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Analysis of aliphatic amines using head-column field-enhanced sample stacking in MEKC with LIF detection.
Electrophoresis. 2009 Feb; 30(4):674-81.E

Abstract

This paper describes the use of head-column field-enhanced sample stacking for on-line sample concentration in MEKC; the method has been applied to the determination of C1-C9 aliphatic amines after their pre-capillary derivatization with 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA). The optimum derivatization, on-line concentration and separation conditions for these aliphatic amines were investigated in detail. The optimized molar ratio of CBQCA to amines was found to be 120:1, and 50 mmol/L borate buffer (pH 9.5) was used for derivatization in order to achieve high efficiency. Nine imidazole aliphatic amines were baseline separated within 12 min by using 50 mmol/L of sodium borate (pH 9.5) and 15 mmol/L of SDS as running buffer. Under the optimum conditions, the detection limits of these nine aliphatic amines ranged from 1.2 to 3.8 pmol/L. S/N=3, which was about 49-173-fold lower than those of conventional sample injections. After validation, the developed method was applied to the determination of C1-C9 aliphatic amines in river water with the average recoveries of 86.7-105%.

Authors+Show Affiliations

Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19180544

Citation

Zhang, Jianhua, et al. "Analysis of Aliphatic Amines Using Head-column Field-enhanced Sample Stacking in MEKC With LIF Detection." Electrophoresis, vol. 30, no. 4, 2009, pp. 674-81.
Zhang J, Cui H, Xu L, et al. Analysis of aliphatic amines using head-column field-enhanced sample stacking in MEKC with LIF detection. Electrophoresis. 2009;30(4):674-81.
Zhang, J., Cui, H., Xu, L., Zhang, L., & Chen, G. (2009). Analysis of aliphatic amines using head-column field-enhanced sample stacking in MEKC with LIF detection. Electrophoresis, 30(4), 674-81. https://doi.org/10.1002/elps.200800569
Zhang J, et al. Analysis of Aliphatic Amines Using Head-column Field-enhanced Sample Stacking in MEKC With LIF Detection. Electrophoresis. 2009;30(4):674-81. PubMed PMID: 19180544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of aliphatic amines using head-column field-enhanced sample stacking in MEKC with LIF detection. AU - Zhang,Jianhua, AU - Cui,Huimin, AU - Xu,Liangjun, AU - Zhang,Lan, AU - Chen,Guonan, PY - 2009/1/31/entrez PY - 2009/1/31/pubmed PY - 2009/7/21/medline SP - 674 EP - 81 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 4 N2 - This paper describes the use of head-column field-enhanced sample stacking for on-line sample concentration in MEKC; the method has been applied to the determination of C1-C9 aliphatic amines after their pre-capillary derivatization with 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA). The optimum derivatization, on-line concentration and separation conditions for these aliphatic amines were investigated in detail. The optimized molar ratio of CBQCA to amines was found to be 120:1, and 50 mmol/L borate buffer (pH 9.5) was used for derivatization in order to achieve high efficiency. Nine imidazole aliphatic amines were baseline separated within 12 min by using 50 mmol/L of sodium borate (pH 9.5) and 15 mmol/L of SDS as running buffer. Under the optimum conditions, the detection limits of these nine aliphatic amines ranged from 1.2 to 3.8 pmol/L. S/N=3, which was about 49-173-fold lower than those of conventional sample injections. After validation, the developed method was applied to the determination of C1-C9 aliphatic amines in river water with the average recoveries of 86.7-105%. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19180544/Analysis_of_aliphatic_amines_using_head_column_field_enhanced_sample_stacking_in_MEKC_with_LIF_detection_ L2 - https://doi.org/10.1002/elps.200800569 DB - PRIME DP - Unbound Medicine ER -