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Molecular evidence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from São Paulo, Brazil.
J Clin Virol. 2009 Mar; 44(3):185-9.JC

Abstract

BACKGROUND

Using enzyme immunoassays and Western blot (Wb) tests, HTLV serodiagnosis yields indeterminate results in a significant number of cases.

OBJECTIVE

To determine the prevalence of HTLV infection among HTLV-seroindeterminate individuals.

STUDY DESIGN

We studied peripheral blood mononuclear cells from 65 anti-HTLV Wb-seroindeterminate individuals by attempting to amplify proviral DNA sequences (tax and pol) to identify HTLV-1 and HTLV-2 infections.

RESULTS

These 65 specimens exhibited predominantly (43%) anti-HTLV antibodies to gag-coded antigens in the absence of anti-p24 on Wb analysis. Tax proviral sequences were detected in 6 (9.2%) samples. According to restricted fragment polymorphism analysis (RFLP), we identified HTLV-1 proviral DNA in 4 samples, HTLV-2 in one and sequences from both in another. Nested PCR for the pol region was positive in 3 (4.6%) specimens, which were also positive for tax sequences. After hybridization HTLV-1 infection was confirmed in 2 samples (3.1%) and HTLV-2 in another (1.5%). Detection of a single HTLV DNA sequence may be due to infection by defective provirus, but its significance remains undefined. In this cohort, no Wb reactivity pattern was predictive of proviral detection. HTLV-1 infection was demonstrated in an individual who had Wb reactivity to gag-coded antigens only.

CONCLUSIONS

This emphasizes the importance of clinical and laboratory follow-up of HTLV-seroindeterminate individuals from endemic areas.

Authors+Show Affiliations

Department of Infectious Diseases, University of São Paulo, Brazil.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19188090

Citation

Costa, José Marcos Pereira, and Aluisio Cotrim Segurado. "Molecular Evidence of Human T-cell Lymphotropic Virus Types 1 and 2 (HTLV-1 and HTLV-2) Infections in HTLV Seroindeterminate Individuals From São Paulo, Brazil." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 44, no. 3, 2009, pp. 185-9.
Costa JM, Segurado AC. Molecular evidence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from São Paulo, Brazil. J Clin Virol. 2009;44(3):185-9.
Costa, J. M., & Segurado, A. C. (2009). Molecular evidence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from São Paulo, Brazil. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 44(3), 185-9. https://doi.org/10.1016/j.jcv.2008.12.015
Costa JM, Segurado AC. Molecular Evidence of Human T-cell Lymphotropic Virus Types 1 and 2 (HTLV-1 and HTLV-2) Infections in HTLV Seroindeterminate Individuals From São Paulo, Brazil. J Clin Virol. 2009;44(3):185-9. PubMed PMID: 19188090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular evidence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from São Paulo, Brazil. AU - Costa,José Marcos Pereira, AU - Segurado,Aluisio Cotrim, Y1 - 2009/02/01/ PY - 2008/08/13/received PY - 2008/12/11/revised PY - 2008/12/20/accepted PY - 2009/2/4/entrez PY - 2009/2/4/pubmed PY - 2009/4/14/medline SP - 185 EP - 9 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 44 IS - 3 N2 - BACKGROUND: Using enzyme immunoassays and Western blot (Wb) tests, HTLV serodiagnosis yields indeterminate results in a significant number of cases. OBJECTIVE: To determine the prevalence of HTLV infection among HTLV-seroindeterminate individuals. STUDY DESIGN: We studied peripheral blood mononuclear cells from 65 anti-HTLV Wb-seroindeterminate individuals by attempting to amplify proviral DNA sequences (tax and pol) to identify HTLV-1 and HTLV-2 infections. RESULTS: These 65 specimens exhibited predominantly (43%) anti-HTLV antibodies to gag-coded antigens in the absence of anti-p24 on Wb analysis. Tax proviral sequences were detected in 6 (9.2%) samples. According to restricted fragment polymorphism analysis (RFLP), we identified HTLV-1 proviral DNA in 4 samples, HTLV-2 in one and sequences from both in another. Nested PCR for the pol region was positive in 3 (4.6%) specimens, which were also positive for tax sequences. After hybridization HTLV-1 infection was confirmed in 2 samples (3.1%) and HTLV-2 in another (1.5%). Detection of a single HTLV DNA sequence may be due to infection by defective provirus, but its significance remains undefined. In this cohort, no Wb reactivity pattern was predictive of proviral detection. HTLV-1 infection was demonstrated in an individual who had Wb reactivity to gag-coded antigens only. CONCLUSIONS: This emphasizes the importance of clinical and laboratory follow-up of HTLV-seroindeterminate individuals from endemic areas. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/19188090/Molecular_evidence_of_human_T_cell_lymphotropic_virus_types_1_and_2__HTLV_1_and_HTLV_2__infections_in_HTLV_seroindeterminate_individuals_from_São_Paulo_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(09)00002-X DB - PRIME DP - Unbound Medicine ER -