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Oral ulcers in HIV-positive Peruvian patients: an immunohistochemical and in situ hybridization study.
J Oral Pathol Med. 2009 Jan; 38(1):120-5.JO

Abstract

BACKGROUND

This study describes the histopathological, immunohistochemical (IHC) and in situ hybridization (ISH) data of 25 cases of oral ulcers in HIV-positive patients, with clinical and microscopical features similar to ulcers not otherwise specified (NOS)/necrotizing ulcerative stomatitis (NUS).

METHODS

Sex, age and clinical history were obtained from the clinical records. Histological analysis for H&E, Gomori-Grocott and Ziehl-Neelsen stains, IHC analysis to detect infectious agents and to characterize inflammatory cellular infiltrate, and ISH for cytomegalovirus (CMV) and EBER1/2 were performed.

RESULTS

Twenty-one patients were men and four were women (mean age of 34.6 years). The tongue was preferentially affected. Microscopically, the lesions showed extensive necrosis, leukocytoclasia, vasculitis with luminal fibrin clots and an intense inflammatory cellular infiltrate predominated by CD68(+) atypical large cells, normal-sized and crescent-shaped nuclei macrophages, interspersed by CD8(+) T lymphocytes. Mast cells were also observed in all samples studied. CD4(+) T lymphocytes, CD20(+) B lymphocytes and VS38c(+) plasma cells were practically absent. CMV and EBER1/2 were identified in scarce cells of 3 and 16 of 25 cases respectively.

CONCLUSION

These results show that CD68(+) macrophages, followed by CD8(+) T lymphocytes, were the predominant inflammatory cells, indicating they are relevant to the pathogenesis of the ulcers, possibly reflecting an abnormal immune response in the oral mucosa. The clinicopathological and immunoprofile features of the present cases are similar to NOS ulcers/NUS in HIV-positive patients.

Authors+Show Affiliations

Department of Oral Pathology, Faculty of Stomatology, University Cayetano Heredia, Lima, Perú. jorgeesquiche@yahoo.com.brNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19192057

Citation

Delgado, W A., et al. "Oral Ulcers in HIV-positive Peruvian Patients: an Immunohistochemical and in Situ Hybridization Study." Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, vol. 38, no. 1, 2009, pp. 120-5.
Delgado WA, Almeida OP, Vargas PA, et al. Oral ulcers in HIV-positive Peruvian patients: an immunohistochemical and in situ hybridization study. J Oral Pathol Med. 2009;38(1):120-5.
Delgado, W. A., Almeida, O. P., Vargas, P. A., & León, J. E. (2009). Oral ulcers in HIV-positive Peruvian patients: an immunohistochemical and in situ hybridization study. Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 38(1), 120-5. https://doi.org/10.1111/j.1600-0714.2008.00714.x
Delgado WA, et al. Oral Ulcers in HIV-positive Peruvian Patients: an Immunohistochemical and in Situ Hybridization Study. J Oral Pathol Med. 2009;38(1):120-5. PubMed PMID: 19192057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral ulcers in HIV-positive Peruvian patients: an immunohistochemical and in situ hybridization study. AU - Delgado,W A, AU - Almeida,O P, AU - Vargas,P A, AU - León,J E, PY - 2009/2/5/entrez PY - 2009/2/5/pubmed PY - 2009/5/23/medline SP - 120 EP - 5 JF - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology JO - J Oral Pathol Med VL - 38 IS - 1 N2 - BACKGROUND: This study describes the histopathological, immunohistochemical (IHC) and in situ hybridization (ISH) data of 25 cases of oral ulcers in HIV-positive patients, with clinical and microscopical features similar to ulcers not otherwise specified (NOS)/necrotizing ulcerative stomatitis (NUS). METHODS: Sex, age and clinical history were obtained from the clinical records. Histological analysis for H&E, Gomori-Grocott and Ziehl-Neelsen stains, IHC analysis to detect infectious agents and to characterize inflammatory cellular infiltrate, and ISH for cytomegalovirus (CMV) and EBER1/2 were performed. RESULTS: Twenty-one patients were men and four were women (mean age of 34.6 years). The tongue was preferentially affected. Microscopically, the lesions showed extensive necrosis, leukocytoclasia, vasculitis with luminal fibrin clots and an intense inflammatory cellular infiltrate predominated by CD68(+) atypical large cells, normal-sized and crescent-shaped nuclei macrophages, interspersed by CD8(+) T lymphocytes. Mast cells were also observed in all samples studied. CD4(+) T lymphocytes, CD20(+) B lymphocytes and VS38c(+) plasma cells were practically absent. CMV and EBER1/2 were identified in scarce cells of 3 and 16 of 25 cases respectively. CONCLUSION: These results show that CD68(+) macrophages, followed by CD8(+) T lymphocytes, were the predominant inflammatory cells, indicating they are relevant to the pathogenesis of the ulcers, possibly reflecting an abnormal immune response in the oral mucosa. The clinicopathological and immunoprofile features of the present cases are similar to NOS ulcers/NUS in HIV-positive patients. SN - 1600-0714 UR - https://www.unboundmedicine.com/medline/citation/19192057/Oral_ulcers_in_HIV_positive_Peruvian_patients:_an_immunohistochemical_and_in_situ_hybridization_study_ L2 - https://doi.org/10.1111/j.1600-0714.2008.00714.x DB - PRIME DP - Unbound Medicine ER -