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Alveolar macrophage subpopulations in bronchoalveolar lavage and induced sputum of asthmatic and control subjects.
J Asthma. 2009 Feb; 46(1):1-8.JA

Abstract

BACKGROUND

Alveolar macrophages (AM) are the most numerous immune cells in the airways and are involved in the immunological homeostasis of the lung. Intriguingly, their role in asthma remains unclear probably, in part, because of their heterogeneity.

OBJECTIVE

To characterize AM population from bronchoalveolar lavage (BAL) and induced sputum (IS) of asthmatic and normal subjects using specific biomarkers.

METHODS

Non-asthmatic non-allergic and allergic mild asthmatic subjects were recruited for this study. AM were obtained from BAL and IS and cytospins were prepared. Immunocytochemistry was performed for nine cellular markers (CD68, RFD7, CD14, CD11b, CD83, CD64, CD80, CD86, and FIZZ1).

RESULTS

Asthmatic subjects had more AM RFD7(+) in BAL compared with IS, whereas control subjects had more AM RFD7(+) in IS than in BAL. Consequently, there was an increased number of AM RFD7(+) in BAL of asthmatic subjects compared with BAL of control subjects. AM CD11b(+) was higher in BAL than in IS in both groups. The expression of FIZZ1, marker of macrophage alternative activation, was similar in asthmatic and normal subjects.

CONCLUSION

The expression of cellular markers on AM differs according to their localization in the lung. Subpopulations of AM may contribute to the inflammatory profile observed in asthmatic subjects.

Authors+Show Affiliations

Centre de Recherche de l'Hopital Laval, Institut Universitaire de Cardiologie et de Pneumologie de l'Universite Laval, Quebec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19191129

Citation

St-Laurent, Julie, et al. "Alveolar Macrophage Subpopulations in Bronchoalveolar Lavage and Induced Sputum of Asthmatic and Control Subjects." The Journal of Asthma : Official Journal of the Association for the Care of Asthma, vol. 46, no. 1, 2009, pp. 1-8.
St-Laurent J, Turmel V, Boulet LP, et al. Alveolar macrophage subpopulations in bronchoalveolar lavage and induced sputum of asthmatic and control subjects. J Asthma. 2009;46(1):1-8.
St-Laurent, J., Turmel, V., Boulet, L. P., & Bissonnette, E. (2009). Alveolar macrophage subpopulations in bronchoalveolar lavage and induced sputum of asthmatic and control subjects. The Journal of Asthma : Official Journal of the Association for the Care of Asthma, 46(1), 1-8. https://doi.org/10.1080/02770900802444211
St-Laurent J, et al. Alveolar Macrophage Subpopulations in Bronchoalveolar Lavage and Induced Sputum of Asthmatic and Control Subjects. J Asthma. 2009;46(1):1-8. PubMed PMID: 19191129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alveolar macrophage subpopulations in bronchoalveolar lavage and induced sputum of asthmatic and control subjects. AU - St-Laurent,Julie, AU - Turmel,Veronique, AU - Boulet,Louis-Philippe, AU - Bissonnette,Elyse, PY - 2009/2/5/entrez PY - 2009/2/5/pubmed PY - 2009/2/21/medline SP - 1 EP - 8 JF - The Journal of asthma : official journal of the Association for the Care of Asthma JO - J Asthma VL - 46 IS - 1 N2 - BACKGROUND: Alveolar macrophages (AM) are the most numerous immune cells in the airways and are involved in the immunological homeostasis of the lung. Intriguingly, their role in asthma remains unclear probably, in part, because of their heterogeneity. OBJECTIVE: To characterize AM population from bronchoalveolar lavage (BAL) and induced sputum (IS) of asthmatic and normal subjects using specific biomarkers. METHODS: Non-asthmatic non-allergic and allergic mild asthmatic subjects were recruited for this study. AM were obtained from BAL and IS and cytospins were prepared. Immunocytochemistry was performed for nine cellular markers (CD68, RFD7, CD14, CD11b, CD83, CD64, CD80, CD86, and FIZZ1). RESULTS: Asthmatic subjects had more AM RFD7(+) in BAL compared with IS, whereas control subjects had more AM RFD7(+) in IS than in BAL. Consequently, there was an increased number of AM RFD7(+) in BAL of asthmatic subjects compared with BAL of control subjects. AM CD11b(+) was higher in BAL than in IS in both groups. The expression of FIZZ1, marker of macrophage alternative activation, was similar in asthmatic and normal subjects. CONCLUSION: The expression of cellular markers on AM differs according to their localization in the lung. Subpopulations of AM may contribute to the inflammatory profile observed in asthmatic subjects. SN - 1532-4303 UR - https://www.unboundmedicine.com/medline/citation/19191129/Alveolar_macrophage_subpopulations_in_bronchoalveolar_lavage_and_induced_sputum_of_asthmatic_and_control_subjects_ L2 - https://www.tandfonline.com/doi/full/10.1080/02770900802444211 DB - PRIME DP - Unbound Medicine ER -