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Expression of porcine Toll-like receptor 2, 4 and 9 gene transcripts in the presence of lipopolysaccharide and Salmonella enterica serovars Typhimurium and Choleraesuis.
Vet Immunol Immunopathol. 2009 Jul 15; 130(1-2):96-101.VI

Abstract

Salmonella enterica serovar Typhimurium (ST) and Choleraesuis (SC) are among the most frequently isolated salmonellae serovars causing enteric disease in swine. Enteric disease in young pigs is of major concern in modern production systems due to the negative implications on animal health, food safety and economic return. Epithelial cells express Toll-like receptors (TLR) that recognize conserved microbial structures and act as mediators of innate and adaptive immune responses. However, little is known about the expression of TLR gene transcripts in swine. The objective of the current study was to characterize the relative abundance of porcine TLR2, 4 and 9 gene transcripts in vitro in a porcine jejunal epithelial cell line (IPEC-J2) and in porcine mononuclear phagocytes (pMP) in the presence of ST or SC, as well as in vivo in the distal ileum of pigs orally challenged with ST. Our results indicate that TLR2, 4 and 9 are constitutively expressed in vitro in IPEC-J2 cells and pMP and in vivo in the distal ileum. Additionally, transient modulation of porcine TLR was observed in vitro and in vivo in the presence of ST and SC. Further investigation is warranted to determine the effects of ST and SC on functional TLR.

Authors+Show Affiliations

Animal Science Department, University of Nebraska, C206F Animal Science, Lincoln, NE 68583, USA. tburkey2@unl.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19200608

Citation

Burkey, T E., et al. "Expression of Porcine Toll-like Receptor 2, 4 and 9 Gene Transcripts in the Presence of Lipopolysaccharide and Salmonella Enterica Serovars Typhimurium and Choleraesuis." Veterinary Immunology and Immunopathology, vol. 130, no. 1-2, 2009, pp. 96-101.
Burkey TE, Skjolaas KA, Dritz SS, et al. Expression of porcine Toll-like receptor 2, 4 and 9 gene transcripts in the presence of lipopolysaccharide and Salmonella enterica serovars Typhimurium and Choleraesuis. Vet Immunol Immunopathol. 2009;130(1-2):96-101.
Burkey, T. E., Skjolaas, K. A., Dritz, S. S., & Minton, J. E. (2009). Expression of porcine Toll-like receptor 2, 4 and 9 gene transcripts in the presence of lipopolysaccharide and Salmonella enterica serovars Typhimurium and Choleraesuis. Veterinary Immunology and Immunopathology, 130(1-2), 96-101. https://doi.org/10.1016/j.vetimm.2008.12.027
Burkey TE, et al. Expression of Porcine Toll-like Receptor 2, 4 and 9 Gene Transcripts in the Presence of Lipopolysaccharide and Salmonella Enterica Serovars Typhimurium and Choleraesuis. Vet Immunol Immunopathol. 2009 Jul 15;130(1-2):96-101. PubMed PMID: 19200608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of porcine Toll-like receptor 2, 4 and 9 gene transcripts in the presence of lipopolysaccharide and Salmonella enterica serovars Typhimurium and Choleraesuis. AU - Burkey,T E, AU - Skjolaas,K A, AU - Dritz,S S, AU - Minton,J E, Y1 - 2009/01/03/ PY - 2006/04/13/received PY - 2008/12/23/revised PY - 2008/12/24/accepted PY - 2009/2/10/entrez PY - 2009/2/10/pubmed PY - 2009/11/3/medline SP - 96 EP - 101 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 130 IS - 1-2 N2 - Salmonella enterica serovar Typhimurium (ST) and Choleraesuis (SC) are among the most frequently isolated salmonellae serovars causing enteric disease in swine. Enteric disease in young pigs is of major concern in modern production systems due to the negative implications on animal health, food safety and economic return. Epithelial cells express Toll-like receptors (TLR) that recognize conserved microbial structures and act as mediators of innate and adaptive immune responses. However, little is known about the expression of TLR gene transcripts in swine. The objective of the current study was to characterize the relative abundance of porcine TLR2, 4 and 9 gene transcripts in vitro in a porcine jejunal epithelial cell line (IPEC-J2) and in porcine mononuclear phagocytes (pMP) in the presence of ST or SC, as well as in vivo in the distal ileum of pigs orally challenged with ST. Our results indicate that TLR2, 4 and 9 are constitutively expressed in vitro in IPEC-J2 cells and pMP and in vivo in the distal ileum. Additionally, transient modulation of porcine TLR was observed in vitro and in vivo in the presence of ST and SC. Further investigation is warranted to determine the effects of ST and SC on functional TLR. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/19200608/Expression_of_porcine_Toll_like_receptor_2_4_and_9_gene_transcripts_in_the_presence_of_lipopolysaccharide_and_Salmonella_enterica_serovars_Typhimurium_and_Choleraesuis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(08)00814-3 DB - PRIME DP - Unbound Medicine ER -