Tags

Type your tag names separated by a space and hit enter

Differential evaluation of excisional non-occluded wound healing in db/db mice.
Toxicol Pathol 2009; 37(2):183-92TP

Abstract

The full-thickness wound in the genetically diabetic (db/db) mouse is a commonly used model of impaired wound healing. We investigated delayed healing of non-occluded, excisional, full-thickness, dermal wounds in db/db mice in comparison to their normal littermate controls and refined methods for monitoring skin wound re-epithelialization, contraction, granulation tissue formation, and inflammation. We have confirmed with a computer-assisted planimetry method the results of previous studies showing that healing of non-occluded full excision wounds in db/db mice does not occur by contraction as much as in healthy mice. In addition, we have developed separate histological methods for the assessment of re-epithelialization, contraction, granulation tissue (mature, immature, fibrosis), and inflammation (lipogranulomas, secondary, nonspecific). Using a new approach to histological assessment, we have shown that wound closure in db/db mice is delayed owing to: (1) delayed granulation tissue maturation; (2) ''laced,'' widely distributed granulation tissue around fat lobules; and (3) obstruction by lipogranulomas, whereas the rate of re-epithelialization seems to be the same as in C57Bl/6 mice. This methodology should permit a more precise differentiation of effects of novel therapeutic agents on the wound healing process in db/db mice.

Authors+Show Affiliations

GSK Research Centre Zagreb Ltd., Prilaz baruna Filipovića 29, Zagreb, Croatia. vanesa.i.ivetic-tkalcevic@gsk.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Studies
Journal Article

Language

eng

PubMed ID

19182213

Citation

Tkalcević, Vanesa Ivetić, et al. "Differential Evaluation of Excisional Non-occluded Wound Healing in Db/db Mice." Toxicologic Pathology, vol. 37, no. 2, 2009, pp. 183-92.
Tkalcević VI, Cuzić S, Parnham MJ, et al. Differential evaluation of excisional non-occluded wound healing in db/db mice. Toxicol Pathol. 2009;37(2):183-92.
Tkalcević, V. I., Cuzić, S., Parnham, M. J., Pasalić, I., & Brajsa, K. (2009). Differential evaluation of excisional non-occluded wound healing in db/db mice. Toxicologic Pathology, 37(2), pp. 183-92. doi:10.1177/0192623308329280.
Tkalcević VI, et al. Differential Evaluation of Excisional Non-occluded Wound Healing in Db/db Mice. Toxicol Pathol. 2009;37(2):183-92. PubMed PMID: 19182213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential evaluation of excisional non-occluded wound healing in db/db mice. AU - Tkalcević,Vanesa Ivetić, AU - Cuzić,Snjezana, AU - Parnham,Michael J, AU - Pasalić,Ivanka, AU - Brajsa,Karmen, Y1 - 2009/01/30/ PY - 2009/2/3/entrez PY - 2009/2/3/pubmed PY - 2009/6/17/medline SP - 183 EP - 92 JF - Toxicologic pathology JO - Toxicol Pathol VL - 37 IS - 2 N2 - The full-thickness wound in the genetically diabetic (db/db) mouse is a commonly used model of impaired wound healing. We investigated delayed healing of non-occluded, excisional, full-thickness, dermal wounds in db/db mice in comparison to their normal littermate controls and refined methods for monitoring skin wound re-epithelialization, contraction, granulation tissue formation, and inflammation. We have confirmed with a computer-assisted planimetry method the results of previous studies showing that healing of non-occluded full excision wounds in db/db mice does not occur by contraction as much as in healthy mice. In addition, we have developed separate histological methods for the assessment of re-epithelialization, contraction, granulation tissue (mature, immature, fibrosis), and inflammation (lipogranulomas, secondary, nonspecific). Using a new approach to histological assessment, we have shown that wound closure in db/db mice is delayed owing to: (1) delayed granulation tissue maturation; (2) ''laced,'' widely distributed granulation tissue around fat lobules; and (3) obstruction by lipogranulomas, whereas the rate of re-epithelialization seems to be the same as in C57Bl/6 mice. This methodology should permit a more precise differentiation of effects of novel therapeutic agents on the wound healing process in db/db mice. SN - 1533-1601 UR - https://www.unboundmedicine.com/medline/citation/19182213/Differential_evaluation_of_excisional_non_occluded_wound_healing_in_db/db_mice_ L2 - http://journals.sagepub.com/doi/full/10.1177/0192623308329280?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -