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On-line molecularly imprinted solid-phase extraction for the selective spectrophotometric determination of nicotine in the urine of smokers.
Anal Chim Acta. 2009 Mar 02; 635(1):102-7.AC

Abstract

This work describes an on-line molecularly imprinted solid-phase extraction (MISPE) method for spectrophotometric determination of nicotine in urine samples of smokers. This method is based on manganese (VII) to manganese (VI) reduction in an alkaline medium, promoted by nicotine. Two wash solutions (1:4 (v/v) acetonitrile:sodium hydroxide--pH 11.4, and nitric acid--pH 2.5) were employed to circumvent interferences. Aqueous solutions containing nicotine plus different possible concomitants (cotinine, anabasine, norcotinine and caffeine) were tested individually. The analytical calibration curve was prepared in urine samples collected from non-smokers and spiked with nicotine standard from 1.1 to 60 micromol L(-1) (r(2)>0.998). The limit of quantification and the analytical frequency were 1.1 micromol L(-1) and 11 h(-1), respectively. The precision, evaluated using 3, 10 and 30 micromol L(-1) nicotine in urine, was 10, 10 and 4% (intra-day precision) and 12, 13 and 5% (inter-day precision), respectively. Accuracy was checked through high performance liquid chromatography and the results did not present significant differences at the 95% confidence level according to the Student's t-test.

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Authors+Show Affiliations

Figueiredo EC
Group of Spectrometry, Sample Preparation and Mechanization-GEPAM, Institute of Chemistry, P.O. Box 6154, University of Campinas, 13083-970 Campinas, SP, Brazil.
de Oliveira DM
No affiliation info available
de Siqueira ME
No affiliation info available
Arruda MA
No affiliation info available

MeSH

BuffersCalibrationHumansHydrogen-Ion ConcentrationNicotineReproducibility of ResultsSensitivity and SpecificitySmokingSpectrophotometryTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19200485

Citation

Figueiredo, Eduardo Costa, et al. "On-line Molecularly Imprinted Solid-phase Extraction for the Selective Spectrophotometric Determination of Nicotine in the Urine of Smokers." Analytica Chimica Acta, vol. 635, no. 1, 2009, pp. 102-7.
Figueiredo EC, de Oliveira DM, de Siqueira ME, et al. On-line molecularly imprinted solid-phase extraction for the selective spectrophotometric determination of nicotine in the urine of smokers. Anal Chim Acta. 2009;635(1):102-7.
Figueiredo, E. C., de Oliveira, D. M., de Siqueira, M. E., & Arruda, M. A. (2009). On-line molecularly imprinted solid-phase extraction for the selective spectrophotometric determination of nicotine in the urine of smokers. Analytica Chimica Acta, 635(1), 102-7. https://doi.org/10.1016/j.aca.2008.12.045
Figueiredo EC, et al. On-line Molecularly Imprinted Solid-phase Extraction for the Selective Spectrophotometric Determination of Nicotine in the Urine of Smokers. Anal Chim Acta. 2009 Mar 2;635(1):102-7. PubMed PMID: 19200485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line molecularly imprinted solid-phase extraction for the selective spectrophotometric determination of nicotine in the urine of smokers. AU - Figueiredo,Eduardo Costa, AU - de Oliveira,Daniela Mayra, AU - de Siqueira,Maria Elisa Pereira Bastos, AU - Arruda,Marco Aurélio Zezzi, Y1 - 2009/01/08/ PY - 2008/07/16/received PY - 2008/12/22/revised PY - 2008/12/26/accepted PY - 2009/2/10/entrez PY - 2009/2/10/pubmed PY - 2009/3/11/medline SP - 102 EP - 7 JF - Analytica chimica acta JO - Anal Chim Acta VL - 635 IS - 1 N2 - This work describes an on-line molecularly imprinted solid-phase extraction (MISPE) method for spectrophotometric determination of nicotine in urine samples of smokers. This method is based on manganese (VII) to manganese (VI) reduction in an alkaline medium, promoted by nicotine. Two wash solutions (1:4 (v/v) acetonitrile:sodium hydroxide--pH 11.4, and nitric acid--pH 2.5) were employed to circumvent interferences. Aqueous solutions containing nicotine plus different possible concomitants (cotinine, anabasine, norcotinine and caffeine) were tested individually. The analytical calibration curve was prepared in urine samples collected from non-smokers and spiked with nicotine standard from 1.1 to 60 micromol L(-1) (r(2)>0.998). The limit of quantification and the analytical frequency were 1.1 micromol L(-1) and 11 h(-1), respectively. The precision, evaluated using 3, 10 and 30 micromol L(-1) nicotine in urine, was 10, 10 and 4% (intra-day precision) and 12, 13 and 5% (inter-day precision), respectively. Accuracy was checked through high performance liquid chromatography and the results did not present significant differences at the 95% confidence level according to the Student's t-test. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/19200485/On_line_molecularly_imprinted_solid_phase_extraction_for_the_selective_spectrophotometric_determination_of_nicotine_in_the_urine_of_smokers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(09)00002-6 DB - PRIME DP - Unbound Medicine ER -