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Modulation in reproductive tissue redox profile in sexually receptive female rats after short-term exposure to male chemical cues.
Chem Senses. 2009 May; 34(4):317-23.CS

Abstract

It is well known that antioxidants play an important role in sperm fertility, but there is no data on the literature regarding the effect of male chemical cues in the antioxidant defenses of the female reproductive tract. Here, we evaluated oxidative parameters in ovaries and uterus of virgin female rats isolated from contact to males and exposed only to male-soiled bedding (MSB). Four-month-old Wistar (regular 4-day cyclic) virgin female rats were utilized from proestrus to estrus phase of the reproductive cycle for experimental exposure. In an isolated room, female rats were exposed for 90 min to MSB. For biochemical assays, female rats were killed by decapitation at 30, 90, 180, and 240 min after the end of exposure, and the ovaries and uterus were removed for further analysis. Antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase), the nonenzymatic antioxidant potential (total radical-trapping antioxidant parameter), and the oxidative damage parameters (thiobarbituric acid-reactive species and carbonyl content) were analyzed. We observed an increase in the nonenzymatic antioxidant potential and diminished free radical oxidative damage in uterine tissue, 30 and 90 min after exposure. Furthermore, in ovaries, enzymatic defenses were modulated distinctly along the 240 min after exposure. MSB exposure modulates the antioxidant profile in ovaries and uterus of receptive female rats. It is possible that the modifications in the oxidative profile of the female genital tract may have important implications in the process of fertilization.

Authors+Show Affiliations

Centro de Estudos em Estresse Oxidativo (Lab. 32), Depto Bioquímica, Universidade Federal do Rio Grande do Sul. Av. Ramiro Barcelos, 2600 Anexo, CEP 90035-003, Porto Alegre, RS, Brazil. guibehr@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19188278

Citation

Behr, Guilherme Antônio, et al. "Modulation in Reproductive Tissue Redox Profile in Sexually Receptive Female Rats After Short-term Exposure to Male Chemical Cues." Chemical Senses, vol. 34, no. 4, 2009, pp. 317-23.
Behr GA, da Motta LL, de Oliveira MR, et al. Modulation in reproductive tissue redox profile in sexually receptive female rats after short-term exposure to male chemical cues. Chem Senses. 2009;34(4):317-23.
Behr, G. A., da Motta, L. L., de Oliveira, M. R., Oliveira, M. W., Gelain, D. P., & Moreira, J. C. (2009). Modulation in reproductive tissue redox profile in sexually receptive female rats after short-term exposure to male chemical cues. Chemical Senses, 34(4), 317-23. https://doi.org/10.1093/chemse/bjp004
Behr GA, et al. Modulation in Reproductive Tissue Redox Profile in Sexually Receptive Female Rats After Short-term Exposure to Male Chemical Cues. Chem Senses. 2009;34(4):317-23. PubMed PMID: 19188278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation in reproductive tissue redox profile in sexually receptive female rats after short-term exposure to male chemical cues. AU - Behr,Guilherme Antônio, AU - da Motta,Leonardo Lisbôa, AU - de Oliveira,Marcos Roberto, AU - Oliveira,Max William Soares, AU - Gelain,Daniel Pens, AU - Moreira,José Cláudio Fonseca, Y1 - 2009/02/02/ PY - 2009/2/4/entrez PY - 2009/2/4/pubmed PY - 2009/6/12/medline SP - 317 EP - 23 JF - Chemical senses JO - Chem. Senses VL - 34 IS - 4 N2 - It is well known that antioxidants play an important role in sperm fertility, but there is no data on the literature regarding the effect of male chemical cues in the antioxidant defenses of the female reproductive tract. Here, we evaluated oxidative parameters in ovaries and uterus of virgin female rats isolated from contact to males and exposed only to male-soiled bedding (MSB). Four-month-old Wistar (regular 4-day cyclic) virgin female rats were utilized from proestrus to estrus phase of the reproductive cycle for experimental exposure. In an isolated room, female rats were exposed for 90 min to MSB. For biochemical assays, female rats were killed by decapitation at 30, 90, 180, and 240 min after the end of exposure, and the ovaries and uterus were removed for further analysis. Antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase), the nonenzymatic antioxidant potential (total radical-trapping antioxidant parameter), and the oxidative damage parameters (thiobarbituric acid-reactive species and carbonyl content) were analyzed. We observed an increase in the nonenzymatic antioxidant potential and diminished free radical oxidative damage in uterine tissue, 30 and 90 min after exposure. Furthermore, in ovaries, enzymatic defenses were modulated distinctly along the 240 min after exposure. MSB exposure modulates the antioxidant profile in ovaries and uterus of receptive female rats. It is possible that the modifications in the oxidative profile of the female genital tract may have important implications in the process of fertilization. SN - 1464-3553 UR - https://www.unboundmedicine.com/medline/citation/19188278/Modulation_in_reproductive_tissue_redox_profile_in_sexually_receptive_female_rats_after_short_term_exposure_to_male_chemical_cues_ L2 - https://academic.oup.com/chemse/article-lookup/doi/10.1093/chemse/bjp004 DB - PRIME DP - Unbound Medicine ER -