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[Comparison of characteristics between glutaraldehyde- and genipin-crosslinked gelatin microspheres].

Abstract

OBJECTIVE

To compare the characteristics of gelatin microspheres crosslinked by glutaraldehyde (GA) or genipin (GP).

METHODS

Gelatin microspheres, prepared by the improved emulsified cold-condensation method, were crosslinked by GP and GA, respectively. After being dispersed in PBS, two kinds of microspheres with 60% degree of cross linking were compared in terms of morphology, swelling and degrading properties. rhBMP-2 were loaded into the GP and GA microspheres, and the encapsulation rate, drug loading and releasing capacity were measured; 100%, 50% and 25% leaching liquid of GP and GA microspheres were respectively cultured with rat osteoblast (DMEM group as the control), and cell proliferation was measured by MTT method to grade the cell cytotoxicity.

RESULTS

GP and GA microspheres were both spherical with the diameters of (78 +/- 18) microm and (65 +/- 10) microm, and there were no difference between both microspheres in drug loading and encapsulation rate. But, GP microspheres, with long degrading period (28 days) compared to GA microspheres (21 days), had better dispersibility, and swelling rate (89.0% +/- 4.8%), the percentage of cumulative drug releasing at 10 days (78.80% +/- 4.96%) were both lower than GA microsphere (118.0% +/- 7.6%, 90.50% +/- 5.12%). The percentages of drug loading of GP and GA were (921 +/- 73) and (965 +/- 62) ng/g, and the encapsulation rates were 88.5% +/- 2.1% and 89.7% +/- 1.8%; showing no significant difference (P > 0.05). The cell cytotoxicity of 100%, 50% and 25% leaching liquid of GP microspheres was all at the level I, but leaching liquid of GA microspheres with corresponding concentration were at the levels of III, III and II.

CONCLUSION

GP crosslinked gelatin microspheres are superior to GA crosslinked gelatin microspheres and can be widely used in tissue engineering field.

Authors+Show Affiliations

Department of Orthopeadics, General Hospital of Lanzhou Military Area Command of PLA, Lanzhou Gansu, 730050, P.R. China.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19192887

Citation

Li, Meng, et al. "[Comparison of Characteristics Between Glutaraldehyde- and Genipin-crosslinked Gelatin Microspheres]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 23, no. 1, 2009, pp. 87-91.
Li M, Liu X, Liu X. [Comparison of characteristics between glutaraldehyde- and genipin-crosslinked gelatin microspheres]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009;23(1):87-91.
Li, M., Liu, X., & Liu, X. (2009). [Comparison of characteristics between glutaraldehyde- and genipin-crosslinked gelatin microspheres]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 23(1), 87-91.
Li M, Liu X, Liu X. [Comparison of Characteristics Between Glutaraldehyde- and Genipin-crosslinked Gelatin Microspheres]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009;23(1):87-91. PubMed PMID: 19192887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparison of characteristics between glutaraldehyde- and genipin-crosslinked gelatin microspheres]. AU - Li,Meng, AU - Liu,Xudong, AU - Liu,Xingyan, PY - 2009/2/6/entrez PY - 2009/2/6/pubmed PY - 2009/6/13/medline SP - 87 EP - 91 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 23 IS - 1 N2 - OBJECTIVE: To compare the characteristics of gelatin microspheres crosslinked by glutaraldehyde (GA) or genipin (GP). METHODS: Gelatin microspheres, prepared by the improved emulsified cold-condensation method, were crosslinked by GP and GA, respectively. After being dispersed in PBS, two kinds of microspheres with 60% degree of cross linking were compared in terms of morphology, swelling and degrading properties. rhBMP-2 were loaded into the GP and GA microspheres, and the encapsulation rate, drug loading and releasing capacity were measured; 100%, 50% and 25% leaching liquid of GP and GA microspheres were respectively cultured with rat osteoblast (DMEM group as the control), and cell proliferation was measured by MTT method to grade the cell cytotoxicity. RESULTS: GP and GA microspheres were both spherical with the diameters of (78 +/- 18) microm and (65 +/- 10) microm, and there were no difference between both microspheres in drug loading and encapsulation rate. But, GP microspheres, with long degrading period (28 days) compared to GA microspheres (21 days), had better dispersibility, and swelling rate (89.0% +/- 4.8%), the percentage of cumulative drug releasing at 10 days (78.80% +/- 4.96%) were both lower than GA microsphere (118.0% +/- 7.6%, 90.50% +/- 5.12%). The percentages of drug loading of GP and GA were (921 +/- 73) and (965 +/- 62) ng/g, and the encapsulation rates were 88.5% +/- 2.1% and 89.7% +/- 1.8%; showing no significant difference (P > 0.05). The cell cytotoxicity of 100%, 50% and 25% leaching liquid of GP microspheres was all at the level I, but leaching liquid of GA microspheres with corresponding concentration were at the levels of III, III and II. CONCLUSION: GP crosslinked gelatin microspheres are superior to GA crosslinked gelatin microspheres and can be widely used in tissue engineering field. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/19192887/[Comparison_of_characteristics_between_glutaraldehyde__and_genipin_crosslinked_gelatin_microspheres]_ DB - PRIME DP - Unbound Medicine ER -