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Recombinant human erythropoietin attenuates spinal neuroimmune activation of neuropathic pain in rats.
Ann Clin Lab Sci. 2009 Winter; 39(1):84-91.AC

Abstract

Neuropathic pain is a complex syndrome resulting from damage to the peripheral nervous system. Central neuroimmune activation contributes to the generation and maintenance of chronic pain after nerve injury. The current study determined the effects of recombinant human erythropoietin (rhEPO) on behavioral hyperalgesia and neuroimmune activation in a rat model of neuropathic pain induced by L5 spinal nerve transection. Animals were randomly assigned into 3 groups: sham-operation with saline; L5 spinal nerve transection with rhEPO (5000 units/kg); or L5 transection with saline. The rhEPO or saline was given ip on the day before surgery and continued daily to day 7 post-transection. The paw pressure threshold and paw withdrawal latencies were measured before surgery and on days 1, 3, and 7 post-operation. Glial activation markers such as macrophage antigen complex-1 (Mac-1, OX-42) and glial fibrillary acidic protein (GFAP), production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-10, as well as nuclear factor-kappa B (NF-kappaB) activation were determined in the lumbar spinal cord. Administration of rhEPO resulted in attenuation of mechanical and thermal hyperalgesia. Furthermore, rhEPO markedly inhibited neuroimmune activation characterized by glial activation, production of proinflammatory cytokines like TNF-alpha, IL-1beta, and NF-kappaB activation, but rhEPO enhanced the level of IL-10. These results support the significance of neuroinflammation and neuroimmune activation in the initiation and persistence of behavioral pain responses. The data indicate that rhEPO attenuates behavioral hyperalgesia and neuroimmune activation in neuropathic pain induced by L5 nerve transection.

Authors+Show Affiliations

Department of Anesthesiology, Jinling Hospital, Nanjing Clinical Medical College of the Second Military Medical University, Nanjing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19201747

Citation

Jia, Hongbin, et al. "Recombinant Human Erythropoietin Attenuates Spinal Neuroimmune Activation of Neuropathic Pain in Rats." Annals of Clinical and Laboratory Science, vol. 39, no. 1, 2009, pp. 84-91.
Jia H, Feng X, Li W, et al. Recombinant human erythropoietin attenuates spinal neuroimmune activation of neuropathic pain in rats. Ann Clin Lab Sci. 2009;39(1):84-91.
Jia, H., Feng, X., Li, W., Hu, Y., Zeng, Q., Liu, J., & Xu, J. (2009). Recombinant human erythropoietin attenuates spinal neuroimmune activation of neuropathic pain in rats. Annals of Clinical and Laboratory Science, 39(1), 84-91.
Jia H, et al. Recombinant Human Erythropoietin Attenuates Spinal Neuroimmune Activation of Neuropathic Pain in Rats. Ann Clin Lab Sci. 2009;39(1):84-91. PubMed PMID: 19201747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant human erythropoietin attenuates spinal neuroimmune activation of neuropathic pain in rats. AU - Jia,Hongbin, AU - Feng,Xiaomei, AU - Li,Weiyan, AU - Hu,Yufeng, AU - Zeng,Qiong, AU - Liu,Jian, AU - Xu,Jianguo, PY - 2009/2/10/entrez PY - 2009/2/10/pubmed PY - 2009/3/26/medline SP - 84 EP - 91 JF - Annals of clinical and laboratory science JO - Ann Clin Lab Sci VL - 39 IS - 1 N2 - Neuropathic pain is a complex syndrome resulting from damage to the peripheral nervous system. Central neuroimmune activation contributes to the generation and maintenance of chronic pain after nerve injury. The current study determined the effects of recombinant human erythropoietin (rhEPO) on behavioral hyperalgesia and neuroimmune activation in a rat model of neuropathic pain induced by L5 spinal nerve transection. Animals were randomly assigned into 3 groups: sham-operation with saline; L5 spinal nerve transection with rhEPO (5000 units/kg); or L5 transection with saline. The rhEPO or saline was given ip on the day before surgery and continued daily to day 7 post-transection. The paw pressure threshold and paw withdrawal latencies were measured before surgery and on days 1, 3, and 7 post-operation. Glial activation markers such as macrophage antigen complex-1 (Mac-1, OX-42) and glial fibrillary acidic protein (GFAP), production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-10, as well as nuclear factor-kappa B (NF-kappaB) activation were determined in the lumbar spinal cord. Administration of rhEPO resulted in attenuation of mechanical and thermal hyperalgesia. Furthermore, rhEPO markedly inhibited neuroimmune activation characterized by glial activation, production of proinflammatory cytokines like TNF-alpha, IL-1beta, and NF-kappaB activation, but rhEPO enhanced the level of IL-10. These results support the significance of neuroinflammation and neuroimmune activation in the initiation and persistence of behavioral pain responses. The data indicate that rhEPO attenuates behavioral hyperalgesia and neuroimmune activation in neuropathic pain induced by L5 nerve transection. SN - 1550-8080 UR - https://www.unboundmedicine.com/medline/citation/19201747/Recombinant_human_erythropoietin_attenuates_spinal_neuroimmune_activation_of_neuropathic_pain_in_rats_ L2 - http://www.annclinlabsci.org/cgi/pmidlookup?view=long&pmid=19201747 DB - PRIME DP - Unbound Medicine ER -