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Preparation and antioxidant activity of green tea extract enriched in epigallocatechin (EGC) and epigallocatechin gallate (EGCG).
J Agric Food Chem. 2009 Feb 25; 57(4):1349-53.JA

Abstract

The present study investigated effects of solvents, temperature, and duration on extracting efficiency of catechins from green tea by an orthogonal test. The results suggested that extraction of epigallocatechin gallate (EGCG) was highly dependent on these factors, whereas that of epigallocatechin (EGC) was significantly affected by duration and solvent (P < 0.05). Effects of these factors on epicatechin (EC), catechin (C), and epicatechin gallate (ECG) were not significant. A two-step preparation was adopted to produce green tea extract enriched in EGC (GTE-EGC) and green tea extract enriched in EGCG (GTE-EGCG). The optimum conditions for GTE-EGC were that green tea was brewed in 75% ethanol at 30 degrees C for 10 min, whereas for GTE-EGCG the same tea was brewed in 35% ethanol at 90 degrees C for 60 min. Compared with GTE-EGC, GTE-EGCG had EGCG increased by 110.42%, whereas EGC decreased by 40.38% with EC and ECG being unchanged. Most importantly, GTE-EGCG possessed greater antioxidant activity (in vitro) than GTE-EGC.

Authors+Show Affiliations

College of Horticulture and Forestry Science, Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology, Ministry of Education, No. 1 Shizishan Street, Hongshan District, Wuhan City, Hubei Province, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19182914

Citation

Hu, Jianhui, et al. "Preparation and Antioxidant Activity of Green Tea Extract Enriched in Epigallocatechin (EGC) and Epigallocatechin Gallate (EGCG)." Journal of Agricultural and Food Chemistry, vol. 57, no. 4, 2009, pp. 1349-53.
Hu J, Zhou D, Chen Y. Preparation and antioxidant activity of green tea extract enriched in epigallocatechin (EGC) and epigallocatechin gallate (EGCG). J Agric Food Chem. 2009;57(4):1349-53.
Hu, J., Zhou, D., & Chen, Y. (2009). Preparation and antioxidant activity of green tea extract enriched in epigallocatechin (EGC) and epigallocatechin gallate (EGCG). Journal of Agricultural and Food Chemistry, 57(4), 1349-53. https://doi.org/10.1021/jf803143n
Hu J, Zhou D, Chen Y. Preparation and Antioxidant Activity of Green Tea Extract Enriched in Epigallocatechin (EGC) and Epigallocatechin Gallate (EGCG). J Agric Food Chem. 2009 Feb 25;57(4):1349-53. PubMed PMID: 19182914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and antioxidant activity of green tea extract enriched in epigallocatechin (EGC) and epigallocatechin gallate (EGCG). AU - Hu,Jianhui, AU - Zhou,Danrong, AU - Chen,Yuqiong, PY - 2009/2/3/entrez PY - 2009/2/3/pubmed PY - 2009/4/22/medline SP - 1349 EP - 53 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 4 N2 - The present study investigated effects of solvents, temperature, and duration on extracting efficiency of catechins from green tea by an orthogonal test. The results suggested that extraction of epigallocatechin gallate (EGCG) was highly dependent on these factors, whereas that of epigallocatechin (EGC) was significantly affected by duration and solvent (P < 0.05). Effects of these factors on epicatechin (EC), catechin (C), and epicatechin gallate (ECG) were not significant. A two-step preparation was adopted to produce green tea extract enriched in EGC (GTE-EGC) and green tea extract enriched in EGCG (GTE-EGCG). The optimum conditions for GTE-EGC were that green tea was brewed in 75% ethanol at 30 degrees C for 10 min, whereas for GTE-EGCG the same tea was brewed in 35% ethanol at 90 degrees C for 60 min. Compared with GTE-EGC, GTE-EGCG had EGCG increased by 110.42%, whereas EGC decreased by 40.38% with EC and ECG being unchanged. Most importantly, GTE-EGCG possessed greater antioxidant activity (in vitro) than GTE-EGC. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19182914/Preparation_and_antioxidant_activity_of_green_tea_extract_enriched_in_epigallocatechin__EGC__and_epigallocatechin_gallate__EGCG__ L2 - https://doi.org/10.1021/jf803143n DB - PRIME DP - Unbound Medicine ER -