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Exogenous IL-12 and its effect on TH1/TH2 cell activity after cardiac surgery.
Shock. 2009 Oct; 32(4):366-73.S

Abstract

There is controversy about the origin of the alterations in T helper 1 (TH1)/TH2 cell activity after major surgical procedures such as on-pump cardiac surgery. We hypothesized that a postoperative decrease in interferon (IFN) gamma-producing TH1 lymphocyte activity may be the sole cause of this TH1/TH2 shift and that the addition of recombinant IL-12 can reverse TH1 suppression. Peripheral blood mononuclear cell fractions from 20 low-risk elective cardiac surgery patients were analyzed preoperatively (d0) and on the first (d1), third (d3), and sixth (d6) postoperative days. We determined the absolute numbers of T helper lymphocytes, IFN-gamma-producing TH1 cells, and IL-4-producing TH2 cells after stimulation and measured IFN-gamma and IL-4 levels in the supernatants of stimulated peripheral blood mononuclear cell cultures, absolute monocyte counts, human leukocyte antigen-DR expression, and intracellular IL-12 synthesis under comparable conditions. Recombinant IL-12 alone or in combination with a neutralizing antibody was added. T helper lymphocyte counts were reduced postoperatively from d1 to d6 (P < 0.05). Absolute IFN-gamma- and IL-4-positive T helper lymphocyte counts were reduced on d1 (P < 0.05). Intracellular IL-4 production in T helper lymphocytes remained postoperatively unchanged. Interferon gamma synthesis was significantly reduced until d3 (P = 0.001) and significantly increased after IL-12 addition (P < 0.05). This effect was reversed by the addition of a neutralizing anti-IL-12 antibody. The TH1/TH2 shift after cardiac surgery seems to be caused primarily by a decrease in cellular IFN-gamma synthesis in TH1 lymphocytes. Because TH1 suppression can be reversed by IL-12, it is more likely to be the result of altered stimulation pathways than cellular defects.

Authors+Show Affiliations

Department of Trauma and Reconstructive Surgery, German Armed Forces Central Hospital, Koblenz, Germany. dr.axel.franke@t-online.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19197230

Citation

Franke, Axel, et al. "Exogenous IL-12 and Its Effect On TH1/TH2 Cell Activity After Cardiac Surgery." Shock (Augusta, Ga.), vol. 32, no. 4, 2009, pp. 366-73.
Franke A, Lante W, Kollig E, et al. Exogenous IL-12 and its effect on TH1/TH2 cell activity after cardiac surgery. Shock. 2009;32(4):366-73.
Franke, A., Lante, W., Kollig, E., Koeller, M., Schinkel, C., & Markewitz, A. (2009). Exogenous IL-12 and its effect on TH1/TH2 cell activity after cardiac surgery. Shock (Augusta, Ga.), 32(4), 366-73. https://doi.org/10.1097/SHK.0b013e31819d82ad
Franke A, et al. Exogenous IL-12 and Its Effect On TH1/TH2 Cell Activity After Cardiac Surgery. Shock. 2009;32(4):366-73. PubMed PMID: 19197230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous IL-12 and its effect on TH1/TH2 cell activity after cardiac surgery. AU - Franke,Axel, AU - Lante,Wolfgang, AU - Kollig,Erwin, AU - Koeller,Manfred, AU - Schinkel,Christian, AU - Markewitz,Andreas, PY - 2009/2/7/entrez PY - 2009/2/7/pubmed PY - 2010/1/15/medline SP - 366 EP - 73 JF - Shock (Augusta, Ga.) JO - Shock VL - 32 IS - 4 N2 - There is controversy about the origin of the alterations in T helper 1 (TH1)/TH2 cell activity after major surgical procedures such as on-pump cardiac surgery. We hypothesized that a postoperative decrease in interferon (IFN) gamma-producing TH1 lymphocyte activity may be the sole cause of this TH1/TH2 shift and that the addition of recombinant IL-12 can reverse TH1 suppression. Peripheral blood mononuclear cell fractions from 20 low-risk elective cardiac surgery patients were analyzed preoperatively (d0) and on the first (d1), third (d3), and sixth (d6) postoperative days. We determined the absolute numbers of T helper lymphocytes, IFN-gamma-producing TH1 cells, and IL-4-producing TH2 cells after stimulation and measured IFN-gamma and IL-4 levels in the supernatants of stimulated peripheral blood mononuclear cell cultures, absolute monocyte counts, human leukocyte antigen-DR expression, and intracellular IL-12 synthesis under comparable conditions. Recombinant IL-12 alone or in combination with a neutralizing antibody was added. T helper lymphocyte counts were reduced postoperatively from d1 to d6 (P < 0.05). Absolute IFN-gamma- and IL-4-positive T helper lymphocyte counts were reduced on d1 (P < 0.05). Intracellular IL-4 production in T helper lymphocytes remained postoperatively unchanged. Interferon gamma synthesis was significantly reduced until d3 (P = 0.001) and significantly increased after IL-12 addition (P < 0.05). This effect was reversed by the addition of a neutralizing anti-IL-12 antibody. The TH1/TH2 shift after cardiac surgery seems to be caused primarily by a decrease in cellular IFN-gamma synthesis in TH1 lymphocytes. Because TH1 suppression can be reversed by IL-12, it is more likely to be the result of altered stimulation pathways than cellular defects. SN - 1540-0514 UR - https://www.unboundmedicine.com/medline/citation/19197230/Exogenous_IL_12_and_its_effect_on_TH1/TH2_cell_activity_after_cardiac_surgery_ L2 - https://doi.org/10.1097/SHK.0b013e31819d82ad DB - PRIME DP - Unbound Medicine ER -