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In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds.
J Agric Food Chem. 2009 Feb 25; 57(4):1247-52.JA

Abstract

In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.

Authors+Show Affiliations

REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19192972

Citation

Taveira, Marcos, et al. "In Vitro Cultures of Brassica Oleracea L. Var. Costata DC: Potential Plant Bioreactor for Antioxidant Phenolic Compounds." Journal of Agricultural and Food Chemistry, vol. 57, no. 4, 2009, pp. 1247-52.
Taveira M, Pereira DM, Sousa C, et al. In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds. J Agric Food Chem. 2009;57(4):1247-52.
Taveira, M., Pereira, D. M., Sousa, C., Ferreres, F., Andrade, P. B., Martins, A., Pereira, J. A., & Valentão, P. (2009). In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds. Journal of Agricultural and Food Chemistry, 57(4), 1247-52. https://doi.org/10.1021/jf803496x
Taveira M, et al. In Vitro Cultures of Brassica Oleracea L. Var. Costata DC: Potential Plant Bioreactor for Antioxidant Phenolic Compounds. J Agric Food Chem. 2009 Feb 25;57(4):1247-52. PubMed PMID: 19192972.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds. AU - Taveira,Marcos, AU - Pereira,David M, AU - Sousa,Carla, AU - Ferreres,Federico, AU - Andrade,Paula B, AU - Martins,Anabela, AU - Pereira,José A, AU - Valentão,Patrícia, PY - 2009/2/6/entrez PY - 2009/2/6/pubmed PY - 2009/4/22/medline SP - 1247 EP - 52 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 4 N2 - In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19192972/In_vitro_cultures_of_Brassica_oleracea_L__var__costata_DC:_potential_plant_bioreactor_for_antioxidant_phenolic_compounds_ L2 - https://doi.org/10.1021/jf803496x DB - PRIME DP - Unbound Medicine ER -