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Regio-distribution of stearic acid is not conserved in chylomicrons after ingestion of randomised, stearic acid-rich fat in a single meal.
J Nutr Biochem. 2009 Nov; 20(11):909-15.JN

Abstract

Stearic acid from conventional food is well absorbed, but the fate of synthetic randomized stearic acid in fat absorption and subsequent metabolism is unclear. In this study, we examined the postprandial triglyceridemia following an ingestion of randomized stearic acid-rich fat. Following a 12-h fast, nine healthy young males ate a hamburger meal with 16.7 g of stearic acid (30% in triacylglycerol (TAG) sn-2 position, fully randomized). Postprandial blood samples were collected for 450 min, and the stearic acid content in chylomicron (CM, Svedberg flotation rate >400) TAG and the proportion of stearic acid in the sn-2 position were measured by tandem mass spectrometry at peak (180 min) and late (360 min) triglyceridemia. Of all stearic acid in CM TAG, 23% and 22% were in the sn-2 position at peak and late triglyceridemia (P<.004 and P<.001, respectively). This suggests a 68% and 62% conservation of sn-2 stearic acid, respectively. Peak postprandial TAG concentration and incremental area under the TAG curve showed a higher correlation with the fasting CM TAG (r=0.88, P<.01 and r=0.72, P<.05, respectively) than with total fasting plasma TAG (r=0.73, P<.05 and r=0.24, nonsignificant, respectively). In an earlier study, we showed that the absorption efficiency of the stearic acid of the meal was normal, with only marginal amounts of mainly sn-1,3 stearic acid found in the feces. In conclusion, we showed that sn-2 stearic acid is underrepresented in the postprandial CM TAG following an ingestion of fully randomized fat.

Authors+Show Affiliations

Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland. saska.tuomasjukka@utu.fiNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19195862

Citation

Tuomasjukka, Saska S., et al. "Regio-distribution of Stearic Acid Is Not Conserved in Chylomicrons After Ingestion of Randomised, Stearic Acid-rich Fat in a Single Meal." The Journal of Nutritional Biochemistry, vol. 20, no. 11, 2009, pp. 909-15.
Tuomasjukka SS, Viitanen MH, Kallio HP. Regio-distribution of stearic acid is not conserved in chylomicrons after ingestion of randomised, stearic acid-rich fat in a single meal. J Nutr Biochem. 2009;20(11):909-15.
Tuomasjukka, S. S., Viitanen, M. H., & Kallio, H. P. (2009). Regio-distribution of stearic acid is not conserved in chylomicrons after ingestion of randomised, stearic acid-rich fat in a single meal. The Journal of Nutritional Biochemistry, 20(11), 909-15. https://doi.org/10.1016/j.jnutbio.2008.10.005
Tuomasjukka SS, Viitanen MH, Kallio HP. Regio-distribution of Stearic Acid Is Not Conserved in Chylomicrons After Ingestion of Randomised, Stearic Acid-rich Fat in a Single Meal. J Nutr Biochem. 2009;20(11):909-15. PubMed PMID: 19195862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regio-distribution of stearic acid is not conserved in chylomicrons after ingestion of randomised, stearic acid-rich fat in a single meal. AU - Tuomasjukka,Saska S, AU - Viitanen,Matti H, AU - Kallio,Heikki P, Y1 - 2009/02/05/ PY - 2008/06/16/received PY - 2008/10/01/revised PY - 2008/10/02/accepted PY - 2009/2/7/entrez PY - 2009/2/7/pubmed PY - 2010/1/20/medline SP - 909 EP - 15 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 20 IS - 11 N2 - Stearic acid from conventional food is well absorbed, but the fate of synthetic randomized stearic acid in fat absorption and subsequent metabolism is unclear. In this study, we examined the postprandial triglyceridemia following an ingestion of randomized stearic acid-rich fat. Following a 12-h fast, nine healthy young males ate a hamburger meal with 16.7 g of stearic acid (30% in triacylglycerol (TAG) sn-2 position, fully randomized). Postprandial blood samples were collected for 450 min, and the stearic acid content in chylomicron (CM, Svedberg flotation rate >400) TAG and the proportion of stearic acid in the sn-2 position were measured by tandem mass spectrometry at peak (180 min) and late (360 min) triglyceridemia. Of all stearic acid in CM TAG, 23% and 22% were in the sn-2 position at peak and late triglyceridemia (P<.004 and P<.001, respectively). This suggests a 68% and 62% conservation of sn-2 stearic acid, respectively. Peak postprandial TAG concentration and incremental area under the TAG curve showed a higher correlation with the fasting CM TAG (r=0.88, P<.01 and r=0.72, P<.05, respectively) than with total fasting plasma TAG (r=0.73, P<.05 and r=0.24, nonsignificant, respectively). In an earlier study, we showed that the absorption efficiency of the stearic acid of the meal was normal, with only marginal amounts of mainly sn-1,3 stearic acid found in the feces. In conclusion, we showed that sn-2 stearic acid is underrepresented in the postprandial CM TAG following an ingestion of fully randomized fat. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/19195862/Regio_distribution_of_stearic_acid_is_not_conserved_in_chylomicrons_after_ingestion_of_randomised_stearic_acid_rich_fat_in_a_single_meal_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(08)00224-6 DB - PRIME DP - Unbound Medicine ER -