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Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma.
Respirology. 2009 Mar; 14(2):187-94.R

Abstract

BACKGROUND AND OBJECTIVE

A forkhead/winged-helix family transcriptional repressor, Foxp3, is highly expressed on CD4(+)CD25(+) T regulatory cells. The role of Foxp3(+)CD4(+)CD25(+) T regulatory cells in asthma remains to be elucidated. Using mouse models and peripheral blood mononuclear cells (PBMC) from subjects with allergic asthma, we aimed to explore whether Foxp3(+)CD4(+)CD25(+) T regulatory cells associate with asthma phenotypes.

METHODS

Foxp3(+)CD4(+)CD25(+) T cells were detected by FACS and the correlation between the frequency of Foxp3(+)CD4(+)CD25(+) T cells and asthma phenotypes was assessed.

RESULTS

The frequency of Foxp3(+)CD4(+)CD25(+) T cells among total CD4(+)CD25(+) T cells in the lungs showed an inverse correlation with eosinophilic inflammation in BALB/c, A/J and C57BL/6 strains. In addition, the frequency of Foxp3(+)CD4(+)CD25(+) T cells was inversely correlated with BHR and allergen-specific IgE levels in the serum of A/J mice. In BALB/c mice, the frequency of Foxp3(+)CD4(+)CD25(+) T cells correlated with the level of IL-10 in BAL fluid. The inverse correlation between the frequency of Foxp3(+)CD4(+)CD25(+) T cells and eosinophilic inflammation disappeared when mice were treated with anti-IL-10 receptor mAb during allergen challenge. Interestingly, intracellular cytokine staining of lung cells revealed that IL-10 was predominantly produced by Foxp3(-)CD4(+)CD25(+) T cells. The frequency of Foxp3(+)CD4(+)CD25(+) T cells among total CD4(+)CD25(+) T cells in PBMC of asthmatics was significantly lower than that of healthy subjects, although there was no significant correlation between the frequency of Foxp3(+)CD4(+)CD25(+) T cells and asthma severity.

CONCLUSIONS

These results suggest a role for lung Foxp3(+)CD4(+)CD25(+) T cells in the regulation of asthma phenotypes, presumably through an IL-10-mediated mechanism.

Authors+Show Affiliations

Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19192224

Citation

Matsumoto, Koichiro, et al. "Frequency of Foxp3+CD4CD25+ T Cells Is Associated With the Phenotypes of Allergic Asthma." Respirology (Carlton, Vic.), vol. 14, no. 2, 2009, pp. 187-94.
Matsumoto K, Inoue H, Fukuyama S, et al. Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma. Respirology. 2009;14(2):187-94.
Matsumoto, K., Inoue, H., Fukuyama, S., Kan-O, K., Eguchi-Tsuda, M., Matsumoto, T., Moriwaki, A., Nakano, T., & Nakanishi, Y. (2009). Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma. Respirology (Carlton, Vic.), 14(2), 187-94. https://doi.org/10.1111/j.1440-1843.2008.01472.x
Matsumoto K, et al. Frequency of Foxp3+CD4CD25+ T Cells Is Associated With the Phenotypes of Allergic Asthma. Respirology. 2009;14(2):187-94. PubMed PMID: 19192224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma. AU - Matsumoto,Koichiro, AU - Inoue,Hiromasa, AU - Fukuyama,Satoru, AU - Kan-O,Keiko, AU - Eguchi-Tsuda,Miyuki, AU - Matsumoto,Takafumi, AU - Moriwaki,Atsushi, AU - Nakano,Takako, AU - Nakanishi,Yoichi, Y1 - 2009/01/23/ PY - 2009/2/5/entrez PY - 2009/2/5/pubmed PY - 2009/7/10/medline SP - 187 EP - 94 JF - Respirology (Carlton, Vic.) JO - Respirology VL - 14 IS - 2 N2 - BACKGROUND AND OBJECTIVE: A forkhead/winged-helix family transcriptional repressor, Foxp3, is highly expressed on CD4(+)CD25(+) T regulatory cells. The role of Foxp3(+)CD4(+)CD25(+) T regulatory cells in asthma remains to be elucidated. Using mouse models and peripheral blood mononuclear cells (PBMC) from subjects with allergic asthma, we aimed to explore whether Foxp3(+)CD4(+)CD25(+) T regulatory cells associate with asthma phenotypes. METHODS: Foxp3(+)CD4(+)CD25(+) T cells were detected by FACS and the correlation between the frequency of Foxp3(+)CD4(+)CD25(+) T cells and asthma phenotypes was assessed. RESULTS: The frequency of Foxp3(+)CD4(+)CD25(+) T cells among total CD4(+)CD25(+) T cells in the lungs showed an inverse correlation with eosinophilic inflammation in BALB/c, A/J and C57BL/6 strains. In addition, the frequency of Foxp3(+)CD4(+)CD25(+) T cells was inversely correlated with BHR and allergen-specific IgE levels in the serum of A/J mice. In BALB/c mice, the frequency of Foxp3(+)CD4(+)CD25(+) T cells correlated with the level of IL-10 in BAL fluid. The inverse correlation between the frequency of Foxp3(+)CD4(+)CD25(+) T cells and eosinophilic inflammation disappeared when mice were treated with anti-IL-10 receptor mAb during allergen challenge. Interestingly, intracellular cytokine staining of lung cells revealed that IL-10 was predominantly produced by Foxp3(-)CD4(+)CD25(+) T cells. The frequency of Foxp3(+)CD4(+)CD25(+) T cells among total CD4(+)CD25(+) T cells in PBMC of asthmatics was significantly lower than that of healthy subjects, although there was no significant correlation between the frequency of Foxp3(+)CD4(+)CD25(+) T cells and asthma severity. CONCLUSIONS: These results suggest a role for lung Foxp3(+)CD4(+)CD25(+) T cells in the regulation of asthma phenotypes, presumably through an IL-10-mediated mechanism. SN - 1440-1843 UR - https://www.unboundmedicine.com/medline/citation/19192224/Frequency_of_Foxp3+CD4CD25+_T_cells_is_associated_with_the_phenotypes_of_allergic_asthma_ L2 - https://doi.org/10.1111/j.1440-1843.2008.01472.x DB - PRIME DP - Unbound Medicine ER -