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A metabolically stable analogue of anandamide, Met-F-AEA, inhibits human thyroid carcinoma cell lines by activation of apoptosis.
Invest New Drugs 2010; 28(2):115-23IN

Abstract

The active components of Cannabis sativa and their derivatives produce a wide spectrum of effects, some of which may have clinical application. The discovery of specific cannabinoid receptors and a family of endogenous ligands of those receptors has attracted much attention to cannabinoids as agents capable of controlling the decision of cells to survive or die. We analysed the effects exerted by 2-methyl-2'-F-anandamide (Met-F-AEA), a metabolically stable analogue of anandamide, and observed a growth inhibition in cell lines derived from thyroid carcinomas. Growth inhibition was associated with a high level of CB1 receptor expression, suggesting that the cytotoxic effect is due to interaction with the CB1 receptor. This phenomenon was associated with activation of the protein, p53, an increased apoptotic rate, and expression of p21(CIP1/WAF1). This study provides new insights into the mechanism of Met-F-AEA action, and could have significance in providing a basis for the management of thyroid carcinoma.

Authors+Show Affiliations

Department of Structural and Functional Biology, University of Naples Federico II, 80126 Naples, Italy. cozzolino1@interfree.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19189054

Citation

Cozzolino, Rosanna, et al. "A Metabolically Stable Analogue of Anandamide, Met-F-AEA, Inhibits Human Thyroid Carcinoma Cell Lines By Activation of Apoptosis." Investigational New Drugs, vol. 28, no. 2, 2010, pp. 115-23.
Cozzolino R, Calì G, Bifulco M, et al. A metabolically stable analogue of anandamide, Met-F-AEA, inhibits human thyroid carcinoma cell lines by activation of apoptosis. Invest New Drugs. 2010;28(2):115-23.
Cozzolino, R., Calì, G., Bifulco, M., & Laccetti, P. (2010). A metabolically stable analogue of anandamide, Met-F-AEA, inhibits human thyroid carcinoma cell lines by activation of apoptosis. Investigational New Drugs, 28(2), pp. 115-23. doi:10.1007/s10637-009-9221-0.
Cozzolino R, et al. A Metabolically Stable Analogue of Anandamide, Met-F-AEA, Inhibits Human Thyroid Carcinoma Cell Lines By Activation of Apoptosis. Invest New Drugs. 2010;28(2):115-23. PubMed PMID: 19189054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A metabolically stable analogue of anandamide, Met-F-AEA, inhibits human thyroid carcinoma cell lines by activation of apoptosis. AU - Cozzolino,Rosanna, AU - Calì,Gaetano, AU - Bifulco,Maurizio, AU - Laccetti,Paolo, Y1 - 2009/02/03/ PY - 2008/11/20/received PY - 2009/01/22/accepted PY - 2009/2/4/entrez PY - 2009/2/4/pubmed PY - 2010/6/30/medline SP - 115 EP - 23 JF - Investigational new drugs JO - Invest New Drugs VL - 28 IS - 2 N2 - The active components of Cannabis sativa and their derivatives produce a wide spectrum of effects, some of which may have clinical application. The discovery of specific cannabinoid receptors and a family of endogenous ligands of those receptors has attracted much attention to cannabinoids as agents capable of controlling the decision of cells to survive or die. We analysed the effects exerted by 2-methyl-2'-F-anandamide (Met-F-AEA), a metabolically stable analogue of anandamide, and observed a growth inhibition in cell lines derived from thyroid carcinomas. Growth inhibition was associated with a high level of CB1 receptor expression, suggesting that the cytotoxic effect is due to interaction with the CB1 receptor. This phenomenon was associated with activation of the protein, p53, an increased apoptotic rate, and expression of p21(CIP1/WAF1). This study provides new insights into the mechanism of Met-F-AEA action, and could have significance in providing a basis for the management of thyroid carcinoma. SN - 1573-0646 UR - https://www.unboundmedicine.com/medline/citation/19189054/abstract/A_metabolically_stable_analogue_of_anandamide_Met_F_AEA_inhibits_human_thyroid_carcinoma_cell_lines_by_activation_of_apoptosis_ DB - PRIME DP - Unbound Medicine ER -