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[Relationship between the effect of vascular endothelial growth factor on epithelial-mesenchymal transition of HK-2 cells and the expressions of bone morphogenetic protein-7 and inhibitor of DNA binding/differentiation].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008 Dec; 30(6):703-10.ZY

Abstract

OBJECTIVE

To examine the relationship between effect of vascular endothelial growth factor (VEGF) on epithelial-myofibroblast transition (EMT) of HK-2 cells and changes in expressions of bone morphogenetic protein-7 (BMP-7) and inhibitor of DNA binding/differentiation (Id) 2, Id3.

METHODS

The cultured HK-2 cells were co-treated with transforming growth factor-beta1 (TGF-beta1) (5 ng/ml) and VEGF165 (0.1, 1, 10, 100 ng/ml), or with TGF-beta1 (5 ng/ml) and VEGF receptor-1 neutralized antibody (10 microg/ ml), and were also co-treated with TGF-beta1 (5 ng/ml) and VEGF165 (100 ng/ml) with or without activin receptor-like kinase 6 (Alk6)/Fc Chimera (2 microg/ml, to neutralize endogenous BMP-7) for 48 hours. mRNA and protein expressions of alpha-smooth muscle actin (alpha-SMA), E-cadherin, BMP-7, Id2 and Id3 of HK-2 cells were assessed with double-stain immunocytochemistry, real-time PCR and Western blot respectively.

RESULTS

Compared with normal controls, alpha-SMA expression significantly increased, while E-cadherin, BMP-7, Id2, and Id3 mRNA and protein expressions markedly decreased in HK-2 cells treated with TGF-beta1 (5 ng/ml) (P < 0.05). VEGF165 interrupted TGF-beta1 induced alpha-SMA expression in a dose-dependent manner and upregulated BMP-7, Id2 mRNA and protein expressions of the cells (P < 0.05). alpha-SMA expression increased, while E-cadherin, BMP-7, and Id2 expressions decreased further in HK-2 cells co-treated with TGF-beta1 and VEGFR1 antibody compared with normal controls (P < 0.05). When endogenous BMP-7 was neutralized with Alk6/Fc Chimera in the cells co-treated with TGF-beta1 and VEGF165, alpha-SMA expression upregulated (P < 0.05), while Id2 was not changed.

CONCLUSIONS

VEGF165 may partially inhibit TGF-beta1-induced EMT of HK-2 cells in vitro. This effect is related to the upregulated expressions of BMP-7 and Id2. Id2 may be upregulated directly by VEGF165, but not related to BMP-7.

Authors+Show Affiliations

He CM
Department of Nephrology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zheng FL
No affiliation info available
Lian YG
No affiliation info available
Liu YP
No affiliation info available

MeSH

Bone Morphogenetic Protein 7Cell DifferentiationCell LineEpithelial CellsGene Expression RegulationHumansInhibitor of Differentiation Protein 2Inhibitor of Differentiation ProteinsNeoplasm ProteinsTransforming Growth Factor beta1Vascular Endothelial Growth Factor A

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19180920

Citation

He, Chun-Mei, et al. "[Relationship Between the Effect of Vascular Endothelial Growth Factor On Epithelial-mesenchymal Transition of HK-2 Cells and the Expressions of Bone Morphogenetic Protein-7 and Inhibitor of DNA Binding/differentiation]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 30, no. 6, 2008, pp. 703-10.
He CM, Zheng FL, Lian YG, et al. [Relationship between the effect of vascular endothelial growth factor on epithelial-mesenchymal transition of HK-2 cells and the expressions of bone morphogenetic protein-7 and inhibitor of DNA binding/differentiation]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008;30(6):703-10.
He, C. M., Zheng, F. L., Lian, Y. G., & Liu, Y. P. (2008). [Relationship between the effect of vascular endothelial growth factor on epithelial-mesenchymal transition of HK-2 cells and the expressions of bone morphogenetic protein-7 and inhibitor of DNA binding/differentiation]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 30(6), 703-10.
He CM, et al. [Relationship Between the Effect of Vascular Endothelial Growth Factor On Epithelial-mesenchymal Transition of HK-2 Cells and the Expressions of Bone Morphogenetic Protein-7 and Inhibitor of DNA Binding/differentiation]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008;30(6):703-10. PubMed PMID: 19180920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Relationship between the effect of vascular endothelial growth factor on epithelial-mesenchymal transition of HK-2 cells and the expressions of bone morphogenetic protein-7 and inhibitor of DNA binding/differentiation]. AU - He,Chun-Mei, AU - Zheng,Fa-Lei, AU - Lian,Yao-Guo, AU - Liu,Yan-Ping, PY - 2009/2/3/entrez PY - 2009/2/3/pubmed PY - 2010/6/4/medline SP - 703 EP - 10 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 30 IS - 6 N2 - OBJECTIVE: To examine the relationship between effect of vascular endothelial growth factor (VEGF) on epithelial-myofibroblast transition (EMT) of HK-2 cells and changes in expressions of bone morphogenetic protein-7 (BMP-7) and inhibitor of DNA binding/differentiation (Id) 2, Id3. METHODS: The cultured HK-2 cells were co-treated with transforming growth factor-beta1 (TGF-beta1) (5 ng/ml) and VEGF165 (0.1, 1, 10, 100 ng/ml), or with TGF-beta1 (5 ng/ml) and VEGF receptor-1 neutralized antibody (10 microg/ ml), and were also co-treated with TGF-beta1 (5 ng/ml) and VEGF165 (100 ng/ml) with or without activin receptor-like kinase 6 (Alk6)/Fc Chimera (2 microg/ml, to neutralize endogenous BMP-7) for 48 hours. mRNA and protein expressions of alpha-smooth muscle actin (alpha-SMA), E-cadherin, BMP-7, Id2 and Id3 of HK-2 cells were assessed with double-stain immunocytochemistry, real-time PCR and Western blot respectively. RESULTS: Compared with normal controls, alpha-SMA expression significantly increased, while E-cadherin, BMP-7, Id2, and Id3 mRNA and protein expressions markedly decreased in HK-2 cells treated with TGF-beta1 (5 ng/ml) (P < 0.05). VEGF165 interrupted TGF-beta1 induced alpha-SMA expression in a dose-dependent manner and upregulated BMP-7, Id2 mRNA and protein expressions of the cells (P < 0.05). alpha-SMA expression increased, while E-cadherin, BMP-7, and Id2 expressions decreased further in HK-2 cells co-treated with TGF-beta1 and VEGFR1 antibody compared with normal controls (P < 0.05). When endogenous BMP-7 was neutralized with Alk6/Fc Chimera in the cells co-treated with TGF-beta1 and VEGF165, alpha-SMA expression upregulated (P < 0.05), while Id2 was not changed. CONCLUSIONS: VEGF165 may partially inhibit TGF-beta1-induced EMT of HK-2 cells in vitro. This effect is related to the upregulated expressions of BMP-7 and Id2. Id2 may be upregulated directly by VEGF165, but not related to BMP-7. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/19180920/[Relationship_between_the_effect_of_vascular_endothelial_growth_factor_on_epithelial_mesenchymal_transition_of_HK_2_cells_and_the_expressions_of_bone_morphogenetic_protein_7_and_inhibitor_of_DNA_binding/differentiation]_ L2 - https://antibodies.cancer.gov/detail/CPTC-CDH1-3 DB - PRIME DP - Unbound Medicine ER -

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