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Automated polyvinylidene difluoride hollow fiber liquid-phase microextraction of flunitrazepam in plasma and urine samples for gas chromatography/tandem mass spectrometry.
J Chromatogr A. 2009 Mar 20; 1216(12):2241-7.JC

Abstract

A new polyvinylidene difluoride (PVDF) hollow fiber (200 microm wall thickness, 1.2mm internal diameter, 0.2 microm pore size) was compared with two other polypropylene (PP) hollow fibers (200, 300 microm wall thickness, 1.2mm internal diameter, 0.2 microm pore size) in the automated hollow fiber liquid-phase microextraction (HF-LPME) of flunitrazepam (FLNZ) in biological samples. With higher porosity and better solvent compatibility, the PVDF hollow fiber showed advantages with faster extraction efficiency and operational accuracy. Parameters of the CTC autosampler program for HF-LPME in plasma and urine samples were carefully investigated to ensure accuracy and reproducibility. Several parameters influencing the efficiency of HF-LPME of FLNZ in plasma and urine samples were optimized, including type of porous hollow fiber, organic solvent, agitation rate, extraction time, salt concentration, organic modifier, and pH. Under optimal conditions, extraction recoveries of FLNZ in plasma and urine samples were 6.5% and 83.5%, respectively, corresponding to the enrichment factor of 13 in plasma matrix and 167 in urine matrix. Excellent sample clean-up was observed and good linearities (r(2)=0.9979 for plasma sample and 0.9995 for urine sample) were obtained in the range of 0.1-1000 ng/mL (plasma sample) and 0.01-1000 ng/mL (urine sample). The limits of detection (S/N=3) were 0.025 ng/mL in plasma matrix and 0.001 ng/mL in urine matrix by gas chromatography/mass spectrometry/mass spectrometry.

Authors+Show Affiliations

Department of Biological Applied Engineering, Shenzhen Polytechnic, Shenzhen, China. shufencui@163.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19185867

Citation

Cui, Shufen, et al. "Automated Polyvinylidene Difluoride Hollow Fiber Liquid-phase Microextraction of Flunitrazepam in Plasma and Urine Samples for Gas Chromatography/tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1216, no. 12, 2009, pp. 2241-7.
Cui S, Tan S, Ouyang G, et al. Automated polyvinylidene difluoride hollow fiber liquid-phase microextraction of flunitrazepam in plasma and urine samples for gas chromatography/tandem mass spectrometry. J Chromatogr A. 2009;1216(12):2241-7.
Cui, S., Tan, S., Ouyang, G., & Pawliszyn, J. (2009). Automated polyvinylidene difluoride hollow fiber liquid-phase microextraction of flunitrazepam in plasma and urine samples for gas chromatography/tandem mass spectrometry. Journal of Chromatography. A, 1216(12), 2241-7. https://doi.org/10.1016/j.chroma.2009.01.022
Cui S, et al. Automated Polyvinylidene Difluoride Hollow Fiber Liquid-phase Microextraction of Flunitrazepam in Plasma and Urine Samples for Gas Chromatography/tandem Mass Spectrometry. J Chromatogr A. 2009 Mar 20;1216(12):2241-7. PubMed PMID: 19185867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Automated polyvinylidene difluoride hollow fiber liquid-phase microextraction of flunitrazepam in plasma and urine samples for gas chromatography/tandem mass spectrometry. AU - Cui,Shufen, AU - Tan,Shuo, AU - Ouyang,Gangfeng, AU - Pawliszyn,Janusz, Y1 - 2009/01/15/ PY - 2008/10/22/received PY - 2009/01/08/revised PY - 2009/01/12/accepted PY - 2009/2/3/entrez PY - 2009/2/3/pubmed PY - 2009/8/14/medline SP - 2241 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 12 N2 - A new polyvinylidene difluoride (PVDF) hollow fiber (200 microm wall thickness, 1.2mm internal diameter, 0.2 microm pore size) was compared with two other polypropylene (PP) hollow fibers (200, 300 microm wall thickness, 1.2mm internal diameter, 0.2 microm pore size) in the automated hollow fiber liquid-phase microextraction (HF-LPME) of flunitrazepam (FLNZ) in biological samples. With higher porosity and better solvent compatibility, the PVDF hollow fiber showed advantages with faster extraction efficiency and operational accuracy. Parameters of the CTC autosampler program for HF-LPME in plasma and urine samples were carefully investigated to ensure accuracy and reproducibility. Several parameters influencing the efficiency of HF-LPME of FLNZ in plasma and urine samples were optimized, including type of porous hollow fiber, organic solvent, agitation rate, extraction time, salt concentration, organic modifier, and pH. Under optimal conditions, extraction recoveries of FLNZ in plasma and urine samples were 6.5% and 83.5%, respectively, corresponding to the enrichment factor of 13 in plasma matrix and 167 in urine matrix. Excellent sample clean-up was observed and good linearities (r(2)=0.9979 for plasma sample and 0.9995 for urine sample) were obtained in the range of 0.1-1000 ng/mL (plasma sample) and 0.01-1000 ng/mL (urine sample). The limits of detection (S/N=3) were 0.025 ng/mL in plasma matrix and 0.001 ng/mL in urine matrix by gas chromatography/mass spectrometry/mass spectrometry. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19185867/Automated_polyvinylidene_difluoride_hollow_fiber_liquid_phase_microextraction_of_flunitrazepam_in_plasma_and_urine_samples_for_gas_chromatography/tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00064-8 DB - PRIME DP - Unbound Medicine ER -