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Lonicera hypoglauca inhibits xanthine oxidase and reduces serum uric acid in mice.
Planta Med. 2009 Mar; 75(4):302-6.PM

Abstract

Xanthine oxidase (XOD) catalyzes the oxidation of hypoxanthine to xanthine and then to uric acid, and is a key enzyme in the pathogenesis of hyperuricemia. The ability of extracts of Lonicera hypoglauca (Caprifoliaceae) to inhibit XOD was investigated in this study. An ethanol extract (LH-crude) of the leaves of L. hypoglauca and its derived EtOAc soluble sub-fractions (LH-EA) significantly inhibited XOD activity, with IC50 values for LH-crude and LH-EA of 48.8 and 35.2 microg/mL. Moreover, LH-EA reduced serum urate levels IN VIVO in a potassium oxonate-induced hyperuricemic mouse model, by 70.1% and 93.7% of the hyperuricemic untreated group at doses of 300 and 500 mg/kg of LH-EA, respectively. Finally, we used bioactivity-guided fractionation to isolate a new bisflavonoid, loniceraflavone, which showed significant inhibition of XOD (IC50=0.85 microg/mL). These results suggest that L. hypoglauca and its extracts may have a considerable potential for development as an anti-hyperuricemia agent for clinical application.

Authors+Show Affiliations

School of Chinese Medicine Resources, China Medical University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19184967

Citation

Chien, Shih-Chang, et al. "Lonicera Hypoglauca Inhibits Xanthine Oxidase and Reduces Serum Uric Acid in Mice." Planta Medica, vol. 75, no. 4, 2009, pp. 302-6.
Chien SC, Yang CW, Tseng YH, et al. Lonicera hypoglauca inhibits xanthine oxidase and reduces serum uric acid in mice. Planta Med. 2009;75(4):302-6.
Chien, S. C., Yang, C. W., Tseng, Y. H., Tsay, H. S., Kuo, Y. H., & Wang, S. Y. (2009). Lonicera hypoglauca inhibits xanthine oxidase and reduces serum uric acid in mice. Planta Medica, 75(4), 302-6. https://doi.org/10.1055/s-0029-1185300
Chien SC, et al. Lonicera Hypoglauca Inhibits Xanthine Oxidase and Reduces Serum Uric Acid in Mice. Planta Med. 2009;75(4):302-6. PubMed PMID: 19184967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lonicera hypoglauca inhibits xanthine oxidase and reduces serum uric acid in mice. AU - Chien,Shih-Chang, AU - Yang,Chen-Wei, AU - Tseng,Yen-Hsueh, AU - Tsay,Hsin-Sheng, AU - Kuo,Yueh-Hsiung, AU - Wang,Sheng-Yang, Y1 - 2009/01/30/ PY - 2009/2/3/entrez PY - 2009/2/3/pubmed PY - 2009/5/21/medline SP - 302 EP - 6 JF - Planta medica JO - Planta Med. VL - 75 IS - 4 N2 - Xanthine oxidase (XOD) catalyzes the oxidation of hypoxanthine to xanthine and then to uric acid, and is a key enzyme in the pathogenesis of hyperuricemia. The ability of extracts of Lonicera hypoglauca (Caprifoliaceae) to inhibit XOD was investigated in this study. An ethanol extract (LH-crude) of the leaves of L. hypoglauca and its derived EtOAc soluble sub-fractions (LH-EA) significantly inhibited XOD activity, with IC50 values for LH-crude and LH-EA of 48.8 and 35.2 microg/mL. Moreover, LH-EA reduced serum urate levels IN VIVO in a potassium oxonate-induced hyperuricemic mouse model, by 70.1% and 93.7% of the hyperuricemic untreated group at doses of 300 and 500 mg/kg of LH-EA, respectively. Finally, we used bioactivity-guided fractionation to isolate a new bisflavonoid, loniceraflavone, which showed significant inhibition of XOD (IC50=0.85 microg/mL). These results suggest that L. hypoglauca and its extracts may have a considerable potential for development as an anti-hyperuricemia agent for clinical application. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/19184967/Lonicera_hypoglauca_inhibits_xanthine_oxidase_and_reduces_serum_uric_acid_in_mice_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1185300 DB - PRIME DP - Unbound Medicine ER -