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Adipose tissue, liver and pancreas structural alterations in C57BL/6 mice fed high-fat-high-sucrose diet supplemented with fish oil (n-3 fatty acid rich oil).
Exp Toxicol Pathol. 2010 Jan; 62(1):17-25.ET

Abstract

Fish oil treatment was used in reversing the morphological and metabolic changes of C57BL/6 mice fed high-fat-high-sucrose (HFHS) diet. Two-month-old male C57BL/6 mice were fed HFHS chow or standard chow (SC). At 3 months of age, HFHS mice were separated into an untreated group (HFHS) and a group treated with fish oil (HFHS-Fo, 1.5g/kg/day). At 4 months of age, HFHS fed mice had an increase in body mass (BM) and total body fat, when the animals were sacrificed. Both parameters were lower in HFHS-Fo than in HFHS mice. Plasma glucose and insulin levels were not affected among the groups, but HFHS and HFHS-Fo animals had higher homeostasis model assessment for insulin resistance HOMA-IR ratio. HFHS and HFHS-FO mice had increased plasma total cholesterol and LDL-C, HFHS-Fo increased plasma HDL-C and decreased triglycerides levels. The liver mass (LM) and the adipocytes' size were larger in HFHS mice, while HFHS-Fo mice had a lower LM and smaller adipocytes. The liver steatosis and hepatocyte binucleation were increased in HFHS mice, while HFHS-Fo mice had reduced liver steatosis and hepatocyte binucleation. HFHS-Fo mice had a lower pancreas mass, while HFHS animals had higher islet pancreatic diameter. The SC group showed strong expression for insulin, glucagon and a glucose transporter type 2 GLUT-2 in all pancreatic islets, while in HFHS mice there was less expression for GLUT-2. However, HFHS-Fo mice showed an increase of GLUT-2 expression. In conclusion, dietary fish oil treatment reduces body mass and fat pad adiposity, and also by reducing plasma TG and pancreatic islet hypertrophy in mice fed high-fat-high-sucrose diet. Furthermore, fish oil improves glucagon and GLUT-2 expressions when it is decreased in insulin, but in hepatocyte binucleation and hepatic steatosis where the effect is reduced.

Authors+Show Affiliations

Laboratório de Morfometria e Morfologia Cardiovascular, Centro Biomédico, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro 87 (fds) 20551-030 Rio de Janeiro, RJ, Brasil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19186042

Citation

Nascimento, Fernanda A M., et al. "Adipose Tissue, Liver and Pancreas Structural Alterations in C57BL/6 Mice Fed High-fat-high-sucrose Diet Supplemented With Fish Oil (n-3 Fatty Acid Rich Oil)." Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, vol. 62, no. 1, 2010, pp. 17-25.
Nascimento FA, Barbosa-da-Silva S, Fernandes-Santos C, et al. Adipose tissue, liver and pancreas structural alterations in C57BL/6 mice fed high-fat-high-sucrose diet supplemented with fish oil (n-3 fatty acid rich oil). Exp Toxicol Pathol. 2010;62(1):17-25.
Nascimento, F. A., Barbosa-da-Silva, S., Fernandes-Santos, C., Mandarim-de-Lacerda, C. A., & Aguila, M. B. (2010). Adipose tissue, liver and pancreas structural alterations in C57BL/6 mice fed high-fat-high-sucrose diet supplemented with fish oil (n-3 fatty acid rich oil). Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, 62(1), 17-25. https://doi.org/10.1016/j.etp.2008.12.008
Nascimento FA, et al. Adipose Tissue, Liver and Pancreas Structural Alterations in C57BL/6 Mice Fed High-fat-high-sucrose Diet Supplemented With Fish Oil (n-3 Fatty Acid Rich Oil). Exp Toxicol Pathol. 2010;62(1):17-25. PubMed PMID: 19186042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adipose tissue, liver and pancreas structural alterations in C57BL/6 mice fed high-fat-high-sucrose diet supplemented with fish oil (n-3 fatty acid rich oil). AU - Nascimento,Fernanda A M, AU - Barbosa-da-Silva,Sandra, AU - Fernandes-Santos,Caroline, AU - Mandarim-de-Lacerda,Carlos A, AU - Aguila,Marcia B, Y1 - 2009/01/30/ PY - 2008/08/23/received PY - 2008/11/27/revised PY - 2008/12/23/accepted PY - 2009/2/3/entrez PY - 2009/2/3/pubmed PY - 2010/4/20/medline SP - 17 EP - 25 JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie JO - Exp. Toxicol. Pathol. VL - 62 IS - 1 N2 - Fish oil treatment was used in reversing the morphological and metabolic changes of C57BL/6 mice fed high-fat-high-sucrose (HFHS) diet. Two-month-old male C57BL/6 mice were fed HFHS chow or standard chow (SC). At 3 months of age, HFHS mice were separated into an untreated group (HFHS) and a group treated with fish oil (HFHS-Fo, 1.5g/kg/day). At 4 months of age, HFHS fed mice had an increase in body mass (BM) and total body fat, when the animals were sacrificed. Both parameters were lower in HFHS-Fo than in HFHS mice. Plasma glucose and insulin levels were not affected among the groups, but HFHS and HFHS-Fo animals had higher homeostasis model assessment for insulin resistance HOMA-IR ratio. HFHS and HFHS-FO mice had increased plasma total cholesterol and LDL-C, HFHS-Fo increased plasma HDL-C and decreased triglycerides levels. The liver mass (LM) and the adipocytes' size were larger in HFHS mice, while HFHS-Fo mice had a lower LM and smaller adipocytes. The liver steatosis and hepatocyte binucleation were increased in HFHS mice, while HFHS-Fo mice had reduced liver steatosis and hepatocyte binucleation. HFHS-Fo mice had a lower pancreas mass, while HFHS animals had higher islet pancreatic diameter. The SC group showed strong expression for insulin, glucagon and a glucose transporter type 2 GLUT-2 in all pancreatic islets, while in HFHS mice there was less expression for GLUT-2. However, HFHS-Fo mice showed an increase of GLUT-2 expression. In conclusion, dietary fish oil treatment reduces body mass and fat pad adiposity, and also by reducing plasma TG and pancreatic islet hypertrophy in mice fed high-fat-high-sucrose diet. Furthermore, fish oil improves glucagon and GLUT-2 expressions when it is decreased in insulin, but in hepatocyte binucleation and hepatic steatosis where the effect is reduced. SN - 1618-1433 UR - https://www.unboundmedicine.com/medline/citation/19186042/Adipose_tissue_liver_and_pancreas_structural_alterations_in_C57BL/6_mice_fed_high_fat_high_sucrose_diet_supplemented_with_fish_oil__n_3_fatty_acid_rich_oil__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0940-2993(08)00185-1 DB - PRIME DP - Unbound Medicine ER -