Tags

Type your tag names separated by a space and hit enter

Multiclass analysis of antibiotic residues in honey by ultraperformance liquid chromatography-tandem mass spectrometry.
J Agric Food Chem. 2009 Mar 11; 57(5):1760-7.JA

Abstract

A method has been developed and validated for the simultaneous analysis of different veterinary drug residues (macrolides, tetracyclines, quinolones, and sulfonamides) in honey. Honey samples were dissolved with Na(2)EDTA, and veterinary residues were extracted from the supernatant by solid-phase extraction (SPE), using OASIS HLB cartridges. The separation and determination was carried out by ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS), using an electrospay ionization source (ESI) in positive mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of sensitivity and specificity. The method was validated, and mean recoveries were evaluated at three concentration levels (10, 50, and 100 microg/kg), ranging from 70 to 120% except for doxycycline, erythromycin, and tylmicosin with recovery higher than 50% at the three levels assayed. Relative standard deviations (RSDs) of the recoveries were less than 20% within the intraday precision and less than 25% within the interday precision. The limits of quantification (LOQs) were always lower than 4 microg/kg. The developed procedure was applied to 16 honey samples, and erythromycin, sarafloxacin, and tylosin were found in a few samples.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Vidal JL
Group "Analytical Chemistry of Contaminants", Department of Analytical Chemistry, Almeria University, E-04071 Almeria, Spain.
Aguilera-Luiz Mdel M
No affiliation info available
Romero-González R
No affiliation info available
Frenich AG
No affiliation info available

MeSH

Anti-Bacterial AgentsChromatography, High Pressure LiquidDrug ResiduesHoneyTandem Mass Spectrometry

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19195999

Citation

Vidal, Jose Luis Martínez, et al. "Multiclass Analysis of Antibiotic Residues in Honey By Ultraperformance Liquid Chromatography-tandem Mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 57, no. 5, 2009, pp. 1760-7.
Vidal JL, Aguilera-Luiz Mdel M, Romero-González R, et al. Multiclass analysis of antibiotic residues in honey by ultraperformance liquid chromatography-tandem mass spectrometry. J Agric Food Chem. 2009;57(5):1760-7.
Vidal, J. L., Aguilera-Luiz, M. d. e. l. . M., Romero-González, R., & Frenich, A. G. (2009). Multiclass analysis of antibiotic residues in honey by ultraperformance liquid chromatography-tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 57(5), 1760-7. https://doi.org/10.1021/jf8034572
Vidal JL, et al. Multiclass Analysis of Antibiotic Residues in Honey By Ultraperformance Liquid Chromatography-tandem Mass Spectrometry. J Agric Food Chem. 2009 Mar 11;57(5):1760-7. PubMed PMID: 19195999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiclass analysis of antibiotic residues in honey by ultraperformance liquid chromatography-tandem mass spectrometry. AU - Vidal,Jose Luis Martínez, AU - Aguilera-Luiz,María Del Mar, AU - Romero-González,Roberto, AU - Frenich,Antonia Garrido, PY - 2009/2/7/entrez PY - 2009/2/7/pubmed PY - 2010/8/18/medline SP - 1760 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 5 N2 - A method has been developed and validated for the simultaneous analysis of different veterinary drug residues (macrolides, tetracyclines, quinolones, and sulfonamides) in honey. Honey samples were dissolved with Na(2)EDTA, and veterinary residues were extracted from the supernatant by solid-phase extraction (SPE), using OASIS HLB cartridges. The separation and determination was carried out by ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS), using an electrospay ionization source (ESI) in positive mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of sensitivity and specificity. The method was validated, and mean recoveries were evaluated at three concentration levels (10, 50, and 100 microg/kg), ranging from 70 to 120% except for doxycycline, erythromycin, and tylmicosin with recovery higher than 50% at the three levels assayed. Relative standard deviations (RSDs) of the recoveries were less than 20% within the intraday precision and less than 25% within the interday precision. The limits of quantification (LOQs) were always lower than 4 microg/kg. The developed procedure was applied to 16 honey samples, and erythromycin, sarafloxacin, and tylosin were found in a few samples. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19195999/Multiclass_analysis_of_antibiotic_residues_in_honey_by_ultraperformance_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://doi.org/10.1021/jf8034572 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓5]

Related Citations

More