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Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo.
J Cell Sci. 2009 Feb 15; 122(Pt 4):481-8.JC

Abstract

Muscle regulatory factors activate myogenesis in all vertebrates, but their role has been studied in great detail only in the mouse embryo, where all but myogenin--Myod, Myf5 and Mrf4--are sufficient to activate (albeit not completely) skeletal myogenesis. In the zebrafish embryo, myod and myf5 are required for induction of myogenesis because their simultaneous ablation prevents muscle development. Here we show that mrf4 but not myog can fully rescue myogenesis in the myod/myf5 double morphant via a selective and robust activation of myod, in keeping with its chromatin-remodelling function in vitro. Rescue does not happen spontaneously, because the gene, unlike that in the mouse embryo, is expressed only at the onset of muscle differentiation, Moreover, because of the transient nature of morpholino inhibition, we were able to investigate how myogenesis occurs in the absence of a myotome. We report that in the complete absence of a myotome, subsequent myogenesis is abolished, whereas myogenesis does proceed, albeit abnormally, when the morpholino inhibition was not complete. Therefore our data also show that the early myotome is essential for subsequent skeletal muscle differentiation and patterning in the zebrafish.

Authors+Show Affiliations

Stem Cell Research Institute, DiBiT, San Raffaele Scientific Institute, 58 via Olgettina, 20132 Milan, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19193870

Citation

Schnapp, Esther, et al. "Induced Early Expression of Mrf4 but Not Myog Rescues Myogenesis in the Myod/myf5 Double-morphant Zebrafish Embryo." Journal of Cell Science, vol. 122, no. Pt 4, 2009, pp. 481-8.
Schnapp E, Pistocchi AS, Karampetsou E, et al. Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo. J Cell Sci. 2009;122(Pt 4):481-8.
Schnapp, E., Pistocchi, A. S., Karampetsou, E., Foglia, E., Lamia, C. L., Cotelli, F., & Cossu, G. (2009). Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo. Journal of Cell Science, 122(Pt 4), 481-8. https://doi.org/10.1242/jcs.038356
Schnapp E, et al. Induced Early Expression of Mrf4 but Not Myog Rescues Myogenesis in the Myod/myf5 Double-morphant Zebrafish Embryo. J Cell Sci. 2009 Feb 15;122(Pt 4):481-8. PubMed PMID: 19193870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo. AU - Schnapp,Esther, AU - Pistocchi,Anna Silvia, AU - Karampetsou,Evangelia, AU - Foglia,Efrem, AU - Lamia,Carla Lora, AU - Cotelli,Franco, AU - Cossu,Giulio, PY - 2009/2/6/entrez PY - 2009/2/6/pubmed PY - 2009/4/22/medline SP - 481 EP - 8 JF - Journal of cell science JO - J Cell Sci VL - 122 IS - Pt 4 N2 - Muscle regulatory factors activate myogenesis in all vertebrates, but their role has been studied in great detail only in the mouse embryo, where all but myogenin--Myod, Myf5 and Mrf4--are sufficient to activate (albeit not completely) skeletal myogenesis. In the zebrafish embryo, myod and myf5 are required for induction of myogenesis because their simultaneous ablation prevents muscle development. Here we show that mrf4 but not myog can fully rescue myogenesis in the myod/myf5 double morphant via a selective and robust activation of myod, in keeping with its chromatin-remodelling function in vitro. Rescue does not happen spontaneously, because the gene, unlike that in the mouse embryo, is expressed only at the onset of muscle differentiation, Moreover, because of the transient nature of morpholino inhibition, we were able to investigate how myogenesis occurs in the absence of a myotome. We report that in the complete absence of a myotome, subsequent myogenesis is abolished, whereas myogenesis does proceed, albeit abnormally, when the morpholino inhibition was not complete. Therefore our data also show that the early myotome is essential for subsequent skeletal muscle differentiation and patterning in the zebrafish. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/19193870/Induced_early_expression_of_mrf4_but_not_myog_rescues_myogenesis_in_the_myod/myf5_double_morphant_zebrafish_embryo_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=19193870 DB - PRIME DP - Unbound Medicine ER -