19179105/A_new_HCV_genotype_6_subtype_designated_6v_was_confirmed_with_three_complete_genome_sequences_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(08)00470-8 DB - PRIME DP - Unbound Medicine ER -