TY - JOUR T1 - Analysis of aliphatic amines using head-column field-enhanced sample stacking in MEKC with LIF detection. AU - Zhang,Jianhua, AU - Cui,Huimin, AU - Xu,Liangjun, AU - Zhang,Lan, AU - Chen,Guonan, PY - 2009/1/31/entrez PY - 2009/1/31/pubmed PY - 2009/7/21/medline SP - 674 EP - 81 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 4 N2 - This paper describes the use of head-column field-enhanced sample stacking for on-line sample concentration in MEKC; the method has been applied to the determination of C1-C9 aliphatic amines after their pre-capillary derivatization with 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA). The optimum derivatization, on-line concentration and separation conditions for these aliphatic amines were investigated in detail. The optimized molar ratio of CBQCA to amines was found to be 120:1, and 50 mmol/L borate buffer (pH 9.5) was used for derivatization in order to achieve high efficiency. Nine imidazole aliphatic amines were baseline separated within 12 min by using 50 mmol/L of sodium borate (pH 9.5) and 15 mmol/L of SDS as running buffer. Under the optimum conditions, the detection limits of these nine aliphatic amines ranged from 1.2 to 3.8 pmol/L. S/N=3, which was about 49-173-fold lower than those of conventional sample injections. After validation, the developed method was applied to the determination of C1-C9 aliphatic amines in river water with the average recoveries of 86.7-105%. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19180544/Analysis_of_aliphatic_amines_using_head_column_field_enhanced_sample_stacking_in_MEKC_with_LIF_detection_ L2 - https://doi.org/10.1002/elps.200800569 DB - PRIME DP - Unbound Medicine ER -