Tags

Type your tag names separated by a space and hit enter

Experimental acute respiratory Burkholderia pseudomallei infection in BALB/c mice.
Int J Exp Pathol. 2009 Feb; 90(1):16-25.IJ

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis, which is considered a potential deliberate release agent. The objective of this study was to establish and characterise a relevant, acute respiratory Burkholderia pseudomallei infection in BALB/c mice. Mice were infected with 100 B. pseudomallei strain BRI bacteria by the aerosol route (approximately 20 median lethal doses). Bacterial counts within lung, liver, spleen, brain, kidney and blood over 5 days were determined and histopathological and immunocytochemical profiles were assessed. Bacterial numbers in the lungs reached approximately 10(8) cfu/ml at day 5 post-infection. Bacterial numbers in other tissues were lower, reaching between 10(3) and 10(5) cfu/ml at day 4. Blood counts remained relatively constant at approximately 1.0 x 10(2) cfu/ml. Foci of acute inflammation and necrosis were seen within lungs, liver and spleen. These results suggest that the BALB/c mouse is highly susceptible to B. pseudomallei by the aerosol route and represents a relevant model system of acute human melioidosis.

Authors+Show Affiliations

Biomedical Sciences, Defence Science and Technology Laboratory (Dstl), Porton Down, Salisbury, UK. mslever@dstl.gov.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19200247

Citation

Lever, Mark S., et al. "Experimental Acute Respiratory Burkholderia Pseudomallei Infection in BALB/c Mice." International Journal of Experimental Pathology, vol. 90, no. 1, 2009, pp. 16-25.
Lever MS, Nelson M, Stagg AJ, et al. Experimental acute respiratory Burkholderia pseudomallei infection in BALB/c mice. Int J Exp Pathol. 2009;90(1):16-25.
Lever, M. S., Nelson, M., Stagg, A. J., Beedham, R. J., & Simpson, A. J. (2009). Experimental acute respiratory Burkholderia pseudomallei infection in BALB/c mice. International Journal of Experimental Pathology, 90(1), 16-25. https://doi.org/10.1111/j.1365-2613.2008.00619.x
Lever MS, et al. Experimental Acute Respiratory Burkholderia Pseudomallei Infection in BALB/c Mice. Int J Exp Pathol. 2009;90(1):16-25. PubMed PMID: 19200247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental acute respiratory Burkholderia pseudomallei infection in BALB/c mice. AU - Lever,Mark S, AU - Nelson,Michelle, AU - Stagg,Anthony J, AU - Beedham,Richard J, AU - Simpson,Andrew J H, PY - 2009/2/10/entrez PY - 2009/2/10/pubmed PY - 2009/5/15/medline SP - 16 EP - 25 JF - International journal of experimental pathology JO - Int J Exp Pathol VL - 90 IS - 1 N2 - Burkholderia pseudomallei is the causative agent of melioidosis, which is considered a potential deliberate release agent. The objective of this study was to establish and characterise a relevant, acute respiratory Burkholderia pseudomallei infection in BALB/c mice. Mice were infected with 100 B. pseudomallei strain BRI bacteria by the aerosol route (approximately 20 median lethal doses). Bacterial counts within lung, liver, spleen, brain, kidney and blood over 5 days were determined and histopathological and immunocytochemical profiles were assessed. Bacterial numbers in the lungs reached approximately 10(8) cfu/ml at day 5 post-infection. Bacterial numbers in other tissues were lower, reaching between 10(3) and 10(5) cfu/ml at day 4. Blood counts remained relatively constant at approximately 1.0 x 10(2) cfu/ml. Foci of acute inflammation and necrosis were seen within lungs, liver and spleen. These results suggest that the BALB/c mouse is highly susceptible to B. pseudomallei by the aerosol route and represents a relevant model system of acute human melioidosis. SN - 1365-2613 UR - https://www.unboundmedicine.com/medline/citation/19200247/Experimental_acute_respiratory_Burkholderia_pseudomallei_infection_in_BALB/c_mice_ L2 - https://doi.org/10.1111/j.1365-2613.2008.00619.x DB - PRIME DP - Unbound Medicine ER -